MDV-encoded protein kinase U3 phosphorylates WTAP to inhibit transcriptomic mA modification and cellular protein translation.

Marek's disease virus (MDV)-encoded U3 is a highly conserved serine/threonine protein kinase in alpha-herpesviruses. In other alpha-herpesviruses, such as pseudorabies virus (PRV), U3 phosphorylates the N6-methyladenosine (mA) methyltransferase Wilms tumor 1-associated protein (WTAP), inhibiting mA modification. However, the role and mechanism of U3-mediated WTAP phosphorylation during MDV infection remain undefined.

An open-pore MFI zeolite nanosheet-modified separator with Li-ion flux regulation for lithium-metal batteries.

Separator modification has become one of the most facile and promising methods to inhibit Li dendrite formation. Herein, an open-pore MFI zeolite nanosheet-modified polyacrylonitrile (open-pore MFI NSs@PAN) separator was prepared the combination of vacuum filtration and the electrospinning technique. The straight channels in the MFI NSs, the fluid channels formed by the stacking of the MFI NSs and the interconnected network channels formed by the interweaving of the PAN nanofibers jointly constructed a micro/nano pore structure, which provides sufficient Li transport channels and enables uniform Li flux.

Fluorometric detection of alpha-fetoprotein based on the use of a novel organic compound with AIE activity and aptamer-modified magnetic microparticles.

Background: Liver cancer is one of the most common cancers in the world, and it seriously threatens human life and health. Alpha-fetoprotein (AFP), as a carcinogenic glycoprotein, is an important serum marker for detecting liver cancer. Therefore, the accurate and sensitive determination of AFP is crucial for the early diagnosis and treatment of liver cancer.

Polyacrylonitrile/UV329/titanium oxide composite nanofibrous membranes with enhanced UV protection and filtration performance.

Ultraviolet (UV) radiation is extremely dangerous to humans and can contribute to immunosuppression, erythema, early ageing and skin cancer. UV protection finishing may greatly influence the handling and permeability of fabrics, while UV-proof fibres can guarantee close contact between UV-resistant agents and fabric without affecting the handling of the fabric. In this study, polyacrylonitrile (PAN)/UV absorber 329 (UV329)/titanium dioxide (TiO) composite nanofibrous membranes with complex, highly efficient UV resistance were fabricated electrospinning.

Fluorine-Free Hydrophobic Modification and Waterproof Breathable Properties of Electrospun Polyacrylonitrile Nanofibrous Membranes.

Waterproof breathable functional membranes have broad application prospects in the field of outdoors textiles. The fluorine-containing microporous membranes of the mainstream functional products easily cause harm to the environment, and thus, the fluorine-free environmental nanofibrous membranes are an important development direction for functional membranes. In this subject, the electrospun polyacrylonitrile nanofibrous membranes were first hydrophobically modified by amino functional modified polysiloxane (AMP), followed by in situ cross-linking modified with 4, 4'-methyl diphenylene diisocyanate (MDI).

Infection phase-dependent dynamics of the viral and host N6-methyladenosine epitranscriptome in the lifecycle of an oncogenic virus in vivo.

Dynamic alteration of the epitranscriptome exerts regulatory effects on the lifecycle of oncogenic viruses in vitro. However, little is known about these effects in vivo because of the general lack of suitable animal infection models of these viruses. Using a model of rapid-onset Marek's disease lymphoma in chickens, we investigated changes in viral and host messenger RNA (mRNA) N6-methyladenosine (m A) modification during Marek's disease virus (MDV) infection in vivo.

A label-free fluorescent aptasensor based on the AIE effect and CoOOH for ultrasensitive determination of carcinoembryonic antigen.

Highly sensitive and specific detection of cancer markers (such as carcinoembryonic antigen) is very important for early diagnosis and treatment of cancer. Herein, we developed a label-free fluorescent aptamer biosensor based on the aggregation-induced emission (AIE) effect and hydroxycobalt oxide (CoOOH) platform, and used it to detect carcinoembryonic antigen (CEA) with high sensitivity and specificity. Fluorescent ionic liquid Compound B can combine with a CEA aptamer (CEA-Apt) through electrostatic attraction and hydrophobic interaction to form an ionic liquid/aptamer (CEA-Apt/B) complex and produce the AIE effect, thereby enhancing the fluorescence intensity of B.

The Human-Specific STING Agonist G10 Activates Type I Interferon and the NLRP3 Inflammasome in Porcine Cells.

Pigs have anatomical and physiological characteristics comparable to those in humans and, therefore, are a favorable model for immune function research. Interferons (IFNs) and inflammasomes have essential roles in the innate immune system. Here, we report that G10, a human-specific agonist of stimulator of interferon genes (STING), activates both type I IFN and the canonical NLRP3 inflammasome in a STING-dependent manner in porcine cells.

Highly sensitive and selective detection of 4-nitroaniline in water by a novel fluorescent sensor based on molecularly imprinted poly(ionic liquid).

A novel molecularly imprinted fluorescent sensor for the determination of 4-nitroaniline (4-NA) was synthesized via free radical polymerization with 3-[(7-methoxy-2-oxo-2H-chromen-4-yl)methyl]-1-vinyl-1H-imidazol-3-ium bromide as the fluorescence functional monomer, 4-NA as the template molecule, ethylene glycol dimethacrylate as the cross-linker, and 2,2'-azo(bisisobutyronitrile) as the initiator. The obtained fluorescent poly(ionic liquid) was characterized through Fourier transform infrared, scanning electron microscopy, Brunauer-Emmett-Teller analysis, and fluorescence spectrophotometry. The fluorescent sensor had high fluorescence intensity, short detection time (0.

