Chiral carbon quantum dots for optical power limiting application.

In this research, the introduction of chirality into carbon quantum dots (CQDs) successfully facilitated the formation of chiral carbon quantum dots (CCQDs). These CCQDs manifested excellent photophysical properties. Moreover, chirality was exploited as a key factor to modulate the optical power limiting (OPL) properties of the CQDs.

Advancements in glycan analysis: high performance anion exchange chromatography-pulsed amprometric detection coupled with mass spectrometry for structural elucidation.

Carbohydrates are essential biomolecules that play a vital role in various biological processes across humans, plants, and bacteria. Despite their ubiquity, the structural elucidation of carbohydrates, particularly oligo- and polysaccharides, remains a significant challenge due to their complex and heterogeneous nature. The high-performance anion exchange chromatography (HPAEC) or called ion chromatography (IC) coupled with pulsed amperometric detection (PAD) has emerged as a powerful tool for highly effective separation and highly specific detection of glycans.

The incidence and risk factors of gastrointestinal dysfunction during enteral nutrition in mechanically ventilated critically ill patients.

Aim: To assess the occurrence and risk factors of gastrointestinal (GI) dysfunction during enteral nutrition (EN) in critically ill patients supported with mechanical ventilation.

Design: Prospective observational study.

Methods: Totally 252 patients admitted at a mixed medical-surgical ICU were enrolled.

Effect of Implementing an Informatization Case Management Model on the Management of Chronic Respiratory Diseases in a General Hospital: Retrospective Controlled Study.

Background: The use of chronic disease information systems in hospitals and communities plays a significant role in disease prevention, control, and monitoring. However, there are several limitations to these systems, including that the platforms are generally isolated, the patient health information and medical resources are not effectively integrated, and the "Internet Plus Healthcare" technology model is not implemented throughout the patient consultation process.

Objective: The aim of this study was to evaluate the efficiency of the application of a hospital case management information system in a general hospital in the context of chronic respiratory diseases as a model case.

Responsive Regulation of Energy Transfer in Lanthanide-Doped Nanomaterials Dispersed in Chiral Nematic Structure.

The responsive control of energy transfer (ET) plays a key role in the broad applications of lanthanide-doped nanomaterials. Photonic crystals (PCs) are excellent materials for ET regulation. Among the numerous materials that can be used to fabricate PCs, chiral nematic liquid crystals are highly attractive due to their good photoelectric responsiveness and biocompatibility.

Peroxymonosulfate activation by CuFe-prussian blue analogues for the degradation of bisphenol S: Effect, mechanism, and pathway.

The fenton-like process based on peroxymonosulfate (PMS) activation is considered as a promising strategy for the removal of organic pollutants. However, the development of efficient photocatalysts for PMS activation remains challenging. Herein, copper-iron prussian blue analogue (CuFe-PBA, n = 1, 2, 3, 4) nanomaterials were first fabricated through a simple combination of co-precipitation and calcination processes.

Luminescence regulation of lanthanide-doped nanorods in chiral photonic cellulose nanocrystal films.

A new design for chiral photonic cellulose nanocrystal films was developed by co-assembling lanthanide-doped nanorods (NRs) into chiral cellulose nanocrystals, in which the photonic band gap (PBG) could be tuned in the visible range by changing the mass fraction of flexible agents, such as polyvinyl alcohol (PVA) and ethylene glycol (EG). Due to the PBG effect, the luminescence modulation in such nanocrystal films had been realized. The down-conversion luminescence from NaGdYF:5%Tb, 5%Eu NRs and up-conversion luminescence from NaGdYF:18%Yb, 2%Er NRs could be enhanced by 28 % and 18 % respectively, on account of the band edge effect.

Degradation mechanism of ammonia nitrogen synergistic with bromate under UV or UV/TiO.

Bromate (BrO) and ammonia nitrogen (NH) are both typical environmental pollutants: BrO has been categorized as one of the Group 2B carcinogen by IARC; an excess of NH might result in the eutrophication of water. The existence of NH could inhibit the transformation of bromide (Br) to bromate (BrO). However, the interaction of NH and BrO during the removal process is not clear.

Genome-Wide Study of Gene Family in Cabbage ( var. L.) and Their Imperative Roles in Response to Cold Stress.

Heat shock protein 90 (Hsp90) plays an important role in plant developmental regulation and defensive reactions. Several plant species have been examined for the family gene. However, the gene family in cabbage has not been well investigated to date.

CREB1 contributes colorectal cancer cell plasticity by regulating lncRNA CCAT1 and NF-κB pathways.

The CREB1 gene encodes an exceptionally pleiotropic transcription factor that frequently dysregulated in human cancers. CREB1 can regulate tumor cell status of proliferation and/or migration; however, the molecular basis for this switch involvement in cell plasticity has not fully been understood yet. Here, we first show that knocking out CREB1 triggers a remarkable effect of epithelial-mesenchymal transition (EMT) and leads to the occurrence of inhibited proliferation and enhanced motility in HCT116 colorectal cancer cells.

Importance of Volume Ratio in Photonic Effects of Lanthanide-Doped LaPO Nanocrystals.

Experimental variation of the volume ratio (filling factor: i.e., volume of nanoparticles (NPs) compared with that of medium) of nanocomposite materials with doped lanthanide ions demonstrates that it has a significant affect upon local field effects.

Structure and DNA damage-dependent derepression mechanism for the XRE family member DG-DdrO.

DdrO is an XRE family transcription repressor that, in coordination with the metalloprotease PprI, is critical in the DNA damage response of Deinococcus species. Here, we report the crystal structure of Deinococcus geothermalis DdrO. Biochemical and structural studies revealed the conserved recognizing α-helix and extended dimeric interaction of the DdrO protein, which are essential for promoter DNA binding.

