Fabrication of Functional 3D Nanoarchitectures via Atomic Layer Deposition on DNA Origami Crystals.

While DNA origami is a powerful bottom-up fabrication technique, the physical and chemical stability of DNA nanostructures is generally limited to aqueous buffer conditions. Wet chemical silicification can stabilize these structures but does not add further functionality. Here, we demonstrate a versatile three-dimensional (3D) nanofabrication technique to conformally coat micrometer-sized DNA origami crystals with functional metal oxides via atomic layer deposition (ALD).

Transcriptomic and proteomic analyses reveals the diverse venom composition of the spider Neoscona shillongensis.

Neoscona shillongensis (Araneidae), also known as the Shillong orb-weaving spider, is a species that constructs a typically shaped round web, is inactive during the daytime and comes out at dusk to forage, primarily preying on flying insects such as mosquitoes, flies, and moths. This spider serves as an important natural enemy of agricultural and forest pests. Neoscona shillongensis primarily uses its web to capture prey, but its venom also plays a crucial role in subduing and processing prey.

Drp1 knockdown aggravates obesity-induced cardiac dysfunction and remodeling.

Obesity is an independent risk factor for heart failure with preserved ejection fraction (HFpEF). Dynamin related protein 1 (Drp1) is a key regulator of mitochondrial morphology, bioenergetics and quality control. The role of endogenous Drp1 in obesity induced HFpEF remains largely unknown.

Alginate-polyacrylamide double-network hydrogel sensors: Biomass-based piezoresistive platform with liquid metal and convex array for enhanced sensitivity.

The hydrogel sensors with outstanding tensile strength, high sensitivity, broad sensing range and excellent stability are highly desired for flexible electronics. Here, a double network piezoresistive flexible hydrogel sensor equipped with a convex structure was fabricated using the template method, which consists of CMCNTs-PAM/SA-Ca convex array and ultra-high-conductivity liquid metal droplets (LMs). Such a novel hybrid architecture enables the prepared piezoresistive sensor to have high sensitivity (GF up to 32.

Time-performance relationship and the associated mechanism of tumor drug-efflux pump P-glycoprotein reversal induced by mild photothermal therapy.

Acquired multidrug resistance (MDR) has been the main cause leading to cancer therapy failure. Even though mild photothermal therapy (PTT) strategy has shown promise in overcoming tumor MDR, the detailed changing patterns of drug-resistant protein P-glycoprotein (P-gp) following photothermal treatment remains poorly understood. Herein, we utilized the iRGD-modified graphene oxide nanosheet (IPHG), a nanocarrier capable of tumor-targeted infiltration and photothermal conversion, to investigate the time-performance relationship and the associated mechanism of mild PTT-mediated P-gp regulation.

High-purity preparation and biophysical characterization of OX40-TC in lipid nanodiscs.

Tumor necrosis factor receptor superfamily member 4 (OX40) is essential for the activation and maintenance of T cell immune function because of its recognition and regulation in signal transduction, therefore understanding the structure and dynamics of the membrane protein OX40 in a near-native membrane environment is critical for elucidating its functions. However, efforts to prepare OX40 in a stable, functional form and reconstitute it into a membrane-mimicking lipid system face persistent challenges, limiting progress in its structural and functional characterization. Here, we developed an efficient method for the expression in E.

LPAR3 and COL8A1, as matrix stiffness-related biomarkers, promote nasopharyngeal carcinoma metastasis by triggering EMT and angiogenesis.

Matrix stiffness affects the progression of nasopharyngeal carcinoma, but the underlying mechanism is still unknown. Here, we demonstrated that nasopharyngeal carcinoma tissues with distant metastasis contain large collagen deposits and strong matrix stiffness. First, we performed RNA-seq analysis of nasopharyngeal carcinoma cells cultured on polyacrylamide hydrogel systems and found that LPAR3 and COL8A1 are potential matrix stiffness markers.

Effects of modified KGM on the gelling capability of fish protein: Novel insights into hooking effect.

This study investigated the effects of modified Konjac-Gluco-Mannan (KGM) on the gelling capability of fish protein through experimental and dynamic simulation methods. The addition of deacetylated KGM (DKGM) and carboxymethylated KGM (CKGM) significantly increased the gel strength of myosin gel by 0.69 times and 0.

The efficacy of ICIs rechallenge in advanced small cell lung cancer after progression from ICIs plus chemotherapy: A real-world study.

