Fructus Mori polysaccharides modulate the axial distribution of gut microbiota and fecal metabolites to improve symptoms of hyperglycemia in type 2 diabetic mice.

To understand the gut microbiota composition in different intestinal segments is essential to clarify the structure-hypoglycemic relationship of Fructus Mori Polysaccharides (MFP). In this study, the spatial distribution of gut microbiota and fecal metabolites affected by MFP in type 2 diabetes mice was investigated using the 16S rRNA high-throughput sequencing technology and gas chromatography. The results showed that MFP could control body weight and reduce the blood glucose level.

Evaluation of wondfo rapid diagnostic kit (Pf-HRP2/PAN-pLDH) for diagnosis of malaria by using nano-gold immunochromatographic assay.

Prompt and accurate diagnosis is necessary to start adequate treatment for different affecting species including P. falciparum and P. vivax.

Determination of ractopamine in pig hair using liquid chromatography with tandem mass spectrometric detection.

A quantitative analytical procedure for the determination of ractopamine in pig hair has been developed and validated. The hair samples were washed and incubated at 75°C with isoxuprine and hair extraction buffer. The drug present was quantified using mixed solid-phase extraction and liquid chromatography with tandem mass spectrometric detection.

Evaluation of Wondfo influenza A&B fast test based on immunochromatography assay for rapid diagnosis of influenza A H1N1.

Influenza viruses cause significant morbidity and mortality in both children and adults during local outbreaks or epidemics. Therefore, a rapid test for influenza A&B would be useful. This study was conducted to evaluate the clinical performance of the Wondfo influenza A&B test for rapid diagnosis of influenza A H1N1 Infection.

[Interaction between latex microspheres and antibody proteins revealed by fluorescence spectroscopy].

Latex-antibody complexes were prepared by the method of covalent coupling and the properties of the complexes were studied by fluorescence spectrophotometric method for the purpose of revealing the interaction between latex microspheres and antibody proteins. Analysis of intrinsic fluorescence spectra showed that after being coupled with latex microspheres, the emission maximum of antibody protein showed an obvious blue shift, the intensity of emission maximum decreased significantly, the tertiary structure of antibody protein changed to some extent, the interaction between latex microspheres and antibody proteins had a great quenching effect on the intrinsic fluorescence spectra of antibody proteins, the quenching effect was enhanced along with the increasing pH value and latex concentration, and the quenching mechanism was static quenching. Results of exogenous fluorescence spectra showed that the fluorescence intensity of emission maximum was enhanced significantly after being coupled with latex microspheres, the hydrophobicity of antibody protein was decreasing with the increase in the pH values, however, due to the increasing latex concentration, the hydrophobicity antibody protein was increasing.

[FTIR analysis of the impact of covalent coupling on the secondary structure of antibody protein].

The immunolatex was prepared by covalent coupling. FTIR technology combined with substractive spectroscopy, deconvolution, derivation and curve-fitting methods were used to study the structure of the antibody protein on the immunolatex. The result demonstrates that the alpha-helix strcture of antibody increases with the increase in the pH value and the concentration of latex.

Study and evaluation of Wondfo rapid diagnostic kit based on nano-gold immunochromatography assay for diagnosis of Plasmodium falciparum.

Malaria has been recognized as a human disease for thousands of years and remains one of the most common diseases affecting humans worldwide. Therefore, a method for rapidly detecting Plasmodium falciparum is necessary and useful. We have developed Wondfo rapid diagnostic kit based on nano-gold immunochromatography assay for the detection of P.

[Application of ELISA for microcystins detection].

Microcystins (MCs) may be group of closely related toxic cyclic heptapeptides produced by common cyanobacteria (blue-green algae). Their toxicity could be associated with specific inhibition of intracellular protein phosphatases type-1 and type-2A (PP1 and PP2A, respectively). There are some methods for analyzing and detecting MCs in aquatic environment, such as HPLC, LC-MS, PPIA, and ELISA.