Finite Element Modeling for Biomechanical Comparisons of Multilevel Transforaminal, Posterior, and Lateral Lumbar Approaches to Interbody Fusion Augmented with Posterior Instrumentation.

Objective: Verifying the intervertebral stability of each intervertebral fusion procedure, including transforaminal, posterior, and lateral lumbar interbody fusion (TLIF, PLIF, and LLIF, respectively), and the ratio of stress on the rods and pedicle screws during initial fixation may help select a fixation procedure that reduces the risk of mechanical complications, including rod fracture and screw loosening. Thus, we aimed to assess whether these procedures could prevent mechanical complications.

Methods: Using the finite element method (FEM), we designed 4 surgical models constructed from L2-5 as follows: posterior lumbar fusion (PLF), TLIF, PLIF, and LLIF models.

Evaluation of the Capacity to Produce Histamine by Histamine-Producing Bacteria during Storage at 10℃.

Histamine is produced from histidine using histidine decarboxylase of histamine-producing bacteria. However, associated histamine food poisoning demands microbiological controls. Furthermore, studies reported that histamine production by histamine-producing bacteria is affected by temperature.

Co-administration of a plasmid encoding CD40 or CD63 enhances the immune responses to a DNA vaccine against bovine viral diarrhea virus in mice.

Bovine viral diarrhea virus (BVDV) causes substantial economic losses in the livestock industry worldwide. Plasmids encoding the BVDV E2 protein are potential DNA vaccines against BVDV, but their immunogenicity has been insufficient. Here, we investigated the adjuvant effect of CD40 and CD63 plasmids on the immune responses to a BVDV E2 DNA vaccine in mice.

Dynamic ionic radius of alkali metal ions in aqueous solution: a pulsed-field gradient NMR study.

The dynamic behavior of alkali metal ions, Li, Na, K, Rb and Cs in aqueous solutions is one of the most important topics in solution chemistry. Since these alkali metals contain nuclear magnetic resonance (NMR) active nuclei, it is possible to directly measure the diffusion constants of the alkali metal ions using the pulsed field gradient (PFG) NMR method. In this paper, the Li, Na, Rb, Cs and H resonances are observed for diffusion constants in aqueous solution and the solvent HO.

Comparison of Hybrid Posterior Fixation and Conventional Open Posterior Fixation Combined with Multilevel Lateral Lumbar Interbody Fusion for Adult Spinal Deformity.

We compared radiological and clinical outcomes between multilevel lateral lumbar interbody fusion (LLIF) + hybrid posterior fixation (PF) and multilevel LLIF + conventional open PF in patients with adult spinal deformity (ASD). Patients who underwent minimally invasive surgery for ASD in a single institution between 2014 and 2018 were retrospectively reviewed. Fifty-six patients (hybrid PF, 30; open PF, 26) who underwent ASD correction surgery were enrolled between 2014 and 2018.

Characterization of microRNA expression in B cells derived from Japanese black cattle naturally infected with bovine leukemia virus by deep sequencing.

Bovine leukemia virus (BLV) is the causative agent of enzootic bovine leukosis (EBL), a malignant B cell lymphoma. However, the mechanisms of BLV-associated lymphomagenesis remain poorly understood. Here, after deep sequencing, we performed comparative analyses of B cell microRNAs (miRNAs) in cattle infected with BLV and those without BLV.

Electrochemical Crystal Growth of Titanium Oxyfluorides-A Strategy for Development of Electron-Doped Materials.

We report on the growth of single crystals of an electron-doped titanium oxyfluoride, LiTi(O,F), employing high-temperature electrolysis of TiO with a eutectic LiMoO-LiF melt. Greenish octahedral-shaped crystals (∼30 μm in size) with a cubic rocksalt-type structure were successfully obtained by precisely tuning the applied voltage. The temperature-dependent magnetic susceptibility data revealed a paramagnetic behavior at low temperatures, ensuring the presence of Ti ions (mean valence number of +3.

Effectiveness of on-farm continuous flow high-temperature short-time pasteurization for inactivation of bovine leukemia virus in milk.

The effectiveness of on-farm continuous flow high-temperature short-time (HTST) pasteurization (i.e., 72°C for 15 s) for the inactivation of bovine leukemia virus (BLV) in milk was investigated with a sheep bioassay.

Nationwide Survey of Infectious Bronchitis Virus on Layer Farms in Japan During 2015.

