Genome Engineering Approaches to Improve Nosokomycin A Production by B38.3.

Here we describe our efforts to improve the levels of phosphoglycolipid antibiotic nosokomycin A production by ATCC14672 via genome engineering approaches. Introduction of two extra copies of leucyl tRNA (UUA) gene and one copy of moenomycin biosynthesis gene cluster led, on average, to threefold increase in nosokomycin A titers (up to 1.5 mg/L).

Analysis of Streptomyces ghanaensis ATCC14672 gene SSFG_07725 for putative γ-butyrolactone synthase.

Low molecular weight signaling compounds (LMWC) are important players in regulating various aspects of Streptomyces biology. Their exact roles in certain strain will ultimately depend on overall configuration of regulatory network and thus cannot be predicted on basis of in silico studies. Here, we explored S.