2D MOF with electrochemical exfoliated graphene for nonenzymatic glucose sensing: Central metal sites and oxidation potentials.

Two-dimensional metal-organic framework (MOF) has the advantages of high mass transfer speed, tunable porosity, and strong electron transfer capability. The different metal center can give MOF with good electrochemical activity because of the mulriple valence state. Here, a simple and economical method was used to successfully prepare a different metal-coordinated two-dimensional (2D) MOF with electrochemical exfoliated graphene (EG) at room temperature.

Fluorescent aptasensor based on D-AMA/F-CSC for the sensitive and specific recognition of myoglobin.

A novel fluorescent biosensor based on dabcyl [(E)-4-((4-(dimethylamino) phenyl) diazenyl)benzoic acid] -modified anti-Mb aptamer (D-AMA) and 6-FAM(6-carboxyfluorescein) -modified complementary short chain (F-CSC)for the specific and sensitive detection of Mb levels is presented in this study. In PBS buffer solution, D-AMA bound to F-CSC, and then dabcyl quenched the fluorescence of 6-FAM. After adding Mb into the system, D-AMA bound to Mb and separated from F-CSC.

Single-step multivalent capture assay for nucleic acid detection with dual-affinity regulation using mutation inhibition and allosteric activation.

The rational modulation of receptor affinity through distal-site mutation and allosteric control is valuable in biosensor designing to tune the useful dynamic range. Our ability to programmatically engineer dual-affinity regulation into diverse affinities of target binding and activities of hybridization chain reaction, however, remains limited. By programmable engineering of the switching equilibria of the recognition hairpin using distal-site mutation inhibition and allosteric activation, we obtained a set of receptors varying significantly in affinities of target binding and activities of the hybridization chain reaction.

Fluorometric determination of cardiac myoglobin based on energy transfer from a pyrene-labeled aptamer to graphene oxide.

The authors describe a fluorometric assay for cardiac myoglobin (Mb), a marker for myocardial infarction. An Mb-binding aptamer was labeled with pyrene and adsorbed on the surface of graphene oxide (GO) via noncovalent and reversible binding forces. This causes the fluorescence of pyrene (best measured at excitation/emission wavelengths of 275/376 nm) to be quenched.

Simple Synthesis of K₄Nb₆O/C Nanosheets for High-Power Lithium-Ion Batteries with Good Stability.

In this work, a series of two-dimensional (2D) large-size nanosheets were prepared through one-step exfoliation of the huge K₄Nb₆O crystals. The K₄Nb₆O nanosheets with the thickness of about 2 nm was used as the templates of dopamine polymerization and was then carbonized to form C-doped K₄Nb₆O nanosheets. More importantly, the C-doped K₄Nb₆O nanosheets exhibited excellent electrochemical performance with high specific capacity (381 mA h g at 0.

A novel fluorescent aptasensor for the highly sensitive and selective detection of cardiac troponin I based on a graphene oxide platform.

Acute myocardial infarction (AMI) is one of the leading risks to global health. Thus, the rapid, accurate early diagnosis of AMI is highly critical. Human cardiac troponin I (cTnI) has been regarded as a golden biomarker for AMI due to its excellent selectivity.

A new composite of graphene and molecularly imprinted polymer based on ionic liquids as functional monomer and cross-linker for electrochemical sensing 6-benzylaminopurine.

Molecularly imprinted polymers prepared using traditional functional monomers and cross-linkers exhibit slow binding kinetics, low electrocatalytic activity and adsorption capacity. Herein, we report a new composite of ionic liquid-based graphene and molecularly imprinted polymer (IL-GR-MIP) with high electrocatalytic activity and adsorption capacity to construct an effective electrochemical sensor for 6-benzylaminopurine (6-BAP). Our objective was to enhance the efficiency of the sensor by incorporating more IL in the MIP framework.

Electrochemical Molecular Imprinted Sensors Based on Electrospun Nanofiber and Determination of Ascorbic Acid.

In this study, electrochemical molecularly imprinted sensors were fabricated and used for the determination of ascorbic acid (AA). Nanofiber membranes of cellulose acetate (CA)/multi-walled carbon nanotubes (MWCNTs)/polyvinylpyrrolidone (PVP) (CA/MWCNTs/PVP) were prepared by electrospinning technique. After being transferred to a glass carbon electrode (GC), the nanofiber interface was further polymerized with pyrrole through electrochemical cyclic voltammetry (CV) technique.

A novel functional imidazole fluorescent ionic liquid: simple and efficient fluorescent probes for superoxide anion radicals.

Novel imidazole fluorescent ionic liquids with anthracene groups (ImS-FILA) were synthesized for the first time to act as fluorescent probes. They were developed for the determination of superoxide anion radicals (O2 (•-)) in an aqueous system. O2 (•-) was produced by pyrogallol autoxidation.

Synthesis, properties and application research of atrazine Fe₃O₄@SiO₂ magnetic molecularly imprinted polymer.

Introduction: Magnetic Fe3O4 nanoparticles were prepared by coprecipitation and then were coated with SiO2 on the surface.

Materials And Methods: Fe3O4@SiO2 composite microspheres were modified by KH570. Using molecular imprinting technology, atrazine magnetic molecularly imprinted polymer was prepared by using atrazine as template molecule, methacrylic acid as functional monomer and ethylene glycol dimethacrylate as cross-linkers.