Auxin-responsive OsMADS60 negatively mediates rice tillering and grain yield by modulating OsPIN5b expression.

Rice tillering determines grain yield, yet the molecular regulatory network is still limited. In this study, we demonstrated that the transcription factor OsMADS60 promotes the expression of the auxin transporter OsPIN5b to affect auxin distribution and inhibit rice tillering and grain yield. Natural variation was observed in the promoter region of OsMADS60, with its expression level negatively correlated with tiller number and inducible by auxin.

Integrated transcriptomic and metabolomic analyses reveal regulatory networks governing hub metabolic pathways in Fraxinus hupehensis seeds during germination.

Efforts to protect germplasm resources of Fraxinus hupehensis (Oleaceae), an endangered species endemic to Dahong Mountain, Hubei Province, China, are facing difficulties due to the deep dormancy of its seeds. To elucidate the molecular regulatory networks underlying dormancy release, an integrated investigation combining physiological profiling with transcriptomic and metabolomic analyses was performed on seeds of F. hupehensis during six critical germination stages.

Flavonifractor Plautii or Its Metabolite Desaminotyrosine as Prophylactic Agents for Alleviating Myocardial Ischemia/Reperfusion Injury.

Myocardial ischemia/reperfusion (I/R) injury is a major contributor to myocardial damage, leading to adverse cardiac remodeling and dysfunction. Recent studies have highlighted the potential of gut microbiota-derived metabolites in modulating cardiac outcomes. Here, the cardioprotective effects of a commensal bacterium Flavonifractor plautii (F.

Antagonist Targeting the Species-Specific Fatty Acid Dehydrogenase/Isomerase FabX for Anti-H. pylori Infection.

Helicobacter pylori (H. pylori) is a group-1 definite pathogenic carcinogen that infects approximately half of the global population, yet no species-specific chemotherapy has yet been developed. It is previously discovered that H.

Characteristics in patients with unexplained recurrent spontaneous abortion and the impact on immune, coagulation, and inflammatory profiles.

Objective: Unexplained Recurrent Spontaneous Abortion (RSA) impacts the physical and psychological well-being of women of reproductive age. This study aimed to reveal the potential mechanisms behind unexplained RSA from the perspective of glucose and lipid metabolic profiles before conception and their relationship with immune, coagulation, and inflammatory markers.

Methods: A retrospective analysis was conducted on 100 patients with unexplained recurrent spontaneous abortion at our gynecology department from June 2022 to June 2023.

Formation of an outer glassy shell hinders the drying of trehalose droplets.

Background: Airflow drying of mammalian cells has not been successful yet, with one obstacle being the improper drying time of cells-laden trehalose droplets.

Objective: To evaluate the major factors affecting the drying kinetics of aqueous trehalose droplets.

Materials And Methods: A numerical analysis was applied to the evaporation behavior of aqueous trehalose droplets for the preservation of biomaterials.

CT-Based radiomics nomogram of lung and mediastinal features to identify cardiovascular disease in chronic obstructive pulmonary disease: a multicenter study.

Rationale And Objectives: To investigate the performance of two diagnostic models based on CT-derived lung and mediastinum radiomics nomograms for identifying cardiovascular disease (CVD) in Chronic Obstructive Pulmonary Disease (COPD) patients.

Materials And Methods: Hospitalized participants with COPD were retrospectively recruited between September 2015 and April 2023. Clinical data and visual coronary artery calcium score (CACS) were collected.

Network pharmacology and experimental analysis reveal Ethyl caffeate delays osimertinib resistance in lung cancer by suppression of MET.

Background: Fei Yanning Formula (FYN) is extensively applied in clinical lung cancer treatment. However, the specific active constituents and targets of its therapeutic effects remain unclear.

Objective: The study aims to explore the active constituents and mechanism of FYN in delaying osimertinib resistance by network pharmacology analysis and experimental verification.

cfDNA hydroxymethylcytosine profiling for detection metastasis and recurrence of Esophageal Squamous Cell Carcinoma.

Background: A blood-based approach to monitor metastasis and recurrence of esophageal squamous cell carcinoma (ESCC) remains undeveloped. This study aimed to establish a dependable model utilizing cfDNA 5-hydroxymethylcytosines (5hmC) signatures to detect these conditions in ESCC.

