Characterization of cytoplasmic female sterility in an alloplasmic and monosomic addition line of carrying the cytoplasm and one chromosome of .

Alloplasmic plants exhibit various phenotypic changes such as cytoplasmic male sterility (CMS). We have been attempting to produce an alloplasmic CMS line (2n = 20) carrying cytoplasm (cyt-) for several years, but a single extra chromosome always remained in all lines produced. We confirmed a -specific band in the alloplasmic line carrying cytoplasm by RAPD analysis, indicating that the additional chromosome was derived from .