Publications by authors named "Yusuf P Tan"
Article Synopsis
- NMDA enhances spontaneous GABA release in developing cerebellar molecular layer interneurons, potentially through presynaptic NMDA receptors (preNMDARs) or an indirect pathway involving somato-dendritic NMDARs.
- Experiments using Ca(2+) imaging and electrophysiology showed that activating NMDA or glutamate at specific axonal spots triggered significant but localized Ca(2+) transients, sensitive to the location of the stimulation.
- The findings indicate that preNMDARs likely exist in developing MLIs and play a critical role in increasing GABA release via a Ca(2+)-induced release mechanism from presynaptic stores, which may be essential for synaptic plasticity.
The role of the axonal membrane compartment in synaptic integration is usually neglected. We show here that in interneurons of the cerebellar molecular layer, where dendrites are so short that the somatodendritic domain can be considered isopotential, the axonal membrane contributes a significant part of the cell input capacitance. We examine the impact of axonal membrane on synaptic integration by cutting the axon with two-photon illumination.
View Article and Find Full Text PDFThe existence of spontaneous calcium transients (SCaTs) dependent on intracellular store activation has been reported in putative axonal terminals of cerebellar basket interneurons. We used the two-photon imaging technique to optically identify basket terminals in acute cerebellar slices of young rats (11-16 d old) and study the properties of SCaTs unambiguously localized in these regions. The whole-cell recording configuration and preloading technique were alternatively used to load the calcium-dependent dye in the interneuron and compare SCaTs with action potential evoked calcium transients.
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