Glucose phosphorylated on carbon 6 suppresses lipopolysaccharide binding to lipopolysaccharide-binding protein and inhibits its bioactivities.

Lipid A comprises the active region of lipopolysaccharide (LPS), and its phosphate group is required for LPS activities. Additionally, it is essential for effects of inhibitors of LPS-induced coagulation activity in limulus amebocyte lysate (LAL) tests. Lipid A has phosphorylated glucosamine residues, which are structurally similar to glucose 1-phosphate (G1P) and glucose 6-phosphate (G6P).

The capture, hold and forward release of an optical pulse from a dynamic photonic crystal nanocavity.

We develop a silicon photonic crystal nanocavity device capable of performing targeted optical pulse capture and release via distinct ports on demand, based on dynamic Q factor control. The capture of 4 ps pulses and their release up to 332 ps later is directly observed by time-resolved measurements of the energy behaviour in both the nanocavity and emitted from the release port. We also discuss how the behaviour of excited free carriers dictates the performance of such dynamic devices.

Saline and buffers minimize the action of interfering factors in the bacterial endotoxins test.

The bacterial endotoxins test (BET) is the most sensitive assay for measuring endotoxin levels in solution. However, it is difficult to quantify endotoxin levels in some solutions because unknown interfering factors may inhibit or enhance the Limulus amebocyte lysate (LAL) coagulation reaction. We investigated the mechanisms of this interference and found that interference can be reduced or totally suppressed by preparing sample solutions in saline, Dulbecco's phosphate-buffered saline (D-PBS), N,N-bis-(2-hydroxyethyl)-2-aminoethanesulfonic acid (BES), or 2-amino-2-hydroxymethyl-1,3-propanediol (Tris) buffers.

Regulation of mitochondrial morphology through proteolytic cleavage of OPA1.

The dynamin-like GTPase OPA1, a causal gene product of human dominant optic atrophy, functions in mitochondrial fusion and inner membrane remodeling. It has several splice variants and even a single variant is found as several processed forms, although their functional significance is unknown. In yeast, mitochondrial rhomboid protease regulates mitochondrial function and morphology through proteolytic cleavage of Mgm1, the yeast homolog of OPA1.