Comparative analysis of two novel complete genomes of myxoma virus vaccine strains.

Myxoma virus (MYXV) is a double-stranded DNA-containing virus of the family Poxviridae, genus Leporipoxvirus. MYXV is an important model virus for evolutionary and immunological research and a promising oncolytic. In this study, we sequenced and analyzed two complete genomes of MYXV virus vaccine strains B-82 and Rabbivac-B, which are widely used for vaccine production in Russia.

[Biological characteristics and permissiveness to viruses of diploid kidney cells strain from the bat Nathusius' pipistrelle ( Keyserling & Blasius, 1839; )].

Introduction: Bats are an epidemiologically important natural reservoir of viruses of various taxonomic groups, including causative agents of especially dangerous infections of humans and animals. Considering the relevance of arbovirus infections, it seems advisable to study the spectrum of the sensitivity of cells derived from bats inhabiting and migrating on the territory of the Russian Federation to causative agents of vector-borne diseases of animals.The study aimed to obtain a diploid strain of cells from renal tissue of bats Pipistrellus nathusii and to investigate its biological characteristics, as well as to assess its permissiveness for bluetongue (BTV); Rift Valley fever (RVFV); lumpy skin disease (LSDV); rabbit myxoma (Myxomatosis cuniculi); rabbit, or Shope fibroma (RFV); African horse sickness (AHSV) and African swine fever (ASFV) viruses.

Infection of Bat Epithelial cells Depends on the Invasion Factors InlA and InlB.

is a widespread facultative intracellular pathogen. The range of natural hosts that supporting persistence in the environment has not been fully established yet. In this study, we were interested in the potential of to infect cells of bats, which are being increasingly recognized as a reservoir for microorganisms that are pathogenic to humans and domestic animals.

Identification and characterization of lumpy skin disease virus isolated from cattle in the Republic of North Ossetia-Alania in 2015.

The first notifications of the unknown disease of cattle appeared in September-October 2015 in North Caucasus region of Russia (Republic of North Ossetia-Alania). The clinical signs included watery discharge from eyes, apathy, loss of appetite, salivation, lameness and nodular skin lesions. Capripoxvirus genome was detected by real-time PCR in the tissue samples of sick animals.

[Infectious, immunogenic, and protective characteristics of the Rift Valley fever virus depending on the passage level and storage conditions].

The report discusses the research into the impact of some factors, especially the passage in a suspension of continuous cells BHK-21/13 and storage at different temperatures, upon immunobiological characteristics of the Rift Valley fever (RVF) virus strain 1974-VNIIVViM. The limits for the passage levels and optimal storage conditions providing maximal infectious and immunogenic activity, as well as protection of the attenuated RVF strain 1974- VNIIVViM, were determined. It was found that the RVF virus growth in VHK-21123 cell suspension in the course of 20 consecutive passages and storage at -50 degrees C for 1 to 2 years did not reduce any infectious, immunogenic or protective characteristics of the virus, It was also shown that the RVF virus strain 1974-VNIIVViM could be stored at the following temperature ranges: 1 month at 4 to 6 degrees C, 4 months at -10 to -12 degrees C, 6 months at -20 degrees C, and up to 2 years at -50 degrees C.