Combined Light and Thermal Stimulation of Bone Marrow Stem Cells.

The purpose of this study is to achieve a significant increase in the proliferative activity of mesenchymal stem cells (MSCs) of the bone marrow (BM) at early passages after laser exposure to a suspension of these cells and to estimate the effect of light and heat components of laser radiation on the proliferation of BM MSCs. The studies were performed on rats BM MSCs. MSC suspension was placed into the wells and heated by using laser radiation (980 nm wavelength) or a water bath at 70 °C providing similar temperature dynamics.

Laser Bioprinting with Cell Spheroids: Accurate and Gentle.

Laser printing with cell spheroids can become a promising approach in tissue engineering and regenerative medicine. However, the use of standard laser bioprinters for this purpose is not optimal as they are optimized for transferring smaller objects, such as cells and microorganisms. The use of standard laser systems and protocols for the transfer of cell spheroids leads either to their destruction or to a significant deterioration in the quality of bioprinting.

The origin of loud claps during endovenous laser treatments.

Optoacoustic and ultrasound methods have shown that the loud "claps" perceived by patients and medical staff during endovenous laser ablation (EVLA) are caused by volumetric blood boiling when large vapor-gas bubbles appear and collapse under the action of laser radiation, which is well absorbed in water. Acoustic effects when using lasers in the near infrared range (1.94, 1.

Laser Thermo-Photobiomodulation of Bone Marrow Mesenchymal Stem Cells.

We studied the effect of laser radiation of moderate intensity with a wavelength of 970 nm on the efficiency of colony formation of rat bone marrow mesenchymal stem cells (MSC) in vitro. In this case, photobimodulation and thermal heating of MSC occur simultaneously. This combined laser treatment allows increasing the number of colonies by 6 times in comparison with the control and by more than 3 times in comparison with thermal heating alone.

Production, Mechanical and Functional Properties of Long-Length TiNiHf Rods with High-Temperature Shape Memory Effect.

In the present work, the possibility of manufacturing long-length TiNiHf rods with a lowered Hf content and a high-temperature shape memory effect in the range of 120-160 °C was studied. Initial ingots with 1.5, 3.

Evolution of Structure and Properties of Nickel-Enriched NiTi Shape Memory Alloy Subjected to Bi-Axial Deformation.

The effect of a promising method of performing a thermomechanical treatment which provides the nanocrystalline structure formation in bulk NiTi shape memory alloy samples and a corresponding improvement to their properties was studied in the present work. The bi-axial severe plastic deformation of Ti-50.7at.

Effect of Rotary Swaging on the Structure, Mechanical Characteristics and Aging Behavior of Cu-0.5%Cr-0.08%Zr Alloy.

A study of the effect of rotary swaging (RS) on the microstructure and properties of the pre-extruded and pre-quenched Cu-0.5%Cr-0.08%Zr alloy was performed.

Laser-assisted bioprinting of microorganisms with hydrogel microdroplets: peculiarities of Ascomycota and Basidiomycota yeast transfer.

Laser-assisted bioprinting of microbial cells by hydrogel microdroplets is a rapidly developing and promising field that can contribute to solving a number of issues in microbiology and biotechnology. To date, most research on the use of laser bioprinting for microorganism manipulation and sorting has focused on prokaryotes; the bioprinting of eukaryotic microorganisms is much less understood. The use of hydrogel allows solving two fundamental problems: creating comfortable environments for living microorganisms and imparting the necessary rheological properties of the gel for the stable transfer of microdroplets of a preset size.

Photobiomodulation in 3D tissue engineering.

Significance: The method of photobiomodulation (PBM) has been used in medicine for a long time to promote anti-inflammation and pain-resolving processes in different organs and tissues. PBM triggers numerous cellular pathways including stimulation of the mitochondrial respiratory chain, alteration of the cytoskeleton, cell death prevention, increasing proliferative activity, and directing cell differentiation. The most effective wavelengths for PBM are found within the optical window (750 to 1100 nm), in which light can permeate tissues and other water-containing structures to depths of up to a few cm.

Theoretical and Experimental Assay of Shock Experienced by Yeast Cells during Laser Bioprinting.

Laser-induced forward transfer (LIFT) is a useful technique for bioprinting using gel-embedded cells. However, little is known about the stresses experienced by cells during LIFT. This paper theoretically and experimentally explores the levels of laser pulse irradiation and pulsed heating experienced by yeast cells during LIFT.

Changes in the Number of Mesenchymal Stem Cells in Bone Marrow at Different Periods after In Vivo Exposure of the Bone Marrow to Local Infrared Laser Radiation.

We determined optimal parameters of bone marrow (BM) irradiation in vivo for rapid increase in the number of mesenchymal stem cells (MSC) at the initial stages of the culturing without changing the karyotype, polyploidy, which are observed at higher passages. Such an increase is necessary to achieve the required number of cells at the initial passages for subsequent transplantation into the body. It was shown that after irradiation with λ=0.

