Long-Term Exposure to Lambda-Cyhalothrin Reveals Novel Genes Potentially Involved in Insecticide Resistance.

Insecticide resistance in populations hinders vector control programs. Many studies have focused on the classical mechanisms, mutations, and metabolic enzymes to understand the development of insecticide resistance. In this study, we subjected a strain of to selective pressure for 13 consecutive generations to understand the development and extent of insecticide resistance.

Epidemiological features of Leishmania infantum in dogs (Canis lupus familiaris) suggest a latent risk of visceral leishmaniasis in the metropolitan area of Bucaramanga, Santander, Eastern Colombia.

Visceral leishmaniasis (VL) is a disease caused by species of the Leishmania donovani complex that is mainly transmitted through the urban cycle involving dogs as the primary reservoir. In Colombia, the incidence of VL is increasing, along with the spread of potential vectors. This study aims to investigate the eco-epidemiological factors associated with Leishmania spp.

Association of Midgut Bacteria and Their Metabolic Pathways with Zika Infection and Insecticide Resistance in Colombian Populations.

Introduction: is the vector of several arboviruses such as dengue, Zika, and chikungunya. In 2015-16, Zika virus (ZIKV) had an outbreak in South America associated with prenatal microcephaly and Guillain-Barré syndrome. This mosquito's viral transmission is influenced by microbiota abundance and diversity and its interactions with the vector.

Differential Hatching, Development, Oviposition, and Longevity Patterns among Colombian Populations.

Dengue, Zika, and chikungunya are arboviral diseases for which there are no effective therapies or vaccines. The only way to avoid their transmission is by controlling the vector , but insecticide resistance limits this strategy. To generate relevant information for surveillance and control mechanisms, we determined life cycle parameters, including longevity, fecundity, and mortality, of Colombian populations from four different geographical regions: Neiva, Bello, Itagüí, and Riohacha.

Molecular surveillance of resistance to pyrethroids insecticides in Colombian Aedes aegypti populations.

Introduction: In Colombia, organochloride, organophosphate, carbamate, and pyrethroid insecticides are broadly used to control Aedes aegypti populations. However, Colombian mosquito populations have shown variability in their susceptibility profiles to these insecticides, with some expressing high resistance levels.

Materials And Methods: In this study, we analyzed the susceptibility status of ten Colombian field populations of Ae.

The Midgut Microbiota of Colombian Populations with Different Levels of Resistance to the Insecticide Lambda-cyhalothrin.

Insecticide resistance in populations is a problem that hinders vector control and dengue prevention programs. In this study, we determined the susceptibility of populations from six Colombian regions to the pyrethroid lambda-cyhalothrin and evaluated the presence of the V1016I mutation in the sodium channel gene, which has been broadly involved in the resistance to this insecticide. The diversity of the gut microbiota of these mosquito populations was also analyzed.

Susceptibility to Insecticides and Natural Infection in Aedes aegypti: An Initiative to Improve the Mosquito Control Actions in Boyacá, Colombia.

Background: Integrated management strategies for dengue prevention and control have been the main way to decrease the transmission of arboviruses transmitted by in Colombia. However, the increase of chikungunya (CHIKV), Zika, and dengue (DENV) fever cases suggests deficiencies in vector control strategies in some regions from this country.

Objective: This work aimed to establish a baseline susceptibility profile of to insecticides, determine the presence of mutations associated with resistance to pyrethroids, and detect natural arbovirus infection in this vector from Moniquirá - Boyacá, one of the most endemic cities in Colombia.

A Point Mutation V419L in the Sodium Channel Gene from Natural Populations of Aedes aegypti Is Involved in Resistance to λ-Cyhalothrin in Colombia.

Resistance to pyrethroids in mosquitoes is mainly caused by target site insensitivity known as knockdown resistance (). In this work, we examined the point mutations present in portions of domains I, II, III, and IV of the sodium channel gene in mosquitoes from three Colombian municipalities. A partial region coding for the sodium channel gene from resistant mosquitoes was sequenced, and a simple allele-specific PCR-based assay (AS-PCR) was used to analyze mutations at the population level.