After accumulating data through a nationwide survey, we characterized the recent prevalences and geographic distributions of various genotypes of infectious bronchitis virus (IBV) on layer farms in Japan. Reverse transcription PCR analysis of fecal samples revealed the presence of the IBV nucleoprotein (N) gene on approximately 30% of the farms surveyed. N-gene detection rates were higher in the Chugoku and Kyushu regions than in the remaining surveyed regions.

Quantification of metal-induced susceptibility artifacts associated with ultrahigh-field magnetic resonance imaging of spinal implants.

Reports on spinal-implant metallic artifacts in 7-T magnetic resonance imaging (MRI) are lacking. Thus, we investigated the magnitude of metal artifacts derived from spinal implants in 7-T MRI and analyzed the differences obtained with spinal rods manufactured from pure titanium, titanium alloy, and cobalt-chrome (5.5-mm and 6.

Efficient luminescent properties and cation recognition ability of heavy group 13 element complexes of NO- and NO-type dipyrrins.

The first mononuclear 1 : 1 complexes of heavy group 13 elements (Ga and In) and NO/NO-type dipyrrins were synthesized and characterized. The NO-type complexes showed efficient luminescent properties even in polar solvents. The NO-type complexes exhibited fluorometric responses to alkaline earth metal ions.

Two-step template method for synthesis of axis-length-controlled porphyrin-containing hollow structures.

π-Conjugated porphyrin-containing hollow structures with defined axis-length were successfully synthesized by a two-step template method, i.e., template-assisted cyclization and oligomerization.

Evaluation of the serum ionic fluoride concentration as a biomarker of bone metabolism post-spinal fusion surgery.

Background: Bone union after spinal fusion surgery with instrumentation has been determined only with imaging studies. We evaluated the usefulness of the serum ionic fluoride (SIF) concentration as a biomarker of the bone union status.

Methods: We enrolled 25 patients who underwent spinal surgery in our institution, and we divided patients into three groups with and without instrumentation (G1, G2, and G3).

Hetero Face-to-Face Porphyrin Array with Cooperative Effects of Coordination and Host-Guest Complexation.

We successfully synthesized a hetero face-to-face porphyrin array composed of ZnTPP and RuTPP(DABCO) (TPP: 5, 10, 15, 20-tetraphenylporphyrin, DABCO: 1,4-diazabi-cyclo[2.2.2]octane) in 2:1 molar ratio.

Dynamics of different bacterial communities are capable of generating sustainable electricity from microbial fuel cells with organic waste.

The relationship between the bacterial communities in anolyte and anode biofilms and the electrochemical properties of microbial fuel cells (MFCs) was investigated when a complex organic waste-decomposing solution was continuously supplied to MFCs as an electron donor. The current density increased gradually and was maintained at approximately 100 to 150 mA m(-2). Polarization curve analyses revealed that the maximum power density was 7.

Differential epigenetic regulation of BDNF and NT-3 genes by trichostatin A and 5-aza-2'-deoxycytidine in Neuro-2a cells.

To understand epigenetic regulation of neurotrophins in Neuro-2a mouse neuroblastoma cells, we investigated the alteration of CpG methylation of brain-derived neurotrophic factor (BDNF) promoter I and neurotrophin-3 (NT-3) promoter IB and that of histone modification in Neuro-2a cells. Bisulfite genomic sequencing showed that the CpG sites of BDNF promoter I were methylated in non-treated Neuro-2a cells and demethylated following 5-aza-2'-deoxycytidine (5-aza-dC) treatment. In contrast, methylation status of the NT-3 promoter IB did not change by 5-aza-dC treatment in Neuro-2a cells.

Robust stimulation of TrkB induces delayed increases in BDNF and Arc mRNA expressions in cultured rat cortical neurons via distinct mechanisms.

In cultures of rat cortical neurons, we found that stimulation of tyrosine receptor kinase B (TrkB) with brain-derived neurotrophic factor (BDNF) induced a biphasic expression of BDNF exon IV-IX mRNA, which became obvious 1-3 h (primary induction) and 24-72 h (delayed induction) after the stimulation, and characterized the delayed induction in relation to the mRNA expression of activity-regulated cytoskeleton-associated protein (Arc). Withdrawal of BDNF from the medium after stimulation for 3 h allowed the delayed induction, which was caused at the transcriptional level and dependent upon the initial contact between exogenously added BDNF and TrkB, the effect of which was time- and dose-dependent. The primary induction was controlled by the extracellular signal-regulated kinase/mitogen-activated protein kinase (ERK/MAPK) whereas the secondary induction by the calcium (Ca(2+)) signaling pathway.