PARP inhibitors sensitize pancreatic ductal adenocarcinoma (PDAC) to radiation by inducing DNA damage and replication stress. These mechanisms also have the potential to enhance radiation-induced type I interferon (T1IFN)-mediated antitumoral immune responses. We hypothesized that the PARP inhibitor olaparib would also potentiate radiation-induced T1IFN to promote antitumor immune responses and sensitization of otherwise resistant PDAC to immunotherapy.
View Article and Find Full Text PDFDiffuse intrinsic pontine glioma (DIPG) is a highly malignant brain tumor that mainly occurs in children with extremely low overall survival. Traditional therapeutic strategies, such as surgical resection and chemotherapy, are not feasible mostly due to the special location and highly diffused features. Radiotherapy turns out to be the standard treatment method but with limited benefits of overall survival.
View Article and Find Full Text PDFPLA2G6-associated neurodegeneration (PLAN, NBIA2) is the second most common type of neurodegeneration with brain iron accumulation (NBIA), caused by recessive mutations of PLA2G6 gene, which encodes Ca-independent phospholipase Aβ (iPLAβ). In most PLAN cases, decreased iPLAβ activity and iron deposition was observed meanwhile, and researchers also identified a PLA2G6 mutation family without iron deposition shown by MRI images. This brought us the question of whether decreased iPLAβ activity was the cause of iron deposition in PLAN.
View Article and Find Full Text PDFPhospholipase A2 group VI (PLA2G6)-associated neurodegeneration (PLAN) includes a series of neurodegenerative diseases that result from the mutations in . PLAN has genetic and clinical heterogeneity, with different mutation sites, mutation types and ethnicities and its clinical phenotype is different. The clinical phenotypes and genotypes of PLAN are closely intertwined and vary widely.
View Article and Find Full Text PDFSmall interfering RNA (siRNA)-induced RNA degradation can specifically inhibit viral infection and has been extensively investigated for its efficacy as an antiviral therapeutic approach. In this study we constructed a lentivirus vector carrying a U6-short hairpin RNA expression cassette to express siRNAs in vero cells. The lentivirus vector also expressed an enhanced green fluorescence protein as a reporter.
View Article and Find Full Text PDFAsian Pac J Cancer Prev
August 2012
Triosephosphate isomerase (TPI), a glycolytic enzyme, functions in catalyzing the interconversion of glyceraldehyde-3-phosphate and dihydroxyacetone phosphate and is generally upregulated in tumours. However, there are data suggesting loss in lymphomas. To determine its effects in chicken embryonal fibroblasts (CEF) a plasmid was constructed to allow transfection.
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