Energy Transfer between Tb and Eu in LaPO: Pulsed versus Switched-off Continuous Wave Excitation.

The energy transfer (ET) between Tb and Eu is investigated experimentally and with available theoretical models in the regime of high Tb concentrations in ≈30 nm LaPO nanoparticles at room temperature. The ET efficiency approaches 100% even for lightly Eu-doped materials. The major conclusion from the use of pulsed laser excitation and switched-off continuous wave laser diode excitation is that the energy migration between Tb ions, situated on La sites with ≈4 Å separation, is not fast.

Effects of europium spectral probe interchange in Ln-dyads with cyclen and phen moieties.

Herein, we have investigated spectral structure and intensity changes in a bimetallic lanthanide complex comprising La3+ and Eu3+, with the ions coordinated to silent and antenna ligands, when their positions are interchanged. Comparison of the fluorescence decay of a ligand in the presence and absence of La3+ has enabled internal nonradiative decay rates to be determined. The effects upon Eu3+ emission spectra resulting from changes in its environment at a distance of ∼10 Å, and upon changing from the solid state to solution, were also investigated.

Massive Stokes shift in 12-coordinate Ce(NO2): crystal structure, vibrational and electronic spectra.

The Ce ion in CsNaCe(NO) (I), which comprises the unusual T site symmetry of the Ce(NO) ion, demonstrates the largest Ce-O Stokes shift of 8715 cm and the low emission quenching temperature of 53 K. The activation energy for quenching changes with temperature, attributed to relative shifts of the two potential energy curves involved. The splitting of the Ce 5d state into two levels separated by 4925 cm is accounted for by a first principles calculation using the crystal structure data of I.

Electronic Spectra of CsNaYb(NO): Is There Quantum Cutting?

The crystal structure and electronic spectra of the T symmetry hexanitritoytterbate(III) anion have been studied in CsNaYYb(NO), which crystallizes in the cubic space group Fm3̅. The emission from Yb can be excited via the NO antenna. The latter electronic transition is situated at more than twice the energy of the former, but at room temperature, one photon absorbed at 470 nm in the triplet state produces no more than one photon emitted.

Comprehensive Genomic Characterization of RNA-Binding Proteins across Human Cancers.

RNA-binding proteins (RBPs) regulate the expression of thousands of transcripts, and some are reported to be involved in human tumorigenesis. However, little is known about the dysregulation of RBPs at the genomic level in human cancers. Here, we conducted comprehensive analyses for expression, somatic copy number alteration (SCNA), and mutation profiles of 1,542 RBPs in ∼7,000 clinical specimens across 15 cancer types.

A Smart Europium-Ruthenium Complex as Anticancer Prodrug: Controllable Drug Release and Real-Time Monitoring under Different Light Excitations.

A unique, dual-function, photoactivatable anticancer prodrug, RuEuL, has been tailored that features a ruthenium(II) complex linked to a cyclen-europium chelate via a π-conjugated bridge. Under irradiation at 488 nm, the dark-inactive prodrug undergoes photodissociation, releasing the DNA-damaging ruthenium species. Under evaluation-window irradiation (λ = one-photon 350 nm or two-photon 700 nm), the drug delivery process can be quantitatively monitored in real-time because of the long-lived red europium emission.

AAVS1-Targeted Plasmid Integration in AAV Producer Cell Lines.

Adeno-associated virus (AAV) producer cell lines are created via transfection of HeLaS3 cells with a single plasmid containing three components (the vector sequence, the AAV rep and cap genes, and a selectable marker gene). As this plasmid contains both the cis (Rep binding sites) and trans (Rep protein encoded by the rep gene) elements required for site-specific integration, it was predicted that plasmid integration might occur within the AAVS1 locus on human chromosome 19 (chr19). The objective of this study was to investigate whether integration in AAVS1 might be correlated with vector yield.

Generation and characterization of adeno-associated virus producer cell lines for research and preclinical vector production.

Adeno-associated virus (AAV) producer cell lines represent an effective method for large-scale production of AAV vectors. We set out to evaluate and characterize the use of an abbreviated protocol to generate "masterwells" (MWs; a nonclonal cell population) as a platform for research and preclinical vector production. In this system, a single plasmid containing three components, the vector sequence, the AAV rep, and cap genes, and a selectable marker gene is stably transfected into HeLaS3 cells.

A constitutively active hypoxia-inducible factor-1alpha/VP16 hybrid factor activates expression of the human B-type natriuretic peptide gene.

Hypoxia-inducible factor-1 (HIF-1) is a primary regulator of the physiological response to hypoxia. A recombinant adenovirus expressing a constitutively active hybrid form of the HIF-1alpha subunit (Ad2/HIF-1alpha/VP16) is being evaluated as a gene therapy for the treatment of peripheral vascular disease. Ad2/HIF-1alpha/VP16 up-regulates known HIF-1-responsive genes, including those involved in angiogenesis.

Stabilization of vascular endothelial growth factor mRNA by hypoxia-inducible factor 1.

Hypoxia regulates expression of vascular endothelial growth factor (VEGF) by increasing its transcription and by stabilizing its mRNA. Despite the pivotal role of hypoxia-inducible factor 1 (HIF-1) in transcriptional activation of hypoxia-responsive genes, it is not known whether HIF-1 mediates hypoxia-induced stabilization of VEGF mRNA. We constructed adenoviral vectors expressing either the wild-type HIF-1 alpha (Ad2/HIF-1 alpha/FL), a constitutively stable hybrid form of HIF-1 alpha (Ad2/HIF-1 alpha/VP16), or no transgene (Ad2/CMVEV).