Publications by authors named "Yuta Tsujimori"
Article Synopsis
- Human milk is rich in carbohydrates, specifically human milk oligosaccharides (HMOs) and various types of glycans, but the specific profile of glycans had not been thoroughly studied until now.
- This study employed label-free chromatography-mass spectrometry (LC-MS) to analyze the variation in glycan composition and concentration in 200 samples of Japanese human milk from mothers 1-2 months postpartum.
- Findings showed that the presence of fucose linked to galactose affected the abundance of certain glycans and HMOs, with secretor mothers producing more of these compounds compared to nonsecretors, although the overall profile of glycans remained unchanged.
Background: The effects of dietary patterns on health outcome of lactating women remain unclear.
Objectives: To describe the dietary patterns of lactating Japanese women and explore the association between dietary patterns and their general health.
Methods: This study included 1096 lactating women from the Japanese Human Milk Study Cohort.
Article Synopsis
- The Japanese Human Milk Study aims to explore how various factors like maternal health and lifestyle impact breastfeeding practices and the composition of human milk to understand their effects on infant and child development.
- A total of 1122 lactating women participated in the study, showing a relatively healthy sample group with a mean age of 31.2 years and a high percentage with college education.
- Future plans include ongoing monthly milk sample collection and health surveys to analyze changes in milk composition and the influence of lifestyle factors on maternal and child health until the children are five years old.*
Article Synopsis
- The study examined the link between parenting stress and the type of breastfeeding (exclusive vs. partial) among 1210 mothers in Japan from 2014 to 2019.
- Results showed higher parenting stress related to childcare exhaustion and worries about child development in mothers who partially breastfed compared to those who breastfed exclusively at two months, but the difference disappeared by six months.
- The findings suggest that addressing parenting stress in the early postpartum period could help support longer exclusive breastfeeding durations.
Background: Developing a simple quantitative tool for mastitis diagnosis is essential. The Ion-Selective Electrode for sodium has been reported to reliably measure sodium concentrations in human milk.
Research Aim: To determine whether an Ion-Selective Electrode measurement of sodium:potassium ratios could serve as a diagnostic tool for mastitis and, if so, to determine the diagnostic cut-off value.
Article Synopsis
- A five-year cohort study aims to investigate the relationship between maternal health, nutrition, socioeconomic factors, and the composition of human milk, and how these elements may influence the growth and development of infants and children.
- The study includes 1,210 Japanese mothers with singleton pregnancies, gathering data from self-administered questionnaires and diet history assessments to analyze various health and lifestyle factors.
- Human milk samples will be collected every two months in the first year post-birth to analyze macronutrient levels, providing valuable insights into the factors that may affect both milk composition and child development.
Article Synopsis
- Free glycans (FNGs) are found in many growing plants and play a role in the breakdown of dysfunctional glycoproteins, with a specific focus on plant complex-type FNGs (PCT-FNGs).
- This study highlights the presence of PCT-FNGs in the xylem sap of tomato plant stems, revealing an area that has not previously been analyzed.
- Various chemical terminology and methods, such as RP-HPLC and SF-HPLC, are referenced to describe the types and structures of these complex glycans.
In a previous study, we molecular-characterized a tomato (Solanum lycopersicum) α1, 3/4-fucosidase (α-Fuc'ase Sl-1) encoded in a tomato gene (Solyc03g006980), indicating that α-Fuc'ase Sl-1 is involved in the turnover of Lea epitope-containing N-glycans. In this study, we have characterized another tomato gene (Solyc11g069010) encoding α1, 3/4-fucosidase (α-Fuc'ase Sl-2), which is also active toward the complex type N-glycans containing Lea epitope(s). The baculovirus-insect cell expression system was used to express that α-Fuc'ase Sl-2 with anti-FLAG tag, and the expression product (rFuc'ase Sl-2), was found as a 65 kDa protein using SDS-PAGE and has an optimum pH of around 5.
View Article and Find Full Text PDF