Lambs Grazing With Adult Ewes Prefer Forbs With High-Nutrient Content in Native Grasslands Dominated by and .

Grazing livestock in grasslands face the challenge of obtaining sufficient nutrition due to uneven distribution of plant species and fluctuating vegetation productivity and nutrient levels. In northern China, and are the dominant perennial species in native grasslands, but they provide limited nutrition compared to forbs with higher crude protein (CP) content. While dietary ingredients can affect the nutritional intake of grazing livestock, the influence of different grazing strategies on dietary selection remains unclear.

Seed germination and seedling growth response of Leymus chinensis to the allelopathic influence of grassland plants.

Allelopathy has a profound impact on the germination and growth of plants, influencing the establishment of plant populations and shaping community ecological patterns. However, the allelopathic potential of many grassland species remains poorly understood. In this study, we prepared aqueous extracts from 17 herbaceous plants to investigate their allelopathic effects on the seed germination and seedling growth of Leymus chinensis, a dominant grassland species.

Flooding Length Mediates Fencing and Grazing Effects on Soil Respiration in Meadow Steppe.

Grassland management affects soil respiration (Rs, consists of heterotrophic respiration and autotrophic respiration) through soil micro-ecological processes, such as hydrothermal, plant root, organic carbon decomposition and microbial activity. Flooding, an irregular phenomenon in grasslands, may strongly regulate the response of soil respiration and its components to grassland management, but the regulatory mechanism remains unclear. We conducted a 3-year experiment by grassland management (fencing and grazing) and flooding conditions (no flooding (NF), short-term flooding (STF) and long-term flooding (LTF)) to study their effects on Rs and its components in a meadow steppe in the Hui River basin of Hulunbuir.

Nitrogen and phosphorus additions alter foliar nutrient concentrations of dominant grass species and regulate primary productivity in an Inner Mongolian meadow steppe.

Excessive nitrogen (N) inputs shift grassland productivity from nitrogen (N) to phosphorus (P) limitation. However, how plant nutrient concentrations and stoichiometric dynamics at community and species level responding to variable soil N and P availability, and their roles in regulating net primary productivity in meadow steppe remain unclear. To address this issue, we carried out an experiment with fifteen treatments consisting of factorial combinations of N (0, 1.

Alterations of oral microbiota are associated with the development and severity of acute pancreatitis.

Acute pancreatitis (AP) is a common abdomen clinical emergency. Most APs have mild clinical symptoms and a good prognosis. However, about 20% of patients develop severe acute pancreatitis (SAP), increasing morbidity and mortality.

Artificial Grassland Had Higher Water Use Efficiency in Year with Less Precipitation in the Agro-Pastoral Ecotone.

Improving plant water use efficiency is a key strategy for the utilization of regional limited water resources as well as the sustainable development of agriculture industry. To investigate the effects of different land use types on plant water use efficiency and their mechanisms, a randomized block experiment was designed in the agro-pastoral ecotone of northern China during 2020-2021. The differences in dry matter accumulation, evapotranspiration, soil physical and chemical properties, soil water storage and water use efficiency and their relationships among cropland, natural grassland and artificial grassland were studied.

Inhibition of Notch activity promotes pancreatic cytokeratin 5-positive cell differentiation to beta cells and improves glucose homeostasis following acute pancreatitis.

Some individuals develop prediabetes and/or diabetes following acute pancreatitis (AP). AP-induced beta-cell injury and the limited regenerative capacity of beta cells might account for pancreatic endocrine insufficiency. Previously, we found that only a few pancreatic cytokeratin 5 positive (Krt5) cells differentiated into beta cells in the murine AP model, which was insufficient to maintain glucose homeostasis.

Transcriptome analysis revealed gene regulatory network involved in PEG-induced drought stress in Tartary buckwheat ().

Tartary buckwheat is a nutritious pseudo-cereal crop that is resistant to abiotic stresses, such as drought. However, the buckwheat's mechanisms for responding to drought stress remains unknown. We investigated the changes in physiology and gene expression under drought stress, which was simulated by treatment with polyethylene glycol (PEG).

