Contrast-enhanced optical microscopy using a planar photonic substrate and dark-field illumination.

Visualization of low-index dielectric nanoparticles and biological nanostructures is challenging under a traditional optical microscope. In this work, we propose a planar photonic substrate to enhance the extremely weak scattering signal and improve the contrast in imaging low-index samples under a dark-field illumination (DFI) optical microscope. Specifically, a planar photonic substrate is prepared by depositing a multilayer optical film on a silica substrate with the maximum electric field intensity distributed on the substrate surface.

Microsphere-assisted super-resolution polarized light microscopy for polarization-sensitive materials.

Polarization sensitivity is a fundamental phenomenon observed in nature, and its application is vital for advancing scientific discoveries. Here, we present a microsphere-assisted polarized light microscopy method that enables high-quality, label-free super-resolution imaging of polarization-sensitive materials. We investigated the imaging performance of this method using various samples, including colloidal crystals and grating structures.

Performance of microsphere-assisted imaging in bright-field and dark-field microscopy.

In this work, we study the imaging performance of microsphere-assisted microscopy (MAM) using microspheres with different refractive indices and immersion conditions under both bright-field illumination (BFI) and dark-field illumination (DFI). The experimental results show that the position of the photonic nanojet of the microsphere plays an important role in MAM imaging. The contrast in imaging is affected by the reflection from the microsphere, the background signal without the microsphere, and the electric field on the substrate surface.

Assessment of health risks associated with prediction of vegetable inorganic arsenic concentrations given different soil properties.

Inorganic arsenic (iAs) is a carcinogen. Vegetables such as water spinach (Ipomoea aquatica Forssk.) and amaranth (Amaranthus mangostanus L.

Super-resolution imaging based on cascaded microsphere compound lenses.

In this paper, a cascaded microsphere compound lens (CMCL) is introduced, in which a 20-µm-diameter barium titanate glass (BTG) primary microsphere and a 250-nm-diameter or 200-nm-diameter polystyrene (PS) secondary microsphere array constitute CMCL1 and CMCL2, respectively. The field of view (FOV) depends on the size of the BTG microsphere, while the waist of the photon nanojet (PNJ) can be adjusted by the size of the PS microsphere. The narrower the waist of the PNJ, the higher the imaging resolution.

Microsphere-assisted dark-field microscopy based on a fully immersed low refractive index microsphere.

Here we find that a fully immersed low refractive index SiO microsphere (or a microcylinder, a yeast cell) can clearly distinguish a sample with sub-diffraction features in dark-field illumination mode. The resolvable area of the sample by microsphere-assisted microscopy (MAM) is composed of two regions. One region locates below the microsphere, and a virtual image of this part of the sample is formed by the microsphere first and then the virtual image is received by the microscope.

Surface plasmon-enhanced dark-field microsphere-assisted microscopy.

We present for the first time a surface plasmon-enhanced dark-field microsphere-assisted microscopy in imaging both low-contrast dielectric objects and metallic ones. We demonstrate, using an Al patch array as the substrate, the resolution and contrast in imaging low-contrast dielectric objects are improved compared to that of the metal plate substrate and a glass slide in dark-field microscopy (DFM). 365-nm-diameter hexagonally arranged SiO nanodots assembled on the three substrates can be resolved, with the contrast varied from 0.

Super-resolution imaging of plasmonic nanostructures by microsphere-assisted microscopy.

We fabricate both triangularly and circularly shaped Au, Ag, and Cr nanoparticle arrays and observe the imaging properties of these plasmonic nanostructures by glass (BTG) microsphere-assisted microscopy. We experimentally find that the resolution of triangularly shaped Ag nanoparticle arrays is higher than that of Au and Cr ones, and a gap resolution of ∼/7.7 is demonstrated for the circularly shaped Au, Ag, and Cr nanostructures.

The Destruction of the Anaerobic Environment Caused by Rumen Fistula Surgery Leads to Differences in the Rumen Microbial Diversity and Function of Sheep.

This experiment was to study the impact of rumen fistula surgery on the rumen microbios in sheep. Six male adult sheep (48.8 ± 0.

Changes in the Fermentation and Bacterial Community by Artificial Saliva pH in RUSITEC System.

The purpose of the study was to assess the artificial saliva (AS) pH on ruminal fermentation and rumen bacteria community in the rumen simulation technique (RUSITEC) system. The experiment was performed in two treatments (low AS pH vs. high AS pH) with four replicates.

Association Between Menstrual Patterns and Adverse Pregnancy Outcomes in Patients With Polycystic Ovary Syndrome.

Objective: To investigate the results of fertilization among polycystic ovary syndrome (PCOS) patients using the long-acting long protocol regarding the relationship between menstrual patterns and adverse pregnancy outcomes.

Design: Retrospective cohort study.

Setting: University-affiliated reproductive medical center.

Morphological, Physiological, and Molecular Responses of Sweetly Fragrant During the Floral Transition Stage Induced by Short-Day Photoperiod.

Photoperiod-regulated floral transition is vital to the flowering plant. "Xiangfei" is a flowering ornamental plant with high development potential economically and is a short-day woody perennial. However, the genetic regulation of short-day-induced floral transition in is unclear.

Application of a Nomogram for Predicting the Risk of Subchorionic Hematoma in Early Pregnancy With In Vitro Fertilization-Embryo Transfer/Frozen Embryo Transfer.

Background: Subchorionic hematoma (SCH) is common in early pregnancy achieved by fertilization-embryo transfer/frozen embryo transfer (IVF-ET/FET), and is associated with adverse obstetric outcomes. However, there are no methods known to accurately predict the occurrence of SCH.

