Antibacterial Activity, Probiotic Potential, and Biocontrol Efficacy of Two Lactic Acid Bacteria Against on Fresh Grapes.

Lactic acid bacteria are commonly present in various sources and possess significant probiotic properties. They can inhibit pathogenic bacteria and fungi simultaneously, making them promising candidates as bio-preservatives. This study investigated two potential probiotic strains: LR5-2 (isolated from fermented meat products) and SQ63 (isolated from infant feces).

Toward the Production of Super Graphene.

The quality requirements of graphene depend on the applications. Some have a high tolerance for graphene quality and even require some defects, while others require graphene as perfect as possible to achieve good performance. So far, synthesis of large-area graphene films by chemical vapor deposition of carbon precursors on metal substrates, especially on Cu, remains the main way to produce high-quality graphene, which has been significantly developed in the past 15 years.

Clinical application of a connection device consisting of a bag valve mask and nebulizer in first aid: Two case reports.

In clinical practice, several emergencies may threaten the life of patients, and these emergencies can be unpredictable and challenging. During the coronavirus disease 2019 pandemic, in January 2023, a patient developed respiratory distress caused by coronavirus, but was unable to access respiratory support due to shortages of medical resources, intensive care unit beds and ventilators. The medical staff quickly created a portable high-flow atomized oxygen therapy apparatus consisting of a simple breathing bag connected to a nebulizer to provide breathing support.

Single-cell atlas reveals the immunosuppressive microenvironment and Treg cells landscapes in recurrent Glioblastoma.

Patients diagnosed with glioblastoma (GBM) have the most aggressive tumor progression and lethal recurrence. Research on the immune microenvironment landscape of tumor and cerebrospinal fluid (CSF) is limited. At the single-cell level, we aim to reveal the recurrent immune microenvironment of GBM and the potential CSF biomarkers and compare tumor locations.

Study on a Pig Vocalization Classification Method Based on Multi-Feature Fusion.

To improve the classification of pig vocalization using vocal signals and improve recognition accuracy, a pig vocalization classification method based on multi-feature fusion is proposed in this study. With the typical vocalization of pigs in large-scale breeding houses as the research object, short-time energy, frequency centroid, formant frequency and first-order difference, and Mel frequency cepstral coefficient and first-order difference were extracted as the fusion features. These fusion features were improved using principal component analysis.

An Automated Centrifugal Microfluidic Platform for Efficient Multistep Blood Sample Preparation and Clean-Up towards Small Ion-Molecule Analysis.

Sample preparation for mass spectroscopy typically involves several liquid and solid phase clean-ups, extractions, and other unit operations, which are labour-intensive and error-prone. We demonstrate a centrifugal microfluidic platform that automates the whole blood sample's preparation and clean-up by combining traditional liquid-phase and multiple solid-phase extractions for applications in mass spectroscopy (MS)-based small molecule detection. Liquid phase extraction was performed using methanol to precipitate proteins in plasma separated from a blood sample under centrifugal force.

Crop diversification promotes soil aggregation and carbon accumulation in global agroecosystems: A meta-analysis.

Soil aggregation contributes to the stability of soil structure and the sequestration of soil organic carbon (SOC), making it an important indicator of soil health in agroecosystems. Crop diversification is considered a rational management practice for promoting sustainable agriculture. However, the complexity of cropping systems and crop species across different regions limits our comprehensive understanding of soil aggregation and associated carbon (C) content under crop diversification.

Soil microbial community parameters affected by microplastics and other plastic residues.

Introduction: The impact of plastics on terrestrial ecosystems is receiving increasing attention. Although of great importance to soil biogeochemical processes, how plastics influence soil microbes have yet to be systematically studied. The primary objectives of this study are to evaluate whether plastics lead to divergent responses of soil microbial community parameters, and explore the potential driving factors.

Recombinant human brain natriuretic peptide attenuates ischemic brain injury in mice by inhibiting oxidative stress and cell apoptosis via activation of PI3K/AKT/Nrf2/HO-1 pathway.

Ischemic stroke followed by cerebral artery occlusion is a main cause of chronic disability worldwide. Recombinant human brain natriuretic peptide (rhBNP) has been reported to alleviate sepsis-induced cognitive dysfunction and brain I/R injury. However, the function and molecular mechanisms of rhBNP in ischemic brain injury have not been clarified.

μPADs on Centrifugal Microfluidic Discs for Rapid Sample-to-Answer Salivary Diagnostics.

A fully integrated device for salivary detection with a sample-in-answer-out fashion is critical for noninvasive point-of-care testing (POCT), especially for the screening of contagious disease infection. Microfluidic paper-based analytical devices (μPADs) have demonstrated their huge potential in POCT due to their low cost and easy adaptation with other components. This study developed a generic POCT platform by integrating a centrifugal microfluidic disc with μPADs to realize sample-to-answer salivary diagnostics.

Hyperoside Attenuates Sepsis-Induced Acute Lung Injury (ALI) through Autophagy Regulation and Inflammation Suppression.

Background: Sepsis mortality and morbidity are aggravated by acute lung injury (ALI) or acute respiratory distress syndrome. Published studies have discovered that hyperoside (HYP) has an anti-inflammatory and therapeutic effect in many diseases. However, whether HYP treatment can attenuate sepsis-induced ALI is still obscure.

