Biofiltration system is a widely used stormwater treatment option that is effective in removing heavy metals. The concentration and distribution of heavy metal fractions in biofiltration filter media, as well as the microbiota composition affected by the design parameters, are relatively novel concepts that require further research. A laboratory-scale column study was conducted to investigate the microbial community and the fractionation of heavy metals (Pb, Cu, Cr, and Cd) extracted from filter media samples, subjected to the presence of vegetation, submerged zone (SZ), and major environmental parameters (pH, water content).
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