Background: Rechallenging immune checkpoint inhibitors(ICIs) after resistance to initial ICIs plus chemotherapy(chemo-ICIs) remains uncertain in advanced small cell lung cancer(SCLC).

Method: The study retrospectively enrolled advanced SCLC patients who received ICIs after developing resistance to chemo-ICIs during hospitalization at Hunan Cancer Hospital from January 2020 to November 2023. The clinical outcomes and prognosis factors for ICIs rechallenge were further analyzed.

A review of physiologically based pharmacokinetic modeling of renal drug disposition.

The human kidney is a critical organ for the elimination of numerous drugs and metabolites. The mechanisms of renal drug handling are manifold including unbound filtration, transporter-mediated active secretion, bidirectional passive diffusion, and occasionally active reabsorption and renal metabolism. These mechanisms collectively dictate the fate of drugs at various spatiotemporal points as drug molecules travel through the renal vasculature, tubules, and cells, posing a significant challenge in accurately describing and predicting renal drug disposition.

Evaluation of a novel ensemble model for preoperative ovarian cancer diagnosis: Clinical factors, O-RADS, and deep learning radiomics.

Background: Accurate early diagnosis of ovarian cancer is crucial. The objective of this research is to create a comprehensive model that merges clinical variables, O-RADS, and deep learning radiomics to support preoperative diagnosis and assess its efficacy for sonographers.

Materials And Methods: Data from two centers were used: Center 1 for training and internal validation, and Center 2 for external validation.

Achieving a High-Output Direct-Current Droplet Triboelectric Generator via Synergistic Effects of a Dual Switch and Electric Double Layer.

Droplet triboelectric generators (D-TENGs) have garnered significant attention for harvesting raindrop energy but face challenges such as low output performance and alternating current (AC) output. This study proposes a high-performance direct current (DC) D-TENG with a dual-switch (DS) structure (DS-DC-D-TENG) that synergizes dual-switch effects and electric double layers (EDL) to generate DC pulses. Remarkably, using 0.

Stacking the future of heterogeneous optoelectronics.

Integrated optoelectronics has emerged as the backbone of information exchange across all scales of modern digital infrastructure-from on-chip interconnects and board-level optical links to chassis-to-rack communications and transcontinental data center networks. It enables the seamless conversion of electrical signals to light and vice versa, overcoming the bandwidth and loss limitations of purely electronic systems. Today, its applications span critical junctions: Nanophotonic waveguides shuttle terabits of information per second between processor cores; silicon photonic transceivers mediate board-level communication with subpicojoule-per-bit energy efficiency; and fiber-optic arrays orchestrate exabyte-scale data flows between servers and across continents.

Unraveling enhanced liver regeneration in ALPPS: Integrating multi-omics profiling and in vivo CRISPR in mouse models.

Background: Postoperative liver failure due to insufficient liver cell quantity and function remains a major cause of mortality following surgery. Hence, additional investigation and elucidation are required concerning suitable surgeries for promoting in vivo regeneration.

Methods: We established the portal vein ligation (PVL) and associated liver partition and portal vein ligation for staged hepatectomy (ALPPS) mouse models to compare their in vivo regeneration capacity.

TaWUS-like-5D affects grain weight and filling by inhibiting the expression of sucrose and trehalose metabolism-related genes in wheat grain endosperm.

Plant-specific WUSCHEL-related homeobox (Wox) transcription factors (TFs) are crucial for plant growth and development. However, the molecular mechanism of Wox-mediated regulation of thousand kernel weight (TKW) in crops remains elusive. In this research, we identified a major TKW-associated quantitative trait locus (QTL) on wheat chromosome 5DS by performing a genome-wide association study (GWAS) of a Chinese wheat mini-core collection (MCC) in four environments combined by bulked segregant analysis (BSA) and bulked segregant RNA-sequencing (BSR-seq) of wheat grains exhibiting a wide range of TKWs.

Unobtrusive Sleep Health Assessment Using Impulse Radar: A Pilot Study in Older People.

Objective: Ultra-wideband (UWB) radar technology has emerged as a promising alternative for creating portable and cost-effective in-home monitoring devices. Although there exists good evidence supporting its effectiveness in sleep monitoring, previous studies predominantly focus on younger, healthy participants. This research evaluates the applicability of commercial impulse UWB radar for sleep monitoring in older people and people with neurodegenerative disorders (NDDs).

Large-Scale Plasma Proteomics to Profile Pathways and Prognosis of Chronic Pain.

