Biological evaluation, molecular modeling and dynamics simulation of phenanthrenes isolated from Bletilla striata as butyrylcholinesterase inhibitors.

As part of our continuous studies on natural cholinesterase inhibitors from plant kingdom, the 95% ethanol extract from tubers of Bletilla striata showed promising butyrylcholinesterase (BChE) inhibition (IC = 8.6 μg/mL). The extracts with different polarities (petroleum ether, ethyl acetate, n-butanol, and water) were prepared and evaluated for their inhibition of cholinesterases.

Optimization of an ultrasonic-assisted aqueous two-phase extraction method for four flavonoids from .

An effective approach to extracting four flavonoids (lysioside C, nevadensin-7-sambubioside, ikarisoside B, and nevadensin) from using ultrasonic-assisted aqueous two-phase extraction (UAATPE) technology was studied. Ethanol/KHPO system was selected and the influence of several important parameters, including composition of the aqueous two-phase system (ATPS), extraction temperature and time, particle size, and solvent to material ratio, were investigated by single factor experimentss. Then three key parameters (ethanol concentration, solvent to material ratio and extraction temperature) were further optimized by response surface methodology (RSM).

Strigolactone Analogues Derived from Dihydroflavonoids as Potent Seed Germinators for the Broomrapes.

The broomrapes ( and spp.) and witchweeds ( spp.) are a class of parasitic weeds, which are distributed widely in the tropical, subtropical, and temperate areas of the globe.

Bioactivity-guided identification of flavonoids with cholinesterase and β-amyloid peptide aggregation inhibitory effects from the seeds of Millettia pachycarpa.

Millettia pachycarpa Benth, a widely used anthelminthic drug in folk, is rich in flavonoids with various bioactivities. This study aimed to identify active flavonoids with anti-Alzheimer's disease (AD) effect from its seeds by a bioassay-guided isolation. A novel rotenoid with unusual oxidative ring-opening skeleton (10) and nine known flavonoids (1-9) were obtained, and their structures were elucidated by NMR and HR-ESIMS analysis.

Two new stilbenoids from .

Two new stilbenoids bletilol D () and bletilol E (), together with five known compounds were isolated from . Three of them (, and ) were obtained from this genus for the first time. Their structures were elucidated by spectroscopic methods and comparing with data reported in literatures.