Evaluation of an identification method for the SARS-CoV-2 Delta variant based on the amplification-refractory mutation system.

The Delta variant of SARS-CoV-2 dominated the COVID-19 pandemic due to its high viral replication capacity and immune evasion, causing massive outbreaks of cases, hospitalizations, and deaths. Currently, variant identification is performed mainly by sequencing. However, the high requirements for equipment and operators as well as its high cost have limited its application in underdeveloped regions.

High-throughput and high-dimensional single-cell analysis of antigen-specific CD8 T cells.

Although critical to T cell function, antigen specificity is often omitted in high-throughput multiomics-based T cell profiling due to technical challenges. We describe a high-dimensional, tetramer-associated T cell antigen receptor (TCR) sequencing (TetTCR-SeqHD) method to simultaneously profile cognate antigen specificities, TCR sequences, targeted gene expression and surface-protein expression from tens of thousands of single cells. Using human polyclonal CD8 T cells with known antigen specificity and TCR sequences, we demonstrate over 98% precision for detecting the correct antigen specificity.

Electroenzymatic choline sensing at near the theoretical performance limit.

A high performance, electroenzymatic microsensor for choline based on choline oxidase (ChOx) immobilized on Pt coated with permselective polymer layers has been created that exhibits sensitivity approaching the theoretical performance limit. Sensor construction was guided by simulations performed with a detailed mathematical model. Implantable microsensors with an array of electroenzymatic sensing sites provide a means to record concentration changes of choline, an effective surrogate for acetylcholine due to its very rapid turnover in the brain, and other neurochemicals in vivo.

Electroenzymatic glutamate sensing at near the theoretical performance limit.

The sensitivity and response time of glutamate sensors based on glutamate oxidase immobilized on planar platinum microelectrodes have been improved to near the theoretical performance limits predicted by a detailed mathematical model. Microprobes with an array of electroenzymatic sensing sites have emerged as useful tools for the monitoring of glutamate and other neurotransmitters in vivo; and implemented as such, they can be used to study many complex neurological diseases and disorders including Parkinson's disease and drug addiction. However, less than optimal sensitivity and response time has limited the spatiotemporal resolution of these promising research tools.

Corrosion inhibition of X65 steel in sulfuric acid by two food flavorants 2-isobutylthiazole and 1-(1,3-Thiazol-2-yl) ethanone as the green environmental corrosion inhibitors: Combination of experimental and theoretical researches.

Food flavors of 2-isobutylthiazole (ITT) and 1-(1,3-Thiazol-2-yl)ethanone (TEO) for the corrosion inhibition of X65 steel in HSO were studied by electrochemical methods, atomic force microscopy (AFM), scanning electron microscopy (SEM) and theoretical calculations. Electrochemical experiments show that ITT and TEO can effectively inhibit the corrosion of cathode and anode of X65 steel, and they are mixed-type corrosion inhibitors. Surface topography analysis (SEM and AFM) also visually demonstrate that ITT and TEO form an effective barrier film on the X65 steel surface to isolate the corrosive medium.