Multi-pathway targeted therapy of MASH-HCC using miR-22.

Background: The treatment options for hepatocellular carcinoma (HCC) are limited, and there is no effective drug that can improve long-term survival rates. Complicated cocktails consisting of multiple medications with toxicities are frequently used to treat cancer. The current study addresses these challenges.

Patterns and variations of copy number alterations in acute myeloid leukemia: insights from the LeukAtlas database.

Recent pan-cancer analysis revealed the global pattern and potential aetiologies of copy number variation signatures in human cancers, particularly those derived from non-hematopoietic tissues. In sharp contrast, the generally low CNV burden in leukemia leaves the CNV landscape and variations largely unexplored, impeding understanding of CNV in leukemia development. Through a comprehensive compilation of public datasets, we constructed LeukAtlas ( https://ngdc.

Design, synthesis, and molecular docking of 4‑Thiol‑N‑acetylhexosamine uridine diphosphate donors for glycan synthesis.

Unnatural chemically modified nucleotide sugars UDP-4-SH-GalNAc was synthesized for the first time from a previously reported Benzyl 2-Amino-glucoypranoside derivative, with a total yield of 18.6%. UDP-4-SH-GalNAc was smaller in size than UDP-GalNAc calculated by Multiwfn, and its binding capability with glycosyltransferase was stronger than that of UDP-GalNAc and glycosyltransferase, which means the unnatural nucleotide sugars is equally well recognized by the enzyme and doped into the sugar chains.

A Medical Decision Support Platform for Identifying Thrombospondin 1 in Non-alcoholic Fatty Liver Disease via Immuno-Infiltration Analysis.

Non-alcoholic fatty liver disease (NAFLD) and myocardial infarction (MI) are two major health burdens with significant prevalence and mortality. This study aimed to explore the co-expressed genes to understand the relationship between NAFLD and MI and identify potential crucial biomarkers of NAFLD-related MI using bioinformatics and machine learning. Functional enrichment analysis was conducted, a co-protein-protein interaction (PPI) network diagram was constructed, and support vector machine-recursive feature elimination (SVM-RFE) and least absolute shrinkage and selection operator (LASSO) techniques were employed to identify one differentially expressed gene (DEG), Thrombospondin 1 (THBS1).

Lower risk of low bone mineral density in high vitamin E level in older people: A cross-sectional study.

Introduction: Osteoporosis and osteopenia, together known as low bone mineral density (LBMD), are common problems in the elderly. LBMD may cause fragility fractures in the elderly. The relationship between Vitamin E and LBMD in old Americans is still unclear.

Toward a Comprehensive Understanding of the Anomalously Small Contact Angle of Surface Nanobubbles.

Experimental studies have demonstrated that the gas phase contact angle (CA) of a surface nanobubble (SNB) is much smaller than that of a macroscopic gas bubble. This reduced CA plays a crucial role in prolonging the lifetime of SNBs by lowering the bubble pressure and preventing gas molecules from dissolving in the surrounding liquids. Despite extensive efforts to explain the anomalously small CA, a consensus about the underlying reasons is yet to be reached.

Extracellular vesicle-encapsulated microRNA-296-3p from cancer-associated fibroblasts promotes ovarian cancer development through regulation of the PTEN/AKT and SOCS6/STAT3 pathways.

Cancer-associated fibroblasts (CAFs), as important components of the tumor microenvironment, can regulate intercellular communication and tumor development by secreting extracellular vesicles (EVs). However, the role of CAF-derived EVs in ovarian cancer has not been fully elucidated. Here, using an EV-microRNA sequencing analysis, we reveal specific overexpression of microRNA (miR)-296-3p in activated CAF-derived EVs, which can be transferred to tumor cells to regulate the malignant phenotypes of ovarian cancer cells.

Adaptive evolution characteristics of mitochondrial genomes in genus Aparapotamon (Brachyura, Potamidae) of freshwater crabs.

Background: Aparapotamon, a freshwater crab genus endemic to China, includes 13 species. The distribution of Aparapotamon spans the first and second tiers of China's terrain ladder, showing great altitudinal differences. To study the molecular mechanisms of adaptive evolution in Aparapotamon, we performed evolutionary analyses, including morphological, geographical, and phylogenetic analyses and divergence time estimation.

Manipulating Trapped Nanobubbles Moving and Coalescing with Surface Nanobubbles.

Trapped nanobubbles are observed nucleating at nanopits on a pitted substrate, while surface nanobubbles are usually formed on the smooth solid surface in water. In this work, trapped nanobubbles and surface nanobubbles were captured by a tapping-mode atomic force microscope (AFM) on a nanopitted substrate based on the temperature difference method. A single trapped nanobubble was manipulated to change into a surface nanobubble, then to change into the trapped nanobubble again.

