Cloning and Expression Analysis of (Autophagy-Related 8) Gene Family in Solanaceae.

The autophagy-related gene family (Autophagy-related 8) plays an important role in plant growth, development, and stress response. In this study, 15 gene family sequences were amplified from Solanaceae, namely tobacco, tomato, and pepper, using RT-PCR to evaluate their basic properties, protein structure, and function, as well as the role of in autophagy. The physicochemical properties, the predicted secondary and tertiary protein structures, subcellular localisation, gene structures, conserved motifs, and phylogenetic relationships of the genes were analysed using bioinformatic techniques, and their expression patterns under sericin-induced plant disease resistance were investigated by RT-qPCR.