Resveratrol Suppresses Severe Acute Pancreatitis-Induced Microcirculation Disturbance through Targeting SIRT1-FOXO1 Axis.

Background: Resveratrol (RSV), one of the SIRT1 agonists, has the ability of alleviating severe acute pancreatitis (SAP); however, the concrete protective mechanism remains unknown. It is noteworthy that microcirculation disturbance plays a vital role in SAP, and the SIRT1/FOX1 axis can regulate microcirculation. Therefore, this study is aimed at ascertaining what is the underlying mechanism of the protective effect of RSV on SAP, and whether it is associated with alleviating microcirculation disturbance by regulating the SIRT1/FOX1 axis.

Cell differentiation trajectory predicts patient potential immunotherapy response and prognosis in gastric cancer.

The purpose of this study was to investigate the differentiation trajectory of gastric cancer (GC) cells and its clinical relevance and generate a prognostic risk scoring (RS) signature based on GC differentiation-related genes (GDRGs) to predict overall survival (OS). Integrated single-cell RNA sequencing (scRNA-seq) and bulk RNA-seq data from GC samples were used for analysis. The cell differentiation trajectory analysis identified three subsets with distinct differentiation states, of which subsets I/II were involved in metabolic disorders, subset II were also associated with hypoxia tolerance, and subset III were related to immune-related pathways.

Hypoxia-induced lncRNA CASC9 enhances glycolysis and the epithelial-mesenchymal transition of pancreatic cancer by a positive feedback loop with AKT/HIF-1α signaling.

Increasing evidence indicates the dysregulations and pivotal roles of lncRNAs in the development and progression of various cancers, including pancreatic cancer. Enhanced glycolytic flux and epithelial-to-mesenchymal transition (EMT) have been considered as important factors in driving the malignance of pancreatic cancer. Here, we sought to evaluate the biological role and involved mechanism of lncRNA CASC9 (CASC9) in pancreatic cancer.

Co-expression of IL-7 and PH20 promote anti-GPC3 CAR-T tumour suppressor activity in vivo and in vitro.

Background: While CAR-T therapy has successfully treated haematological malignancies, it has proved sub-optimal for solid tumours. The main limitation is the inability of CAR-T cells to infiltrate and then proliferate within tumours.

Method: We co-expressed IL-7 and PH20, a type of hyaluronidase, with CAR targeting GPC3 (G3CAR-7 × 20).

Soil extracellular enzyme activities and the abundance of nitrogen-cycling functional genes responded more to N addition than P addition in an Inner Mongolian meadow steppe.

Nitrogen (N) and phosphorus (P) availability in soils commonly limit belowground biological processes in terrestrial ecosystems. Soil extracellular enzyme activities (EEAs) and microbial functional groups play critical roles in soil biological processes and nutrient cycling, yet their response to nutrient addition are poorly understood. To address this issue, we applied six fertilization treatments composed of combinations of N (0, 1.

Modeling the influence of temperature and water potential on seed germination of L.

L. is a widely distributed perennial herbaceous species in temperate and desert steppes. Relative to other wild species, it produces unique sweet flavors, more biomass in arid and cold environments, and has generated greater interest for crop production.

In situ measurements and meta-analysis reveal that land-use changes combined with low nitrogen application promote methane uptake by temperate grasslands in China.

Methane (CH) oxidation in well-aerated grassland soils is an important sink for atmospheric CH, which can be largely modified by land-use changes. However, the impacts of land-use changes (i.e.

Hypoxia potentiates gemcitabine-induced stemness in pancreatic cancer cells through AKT/Notch1 signaling.

Background: Profound chemoresistance remains an intractable obstacle in pancreatic cancer treatment. Pancreatic cancer stem cells (CSCs) and the ubiquitous hypoxic niche have been proposed to account for drug resistance. However, the mechanism involved requires further exploration.