While increasing peripheral mechanisms related to chronic pain, the plasma proteomics profile associated with it and its prognosis remains elusive. This study utilizes 2923 plasma proteins and chronic pain of 51 644 participants from UK Biobank and finds 474 proteins linked to chronic pain in six sites: head, neck or shoulder, back, stomach or abdominal, hip, and knee, with 11 proteins sharing across pain sites. The identified proteins are largely enriched in immune and metabolic pathways and highly expressed in tissues like lungs and small intestines.

Intrahepatic ductoplasty effectively corrects the ductal dilatation in Todani type IV-A choledochal cyst in children.

Primary ductal stricture and intrahepatic duct dilatation are characteristic features in Todani type IV-A choledochal cysts (CDC) and necessitate thorough evaluation and management during surgical treatment. This study aimed to present our experience with ductoplasties for type IV-A CDCs with primary ductal strictures. Between June 2015 and June 2022, 54 patients were reviewed.

Optimal timing for decompression in post-operative epidural hematoma: a retrospective analysis and treatment flowchart.

Purpose: Post-operative epidural hematoma is a rare but devastating complication of spine surgery. The importance of timely decompression for preserving neurologic function has been repeatedly stressed, but the acceptable timescale for decompression remains controversial. The purpose of this study was to propose a diagnosis and treatment flowchart for post-operative epidural hematoma.

Research on Monitoring Methods for Electrostatic Discharge Pulses in Spacecraft Dielectric Materials.

Space particle radiation induces charging and discharging phenomena in spacecraft dielectric materials, leading to electrostatic discharge (ESD) and electromagnetic pulses (EMP), which pose significant risks to spacecraft electronic systems by causing interference and potential damage. Accurate and timely monitoring of these phenomena, combined with a comprehensive understanding of their underlying mechanisms, is critical for developing effective protection strategies against satellite charging effects. Addressing in-orbit monitoring requirements, this study proposes the design of a compact sleeve monopole antenna.

Micro-Abrasive Air Jet Machining Technology for Fabrication of Helical Grooves on Bovine Bone.

Biological bone screws play an important role in fixing fractures and bone defects. The machining of helical grooves on xenogenic materials is a key part of fabricating biological bone screws. The fabrication of helical grooves on bovine bone using micro-abrasive air jets was investigated in this paper.

[Construction of a human anti-SARS-CoV-2 scFv library and identification of broad-spectrum neutralizing antibodies].

Objective To construct a library of human-derived anti-severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) single-chain variable fragments (scFv) and screen for broad-spectrum neutralizing antibodies to identify candidate molecules for the development of diagnostic and therapeutic agents. Methods Peripheral blood mononuclear cells (PBMCs) were isolated from the peripheral blood of patients who had recovered from novel coronavirus infection. Total RNA was extracted from these PBMCs and reverse transcribed into cDNA, which was used as a template for constructing a human anti-SARS-CoV-2 scFv library.

Ultrasound-Assisted H Transmitter Enables Hydrogen-Gene Therapy to Prevent Anesthesia/Surgery-Induced Cognitive Impairment.

Postoperative cognitive dysfunction (POCD) frequently occurs after surgery, resulting in extended hospitalizations, higher healthcare expenses, and potential long-term cognitive impairment. Despite its significant impact, effective preventive and therapeutic strategies for POCD are still lacking. Neuroinflammation plays a crucial role in the pathogenesis of POCD.

Mono-TKI and TKI Plus ICI in Unresectable Hepatocellular Carcinoma Progression on First-Line Treatment of Lenvatinib: A Real-World Study.

Background: Lenvatinib (LEN) is the recommended first-line therapy for unresectable hepatocellular carcinoma (uHCC), but resistance frequently develops, and limited data exist on second-line treatments. This study evaluated the efficacy and safety with a focus on the sorafenib (SOR) or regorafenib (REG)- based monotherapy or combination therapy in patients with uHCC after failure of first-line LEN.

Methods: Patients with first-line LEN failure between May 2018 and December 2023 were retrospectively collected.

Synthesis and evaluation of Calenduloside E against drug-resistant .

Calenduloside E (CE), also known as a triterpenoid saponin, has demonstrated diverse biological activities. This paper investigates the chemical synthesis of CE, focusing on the key step of glucuronidation of oleanolic acid benzylate. Among trichloroacetimidate, bromide, and thioglucuronide as glucuronyl donors, trichloroacetimidate proved most effective, with scandium(III) trifluoromethanesulfonate identified as the optimal promoter.