Optimized GC-MS method to simultaneously quantify acetylated aldose, ketose, and alditol for plant tissues based on derivatization in a methyl sulfoxide/1-methylimidazole system.

The isomers of monosaccharide always produce multiple chromatographic peaks as volatile derivatives during gas chromatography, which may result in the overlapping of different sugar peaks. Whereas reduction and oximation of sugar carbonyl groups for GC analysis do eliminate many isomer derivatives, the approaches create new problems. One ketose can yield two peaks by oximation, and different aldoses and ketoses can yield the same alditol upon reduction, leading to the inability to detect some important monosaccharides.

Fast and simple droplet sampling of sap from plant tissues and capillary microextraction of soluble saccharides for picogram-scale quantitative determination with GC-MS.

Soluble saccharides are very important metabolites of the life cycle and synthesis of structural polysaccharide components (cellulose, hemicellulose, pectin, etc.) of cell walls. A new method for droplet sampling of saps from tissues of organisms and manipulation routines in capillaries for extraction, derivation, and partitioning were developed for picogram-scale quantitative determination with gas chromatography-mass spectrometry (GC-MS).

Upgrading root physiology for stress tolerance by ectomycorrhizas: insights from metabolite and transcriptional profiling into reprogramming for stress anticipation.

Ectomycorrhizas (EMs) alleviate stress tolerance of host plants, but the underlying molecular mechanisms are unknown. To elucidate the basis of EM-induced physiological changes and their involvement in stress adaptation, we investigated metabolic and transcriptional profiles in EM and non-EM roots of gray poplar (Populus x canescens) in the presence and absence of osmotic stress imposed by excess salinity. Colonization with the ectomycorrhizal fungus Paxillus involutus increased root cell volumes, a response associated with carbohydrate accumulation.