Algal/bacterial uptake kinetics of dissolved organic nitrogen in municipal wastewater treatment facilities effluents.

The simulation and analysis of the degradation process of organic nitrogen contaminants in wastewater treatment facility effluent is important to the estimation of its actual contribution to eutrophication, and it is crucial to the developing of watershed protection plan. In this study, algal and algal/bacterial based bioassay was conducted to study the bioavailability of dissolved organic nitrogen contaminants in wastewater treatment plants effluents, and 4 kinetic models were used to describe the mineralization process. The traditional 1-pool model that was commonly used in water quality models showed poor correlation (r = 0.

Relationship between water iodine and children's goiters.

Excessive iodine can lead to goiters. However, the relationship between the water iodine concentration (WIC) and goiter rate (GR) is unclear. This study aims to explore the factors that influence children's GR in areas with high WIC and analyse the threshold value of the GR increase associated with the WIC.

Characterization of the complete mitochondrial genome of (Hemiptera: Pentatomidae).

The is an important fruit pest in Chinese agricultural system. In current study, the complete mitochondrial genome of is determined. This mitogenome is 15,515 bp in size and comprises of 13 protein-coding genes, 22 transfer RNA genes, two ribosomal RNA genes, and a control region.

The mitochondrial genome of (Diptera: Dolichopodidae).

The long-legged fly belongs to the subfamily Xanthochlorinae of Dolichopodidae. The mitogenome of was sequenced, it is the first representative complete mitogenome from this subfamily. This mitogenome is 15,580 bp in size, includes 13 protein-coding genes, 22 transfer RNAs, and two ribosomal RNAs.

The complete mitochondrial genome of (Diptera: Dolichopodidae).

The long-legged fly belongs to the subfamily Neurigoninae of Dolichopodidae. The mitogenome (GenBank accession number: MT921956) of was sequenced, the first representative complete mitogenome from this subfamily. The complete mitogenome is 17,141 bp, includes 13 protein-coding genes, 2 rRNAs, and 22 transfer RNAs.

[TPM1 gene mutation is associated with dilated cardiomyopathy in Kazaks in Xinjiang].

Objective: Detect the relationship between TPM1 gene mutations and dilated cardiomyopathy (DCM) of Kazaks and Hans in Xinjiang.

Methods: TPM1 gene was screened from 31 family members in a Kazak family with familiar DCM (FDCM), 100 patients with idiopathic DCM (IDCM, 50 Kazaks and 50 Hans), and in 100 healthy controls (50 Kazaks and 50 Hans). All the samples were the inpatients or outpatients of First Affiliated Hospital of Xinjiang University from 2012 to 2014.

Altered expression of hyperpolarization-activated cyclic nucleotide-gated channels and microRNA-1 and -133 in patients with age-associated atrial fibrillation.

Hyperpolarization-activated cyclic nucleotide-gated (HCN) cation channels mediate pacemaker currents in the atrium. The microRNA (miR) families miR-1 and miR-133 regulate the expression of multiple genes involved in myocardial function, including HCN channels. It was hypothesized that age‑dependent changes in HCN2, HCN4, miR‑1 and miR‑133 expression may contribute to age‑associated atrial fibrillation, and therefore the correlation between expression levels, among adult (≤65 years) and aged patients (≥65 years), and sinus rhythm was determined.

Effects of ivabradine on cardiac electrophysiology in dogs with age-related atrial fibrillation.

Background: Ivabradine is an inhibitor of mixed Na+-K+ current that could combine with HCN channels to reduce the transmembrane velocity of funny current (If), heart rate, and cardiac efficiency, and thus be used for the treatment of cardiovascular diseases such as chronic heart failure. As an ion channel blocker, Ivabradine is also a potential antiarrhythmic agent.

Material/methods: Twelve aging dogs (8-10 years old) underwent rapid atrial pacing for 2 months to induce age-related AF in this study.

Association between reversal in the expression of hyperpolarization-activated cyclic nucleotide-gated (HCN) channel and age-related atrial fibrillation.

Background: We compared cardiac electrophysiological indicators and regional expression levels of cardiac hyperpolarization-activated cyclic nucleotide-gated (HCN) channels between adult and aged dogs to identify possible mechanisms of age-related atrial fibrillation.

Material/methods: Corrected sinus node recovery time (SNRTc) and effective refractory period (ERP) of the atrium and pulmonary veins were measured in 10 adult (3-6 years old) and 10 aged dogs (>9 years old). Expression levels of HCN2 and HCN4 channel mRNAs and proteins were measured in the sinoatrial node, atrium, and pulmonary veins by real-time PCR and Western blotting.