Low-Intensity Femtosecond Radiation Activates the Natural Defenses of Mice in vivo.

The possibility of induction of cytogenetic damage in the bone marrow, changes in the cellularity of lymphoid organs and blood composition in mice irradiated with low-intensity femtosecond laser radiation at a power flux density of 5.1, 10.4, and 52 mJ/cm (0.

Metal Nanoparticles in Laser Bioprinting.

Laser bioprinting is a promising method for applications in biotechnology, tissue engineering, and regenerative medicine. It is based on a microdroplet transfer from a donor slide induced by laser pulse heating of a thin metal absorption film covered with a layer of hydrogel containing living cells (bioink). Due to the presence of the metal absorption layer, some debris in the form of metal nanoparticles is printed together with bioink microdroplets.

Optical and thermal fields induced in the bone marrow by external laser irradiation.

In regenerative medicine, the problem of growing mesenchymal stem cells from the bone marrow often arises. In such cases is important that the number of initial cells was large enough and their proliferative activity was high. We believe that this problem can be solved by short-term heating of local areas of the bone marrow in vivo with laser radiation.

Two Approaches to the Laser-Induced Formation of Au/Ag Bimetallic Nanoparticles in Supercritical Carbon Dioxide.

Two approaches are proposed for the synthesis of bimetallic Au/Ag nanoparticles, using the pulsed laser ablation of a target consisting of gold and silver plates in a medium of supercritical carbon dioxide. The differences between the two approaches related to the field of "green chemistry" are in the use of different geometric configurations and different laser sources when carrying out the experiments. In the first configuration, the Ag and Au targets are placed side-by-side vertically on the side wall of a high-pressure reactor and the ablation of the target plates occurs alternately with a stationary "wide" horizontal beam with a laser pulse repetition rate of 50 Hz.

Endovenous laser coagulation: asymmetrical heat transfer and coagulation (modeling in blood plasma).

The objective of this study was to describe the dynamics of blood plasma heating and coagulation processes carried out by continuous laser radiation with wavelengths 1.55 and 1.94 μm through bare-tip fibers and fibers with radial output (radial fibers) used for endovenous laser coagulation (EVLC).

Synthesis of Supported Heterogeneous Catalysts by Laser Ablation of Metallic Palladium in Supercritical Carbon Dioxide Medium.

To obtain a supported heterogeneous catalyst, laser ablation of metallic palladium in supercritical carbon dioxide was performed in the presence of a carrier, microparticles of γ-alumina. The influence of the ablation process conditions-including supercritical fluid density, ablation, mixing time of the mixture, and laser wavelength-on the completeness and efficiency of the deposition of palladium particles on the surface of the carrier was studied. The obtained composites were investigated by scanning and transmission electron microscopy using energy dispersive spectroscopy.

Endovenous laser coagulation: asymmetrical heat transfer (modeling in water).

The objective of this study was to describe the dynamics of water heating carried out by continuous laser radiation with wavelengths 1.47, 1.55, and 1.

Laser-induced Forward Transfer Hydrogel Printing: A Defined Route for Highly Controlled Process.

Laser-induced forward transfer is a versatile, non-contact, and nozzle-free printing technique which has demonstrated high potential for different printing applications with high resolution. In this article, three most widely used hydrogels in bioprinting (2% hyaluronic acid sodium salt, 1% methylcellulose, and 1% sodium alginate) were used to study laser printing processes. For this purpose, the authors applied a laser system based on a pulsed infrared laser (1064 nm wavelength, 8 ns pulse duration, 1 - 5 J/cm laser fluence, and 30 μm laser spot size).

Effects of photobiomodulation in relation to HeLa Kyoto tumor cells exposed to ionizing radiation.

The aim of the work was studying the effects of photobiomodulation of a red spectrum in doses of less than 1 J/cm in combination with gamma-irradiation to Hela Kyoto cells. Tumor cells were irradiated with 640 nm LED at different energy densities before and after to gamma-irradiation. Cells viability was determined 24 h after exposure for each gamma-irradiation dose and each PBM mode.

Effects of Photobiomodulation on Daphnia magna Straus and their Sensitivity to Toxicant.

This paper deals with the effect of photobiomodulation (PBM) on Daphnia magna S. and their sensitivity to cadmium sulfate, a known high toxic pollutant. In a first series of experiments, the effect of different He-Ne laser fluences irradiation (range 0.

Digging deeper: structural background of PEGylated fibrin gels in cell migration and lumenogenesis.

Fibrin is a well-known tool in tissue engineering, but the structure of its modifications created to improve its properties remains undiscussed despite its importance, in designing biomaterials that ensure cell migration and lumenogenesis. We sought to uncover the structural aspects of PEGylated fibrin hydrogels shown to contribute to angiogenesis. The analysis of the small-angle X-ray scattering (SAXS) data and modeling revealed that the PEGylation of fibrinogen led to the formation of oligomeric species, which are larger at a higher PEG : fibrinogen molar ratio.