Correction: Establishment and characterization of noro-VLP measurement by digital ELISA.

Correction for 'Establishment and characterization of noro-VLP measurement by digital ELISA' by Takema Hasegawa , , 2024, , 7089-7094, https://doi.org/10.1039/D4AY01012D.

Establishment and characterization of noro-VLP measurement by digital ELISA.

Highly sensitive viral analytical techniques are essential tools for preventing the spread of infections. In this study, we established a digital enzyme-linked immunosorbent assay (ELISA) system to quantify norovirus proteins with high sensitivity. We used norovirus-like particles (noro-VLPs) as a surrogate for norovirus and constructed two digital ELISA systems using two different antibody pairs.

Recombinant production of antibody antigen-binding fragments with an N-terminal human growth hormone tag in mammalian cells.

Antigen-binding fragments (Fabs) of antibodies are both key biopharmaceuticals and valuable tools for basic life science. To streamline the production of diverse Fabs by capitalizing on standard and highly optimized protein production protocols, we here explore a method to prepare recombinant Fabs as secreted fusion proteins with an N-terminal human growth hormone domain and an octa-histidine tag. These tagged Fabs can be purified with standard immobilized metal chelate affinity chromatography.