Publications by authors named "Yusaku Ooki"
Article Synopsis
- ATF5 is a transcription factor crucial for the differentiation and survival of sensory neurons in mouse olfactory organs, yet its specific target genes were previously unknown.
- Chromatin immunoprecipitation-quantitative polymerase chain reaction (ChIP-qPCR) experiments identified Nhlh1 as a novel target gene for ATF5, showing that ATF5 binds to its enhancer region and influences its expression.
- The study suggests that ATF5, along with the co-factor Lhx2, plays a vital role in regulating Nhlh1 to promote the development of olfactory and vomeronasal sensory neurons.
Activating transcription factor 5 (ATF5) is a stress-responsive transcription factor that belongs to the cAMP response element-binding protein (CREB)/ATF family, and is essential for the differentiation and survival of sensory neurons in murine olfactory organs. However, the study of associated proteins and target genes for ATF5 has been hampered due to the limited availability of immunoprecipitation-grade ATF5 antibodies. To overcome this issue, we generated hemagglutinin (HA)-tag knock-in mice for ATF5 using CRISPR/Cas9-mediated genome editing with one-step electroporation in oviducts (i-GONAD).
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