In situ HO generation with gold nanoflowers as the coreactant accelerator for enzyme-free electrochemiluminescent immunosensing.

In traditional electrochemiluminescence (ECL) analysis, gold nanomaterials are commonly used as a tool for signal amplification and linking antibodies due to their good electrical conductivity and biocompatibility. Here, we found that multitipped gold nanoparticles-gold nanoflowers (AuNFs) as coreactant accelerator have good catalytic activity for the reduction of dissolved oxygen (O) to hydrogen peroxide (HO) using tris (hydroxymethyl) aminomethane (Tris) as electron donor. Based on this, a new enzyme-free and label-free ECL immunosensor have been constructed for the detection of α-fetoprotein (AFP).