Epigenetic regulation of key gene of PCK1 by enhancer and super-enhancer in the pathogenesis of fatty liver hemorrhagic syndrome.

Objective: Rare study of the non-coding and regulatory regions of the genome limits our ability to decode the mechanisms of fatty liver hemorrhage syndrome (FLHS) in chickens.

Methods: Herein, we constructed the high-fat diet-induced FLHS chicken model to investigate the genome-wide active enhancers and transcriptome by H3K27ac target chromatin immunoprecipitation sequencing (ChIP-seq) and RNA sequencing (RNA-Seq) profiles of normal and FLHS liver tissues. Concurrently, an integrative analysis combining ChIP-seq with RNA-Seq and a comparative analysis with chicken FLHS, rat non-alcoholic fatty liver disease (NAFLD) and human NAFLD at the transcriptome level revealed the enhancer and super enhancer target genes and conservative genes involved in metabolic processes.

Short-chain fatty acids in nonalcoholic fatty liver disease: New prospects for short-chain fatty acids as therapeutic targets.

Nonalcoholic fatty liver disease (NAFLD) is a stress-induced liver injury related to heredity, environmental exposure and the gut microbiome metabolism. Short-chain fatty acids (SCFAs), the metabolites of gut microbiota (GM), participate in the regulation of hepatic steatosis and inflammation through the gut-liver axis, which play an important role in the alleviation of NAFLD. However, little progress has been made in systematically elucidating the mechanism of how SCFAs improve NAFLD, especially the epigenetic mechanisms and the potential therapeutic application as clinical treatment for NAFLD.

Compiling and applying atmospheric resource balance sheet in off-office auditing of leading cadres in China.

Atmospheric resources provide important support for human economic and social systems through their unique ecosystem service functions. Implementing the off-office auditing of atmospheric resources for leading cadres holds great strategic significance for fundamentally solve air pollution problems. Exploring the compilation of an atmospheric resource balance sheet is a necessary pre-step in the implementation of outgoing audits.

Loss of triggers lipid metabolic disorder through H3K27 acetylation-mediated C/EBPβ- activation in non-alcoholic fatty liver disease.

Non-alcoholic fatty liver disease (NAFLD) is associated with mutations in lipopolysaccharide-binding protein ( ), but the underlying epigenetic mechanisms remain understudied. Herein, rats with NAFLD were established and used to conduct integrative targeting-active enhancer histone H3 lysine 27 acetylation (H3K27ac) chromatin immunoprecipitation coupled with high-throughput and transcriptomic sequencing analysis to explore the potential epigenetic pathomechanisms of active enhancers of NAFLD exacerbation upon deficiency. Notably, reduced the inflammatory response but markedly aggravated high-fat diet (HFD)-induced NAFLD in rats, with pronounced alterations in the histone acetylome and regulatory transcriptome.

Epigenetic association study uncovered H3K27 acetylation enhancers and dysregulated genes in high-fat-diet-induced nonalcoholic fatty liver disease in rats.

To evaluate the regulatory landscape underlying the active enhancer marked by H3K27ac in high-fat diet (HFD)-induced nonalcoholic fatty liver disease (NAFLD) in rats. H3K27ac chromatin immunoprecipitation and high-throughput RNA sequencing to construct regulatory profiles and transcriptome of liver from NAFLD rat model induced by HFD. motif analysis for differential H3K27ac peaks.

Is China's Enterprise Employees' Basic Pension Plan Sustainable? Evidence from Two Northeastern Provinces in the Double-Drop Era.

In 2019, the Chinese central government adopted the "double-drop" policy (to reduce both the rate and the base of employers' contributions to employees' basic pension plan) as an attempt to increase overall employment and plan coverage in the national labor market and, in turn, achieve a balance in the plan's annual contributions and benefits at the provincial level. Accordingly, we utilize the cohort element method and actuarial models to explore the policy's effects on the plan's financial sustainability in both the short and long term based on data from Heilongjiang and Liaoning provinces in northeastern China. We further examine the policy's indirect effects (through changes in the compliance rate, enterprises' overall employment rate, coverage rates, or economic growth rates) on the plan's sustainability using the circulation substitution method.

What factors determine the survival of green innovative enterprises in China? -- A method based on fsQCA.

Increasingly serious environmental problems have generated a large number of small and medium-sized green innovative enterprises. Against the background of rapid technological changes and increasingly fierce market competition, survival is the main problem faced by these enterprises. Exploring the mechanisms and core elements that determine the survival of green innovation enterprises is of great practical significance for improving the survival probability of green innovation enterprises and achieving environmental improvement through green innovation.

Ecological security assessment of Chaohu Lake Basin of China in the context of River Chief System reform.

In the face of the increasingly severe water environment, establishing an effective water environment management mechanism is a major concern of governments around the world. The River Chief System attempts to match water environment management to the performance of the main leaders of the local party and government and represents an institutional innovation to solve the problem of river and lake management in China. However, there is still a lack of systematic research on the performance of the River Chief System and how to promote its overall effectiveness.

Functional role of Forskolin and PD166285 in the development of denuded mouse oocytes.

Objective: cAMP and mature promoting factor (MPF) play critical roles during the maturation of mammalian oocytes. The aim of this study was to produce the offspring from denuded oocytes (DOs) in mice by regulating cAMP and MPF.

Methods: In this study, we used DOs at the germinal vesicle (GV) stage in mice and regulated levels of cAMP and MPF in DOs by adding Forskolin and PD166285 during maturation without follicle stimulating hormone and luteinizing hormone, respectively.

Comparative transcriptomic analysis reveals the roles of ROS scavenging genes in response to cadmium in two pak choi cultivars.

To identify key regulatory genes involved in ROS scavenging in response to cadmium (Cd) exposure in pak choi, eight cDNA libraries from Cd-treated and Cd-free roots of two cultivars, Baiyewuyueman (high Cd accumulator) and Kuishan'aijiaoheiye (low Cd accumulator), were firstly performed by RNA-sequencing. Totally 0.443 billion clean reads and 244,190 unigenes were obtained from eight transcriptome.