Functional intestinal monolayers from organoids derived from human iPS cells for drug discovery research.

Background: Human induced pluripotent stem (iPS) cell-derived enterocyte-like cells (ELCs) are expected to be useful for evaluating the intestinal absorption and metabolism of orally administered drugs. However, it is difficult to generate large amounts of ELCs with high quality because they cannot proliferate and be passaged.

Methods: To solve the issue above, we have established intestinal organoids from ELCs generated using our protocol.