Characterization and application of a lytic jumbo phage ZPAH34 against multidrug-resistant .

is an emerging foodborne pathogen causing human gastroenteritis. species isolated from food such as seafood presented multidrug-resistance (MDR), raising serious concerns regarding food safety and public health. The use of phages to infect bacteria is a defense against drug-resistant pathogens.

Reducing spatial resolution increased net primary productivity prediction of terrestrial ecosystems: A Random Forest approach.

Net primary production (NPP) is a pivotal component of the terrestrial carbon dynamic, as it directly contributes to the sequestration of atmospheric carbon by vegetation. However, significant variations and uncertainties persist in both the total amount and spatiotemporal patterns of terrestrial NPP, primarily stemming from discrepancies among datasets, modeling approaches, and spatial resolutions. In order to assess the influence of different spatial resolutions on global NPP, we employed a random forest (RF) model using a global observational dataset to predict NPP at 0.

Fur functions as an activator of T6SS-mediated bacterial dominance and virulence in .

, a ubiquitous bacterium in aquatic habitats with broad host ranges, has earned the nickname of a 'Jack-of-all-trades'. However, there is still a limited understanding of the mechanism of how this bacterium fit the competition with other species in dynamic surroundings. The type VI secretion system (T6SS) is macromolecular machinery found in Gram-negative bacteria's cell envelope that is responsible for bacterial killing and/or pathogenicity toward different host cells.

Long-term plastic mulching decreases rhizoplane soil carbon sequestration by decreasing microbial anabolism.

Ridge-furrow with plastic mulching (RFPM) is a widely used agricultural practice in rain-fed farmlands. However, the impact of microbial related metabolism on soil organic carbon (SOC) is not fully understood. Amino sugar analysis, high-throughput sequencing, and high-throughput qPCR approaches are combined to investigate this topic, based on a long-term experiment.

Single cell RNA-seq reveals the CCL5/SDC1 receptor-ligand interaction between T cells and tumor cells in pancreatic cancer.

Pancreatic ductal adenocarcinoma (PDAC) is characterized by the complex tumor microenvironment (TME), consisting of an abundance of stromal cells. Among them, tumor infiltrating T cells play a pivotal role in tumor progress. To identify the full spectrum and developmental trajectory of T cells and their crosstalk with tumor cells in PDAC, we conducted scRNA-seq analysis based on multiple datasets from our institution and open databases.

Oral toxicity evaluation of genetically modified lactic acid bacteria in three generations of Sprague Dawley rats.

Genetic modification has great advantages in improving performance of bacteria, but its oral safety has not been systematically evaluated. In this study, the toxicity including the reproductive toxicity of two genetically modified bacteria engineered using food-grade vectors on three generations of rats (F0, F1 and F2) were studied. Sprague Dawley rats were administrated by gavage with corresponding parent and genetically modified Lactobacillus plantarum and Lactobacillus delbrueckii at 2.

Perianesthesia emergency repair of a cut endotracheal tube's inflatable tube: A case report.

Background: During the perianesthesia period, emergency situations threatening the life and safety of patients can occur at any time. When dealing with some emergencies, occasional confusion is inevitable.

Case Summary: This case report describes the rare situation wherein a surgeon inadvertently detached the inflatable tube of an endotracheal tube during a tonsillectomy, and positive pressure ventilation could not be provided.

Incentive policies for transboundary marine spatial planning: an evolutionary game theory-based analysis.

Marine spatial planning (MSP), which is a step-by-step approach for the ecosystem-based management (EBM) of global oceans, enables human activity and marine and coastal ecosystems conservation to occur simultaneously. It contributes to the implementation of the 2030 Agenda, particularly Sustainable Development Goal 14. However, the MSP, supposed as a transboundary policy-making process, is hampered by obstacles.

A time-space conversion method for material synthesis research.

Research on material synthesis is mostly performed through batch by batch testing with each corresponding to a set of parameters and a reaction time. Concurrent experiments that allow for multiple loadings throughout an inhomogeneous reaction zone provide a way to obtain high-throughput results. Here, a time-space conversion method is proposed.

Study of the spatio-temporal variation of environmental sustainability at national and provincial levels in China.

Environmental problems create a significant barrier for China in achieving its Sustainable Development Goals (SDGs). Assessing environmental sustainability is critical for China to meet the SDGs. Few studies, however, have looked into environmental sustainability in China.

Ecosystem service multifunctionality assessment and coupling coordination analysis with land use and land cover change in China's coastal zones.

Ecosystem services (ESs) have received widespread attention worldwide for their potential to solve sustainability issues. However, extensive land use and land cover change (LUCC) driven by human activities has raised concerns regarding its impacts on ESs, especially in coastal zones. More importantly, spatial-temporal changes, their coupling relationships with LUCC, and their underlying drivers have not been thoroughly analyzed.

Effects of double-dose statin therapy for the prevention of post-stroke epilepsy: A prospective clinical study.

Background: To determine treatment effects on the incidence of post-stroke epilepsy (PSE) using different doses of statin, a prospective hospital-based cohort study was designed to explore whether a double-dose statin treatment can better prevent the occurrence of PSE.

Methods: A total of 1152 patients with newly diagnosed ischemic stroke admitted to our hospital from March to August 2017 were selected, 1033 of whom were followed-up. Patients were divided into two treatment groups:(1) standard-dose (20 mg atorvastatin or 10 mg rosuvastatin,daily oral; 788 patients); and (2) double-dose (40 mg atorvastatin or 20 mg rosuvastatin, daily oral; 245 patients).