Objective: To establish a nomogram prediction model for predicting the risk of SCH in early pregnancy with IVF-ET/FET and to analyze pregnancy outcomes of patients with SCH.

Construction of a ceRNA-based lncRNA-mRNA network to identify functional lncRNAs in polycystic ovarian syndrome.

Polycystic ovary syndrome (PCOS) is a prevalent endocrine and metabolic disorder in women of childbearing age. Recent studies suggest important roles for lncRNAs in PCOS development. Based on the hypothesis that lncRNAs are able to regulate mRNA functions by competitive binding to shared miRNAs, the present work sought to construct a PCOS-related lncRNA-mRNA network (PCLMN) to identify key lncRNAs with dysregulated expression and potential prognostic and therapeutic relevance.

Association between hyperandrogenism and adverse pregnancy outcomes in patients with different polycystic ovary syndrome phenotypes undergoing fertilization/intracytoplasmic sperm injection: a systematic review and meta-analysis.

Objective: To study the association between hyperandrogenism (HA) and adverse pregnancy outcomes in patients with different polycystic ovary syndrome phenotypes undergoing fertilization (IVF)/intracytoplasmic sperm injection (ICSI).

Methods: We reviewed all eligible articles published up to October 2020 after searching in PubMed, Embase, Cochrane Library, Web of Science, Wanfang Data, and CNKI databases. The primary outcomes were the clinical pregnancy rate (CPR), miscarriage rate (MR), and live birth rate (LBR), whereas the secondary outcomes were the number of retrieved oocytes and endometrial thickness.

Effect and Relationship of Seasons on the High Risk of Ovarian Hyperstimulation Syndrome After Oocyte Retrieval in Patients With Polycystic Ovary Syndrome.

Objective: To investigate the effect of seasons on the incidence of high risk of ovarian hyperstimulation syndrome (OHSS) after in oocyte retrieval in patients with polycystic ovarian syndrome (PCOS) and to establish a nomogram to predict the risk of OHSS.

Design: Single-center, retrospective study.

Setting: University-affiliated reproductive medicine center.

Excitatory transmission from ventral pallidum to lateral habenula mediates depression.

Lateral habenula (LHb) is a key brain structure for mediating behavioural responses to aversive stimuli. It receives presynaptic inputs from ventral pallidum (VP) which relates to reward, motivation and hedonics. This study investigated the role of glutamatergic VP-LH projection in negative emotions and depression-like behaviour.

fertilization outcome in women with endometrial tuberculosis and tubal tuberculosis.

To evaluate fertilization (IVF) or intracytoplasmic sperm injection (ICSI) outcome in women with genital tuberculosis (GTB). One hundred and fifty-five women with genital tuberculosis constituted the study group (Group A), consisting of 25 patients with endometrial tuberculosis (Group A1) and 130 patients with tubal tuberculosis (Group A2). Women with non-tuberculous tubal infertility were matched by age and study period served as controls (Group B).

Construction and Recognition of Functional Brain Network Model Based on Depression.

In order to make a deep analysis of depression, the construction and recognition of functional brain network model based on depression is mainly studied. Firstly, the relevant information of the subjects is introduced in turn. The data of fMRI (functional magnetic resonance imaging) are pretreated, static and dynamic functional connections are analyzed, statistical analysis and testing are carried out, and then the results are analyzed and discussed.

Early metabolic characterization of brain tissues after whole body radiation based on gas chromatography-mass spectrometry in a rat model.

Radiation-induced brain injury involves acute, early delayed and late delayed damage based on the time-course and clinical manifestations. The acute symptoms are mostly transient and reversible, whereas the late delayed radiation-induced changes are progressive and irreversible. Therefore, evaluation of the organ-specific early response to ionizing radiation exposure is necessary for improving treatment strategies and minimizing possible damage at an early stage after radiation exposure.

Reduced distortion in high-index microsphere imaging by partial immersion.

We experimentally demonstrate that pincushion distortion is obvious when using a BaTiO glass (BTG) microsphere fully immersed in ethanol or SU-8 2002 resist to image a Blu-ray disk with sub-diffraction features. The distortion is related to the layer thickness of the immersing medium. For a BTG microsphere partially immersed in the SU-8 resist where the SU-8 thickness is around 4/5 the diameter of the microsphere, its distortion decreases dramatically, but it can still clearly resolve the Blu-ray disk.

Super-resolution imaging properties of cascaded microsphere lenses.

In this paper, the imaging properties of a cascaded microsphere lens are studied. The cascaded microsphere lens consists of two lenses. A hexagonally close-packed 960-nm-diameter array of polystyrene microspheres is used as the first lens.

Influence of the photonic nanojet of microspheres on microsphere imaging.

The imaging properties of BaTiO glass (BTG) microspheres in the diameter range of 5-50 µm which are fully immersed in a polydimethylsiloxane layer are experimentally studied. Our experimental results show that for both Blu-ray disc samples and the single-layer hexagonally close-packed microsphere array samples, with the increase of the diameter of BTG microspheres, the range of focal image positions (RFIP) increases linearly. When the diameter of BTG microspheres increases from 5 to 50 μm, the RFIP changes from 4 to 25 μm.

Mixture Markov regression model with application to mosquito surveillance data analysis.

A mixture Markov regression model is proposed to analyze heterogeneous time series data. Mixture quasi-likelihood is formulated to model time series with mixture components and exogenous variables. The parameters are estimated by quasi-likelihood estimating equations.