[Analysis of cervical HPV infection in HIV positive Chinese women].

Objective: To investigate prevalence HPV infections in cervix among HIV-infected Chinese women.

Methods: From September 2009 to May 2011, 293 women with positive HIV underwent cervical cancer screening as study group matched with 200 women with negative HIV as control group. Questionnaires including demographic information and HIV associated information were collected, Pap smear and 23 subtype of HPV were performed in those women.

End-stage liver disease: prevalence, risk factors and clinical characteristics in a cohort of HIV-HCV coinfected Han Chinese.

Introduction: Since the advent of combined antiretroviral therapy (cART), liver-related mortality has become the leading cause of non-AIDS-related deaths in human immunodeficiency virus (HIV) infected patients in Western countries.

Objective: To investigate the incidence, mortality and risk factors of end-stage liver disease (ESLD) in HIV and hepatitis C virus (HCV) coinfected former plasma donors (FPDs) and blood recipients (BRs).

Method: A retrospective study was conducted.

Epidemiologic risk profile of human papillomavirus infections in human immunodeficiency virus-positive Chinese women.

The aim of this study was to evaluate the prevalence and risk factors of human papillomavirus (HPV) infection among human immunodeficiency virus (HIV)-positive women in China. To this end, we enrolled 200 HIV-positive and 182 HIV-negative women in this cross-sectional cohort study. The following sampling methods were used: (i) structured interview, (ii) CD4 cell counts, and (iii) cervical specimens.

[Nevirapine related hepatotoxicity: the prevalence and risk factors in a cohort of ART naive Han Chinese with AIDS].

Objective: To investigate the incidence of hepatotoxicity in acquired immunodeficiency syndrome (AIDS) patients on combined anti-retroviral therapy (cART) containing nevirapine (NVP) and to assess the risk factors and its impact on cART.

Methods: 330 AIDS patients from March 2003 to June 2008 at local county were enrolled and a retrospective study using Kaplan-meier survival and Multivariate logistic regression modeling was conducted.

Results: 267 out of 330 patients received NVP based cART and 63 cases received EFV-based cART.

[Evaluation of fermentation character of alfalfa silage through near infrared reflectance spectroscopy (NIRS)].

It is very important to evaluate the fermentation character of alfalfa silage using near infrared reflectance spectroscopy technology (NIRS) for animal production, including the content of NH3-N, lactic acid, acetic acid and butyric acid in silage. In order to evaluate the feasibility of using NIRS to analyze the formation character of alfalfa silage, the near infrared reflectance spectroscopy models were built for NH3-N, lactic acid, zcetic acid and butyric acid in this experiment. Partial least square regression (PLS), Fourier transform technology and sample preparation with liquid nitrogen technology were used to optimize the model.

[Near infrared determination of dry hay quality in oats].

In the present paper, the analysis of the content of CP, NDF and ADF in the whole dry hay of oats was carried out by using near infrared reflectance spectroscopy (NIRS) technique, and in combination with the partial least square (PLS) regression algorithm the calibration analysis was performed at the same time. The results showed that the calibration models developed by the spectral data pretreatment of the second derivative + Norris smoothing, the multivariate scattering correction + second derivative + Norris smoothing, and the multivariate scattering correction were the best for CP, NDF and ADF with the same spectral regions (9668-4518, 9550-5543, 8943-4042 cm(-1)). All these models yielded coefficients of determination of calibration (r2 cal) for CP and NDF that are both higher than 0.

[Evaluation of fresh sample of alfalfa silage through near infrared reflectance spectroscopy (NIRS)].

It is very important to evaluate the fresh sample of alfalfa silage using near infrared reflectance spectroscopy technology (NIRS) for animal production. The nutrient content of forage means the contents of dry matter (DM), crude protein (CP), neutral detergent fiber (NDF), and acid detergent fiber (ADF) in the forage. Because of the high moisture content, it is difficult to make uniform samples for fresh forage and to get useful information from the spectrum.