Methods: The 5hmC-Seal technique was employed to generate comprehensive 5hmC profiles from the plasma cell-free DNA (cfDNA) of 122 ESCC patients, classified into 72 with metastasis, 50 without metastasis, 30 with recurrence, and 92 without recurrence.

Bionomics and distribution of malaria vectors in Kisumu city, Western Kenya: implications for urban malaria transmission.

Background: Increasing unplanned urbanization in tropical Africa may create new niches for malaria vectors, raising transmission risk, yet control efforts focus on rural ecosystems. Understanding mosquito diversity, ecology and biting behaviour in urban areas is crucial for effective control. This study assessed Anopheles diversity, abundance, behaviour, and Plasmodium infection rates in Kisumu city, Kenya.

Overcoming missed diagnoses of primary central nervous system Lymphoma-The key role of cerebrospinal fluid cytology: a case report.

Background: Central nervous system (CNS) involvement in diffuse large B-cell lymphoma (DLBCL) is relatively rare, occurring at a rate of approximately 5%. Primary CNS lymphoma (CNS-DLBCL), a subtype of DLBCL, is rare clinically but highly malignant and invasive. Its atypical clinical symptoms and imaging features contribute to a high rate of misdiagnosis and a poor prognosis.

Global, regional, and national burden of kidney cancer and attributable risk factors in adults aged 65 years and older from 1990 to 2021 and projections to 2040.

Background: Identifying the past and future burden of kidney cancer (KC) and its temporal trends among older adults (≥ 65 years) at global, regional, and national levels is critical for effective prevention strategies.

Methods: The age-standardized incidence, prevalence, mortality, and disability-adjusted life years (DALYs) were calculated using data from the Global Burden of Disease (GBD) study from 1990 to 2021. These indicators were stratified by sex, age, and socio-demographic index (SDI).

Associations between serum ferritin levels and gestational diabetes mellitus among a non-anemic population.

Background: Studies have shown a strong correlation between excess iron and the development of gestational diabetes mellitus (GDM), though iron is an essential trace element during pregnancy. This study aims to investigate the precise relationship between iron storage levels during late pregnancy and the development of GDM, trying to find out ways to meet pregnant iron storage requirements and reduce GDM risk simultaneously.

Methods: A non-anemic population consisting of 9,512 healthy singleton pregnant women were included in this study.

CBX3 promotes multidrug resistance by suppressing ferroptosis in colorectal carcinoma via the CUL3/NRF2/GPX2 axis.

Chemoresistance poses a significant challenge in colorectal cancer (CRC) treatment. However, the mechanisms underlying chemoresistance remain unclear. CBX3 promoted proliferation and metastasis in CRC.

Association between dietary carotenoid intake and vertebral fracture in people aged 50 years and older: a study based on the National Health and Nutrition Examination Survey.

Unlabelled: This study discussed the association between dietary intake of carotenoids and vertebral fractures by analyzing the target data, concluding a result of negative association and providing valuable information on vertebral fracture.

Objective: This study is to explore the association between dietary intake of carotenoids and vertebral fractures.

Methods: Data of individuals aged ≥ 50 years from the 2013-2014 National Health and Nutrition Examination Survey (NHANES) database were used in this cross-sectional study.

Relationship between serum uric acid levels and metabolism associated fatty liver disease in postmenopausal women based on NHANES 2017-2020.

Studies have shown that postmenopausal women have more metabolic abnormalities than premenopausal women. No consensus exists on how serum uric acid (sUA) affects metabolism-associated fatty liver disease (MAFLD) in postmenopausal women.This prospective observational study examined this link using National Health and Nutrition Examination Survey (NHANES) 2017 to 2020 data.

Chromosome-level genome assembly of a critically endangered species Leuciscus chuanchicus.

Leuciscus chuanchicus, a critically endangered cyprinid endemic in the Yellow River, represents an evolutionary significant lineage within Leuciscinae. However, conservation efforts for this species have been hindered by the lack of genetic and genomic resources. Here we reported a high-quality chromosome-level genome of L.

Heterogeneous effects of genetic variants and traits associated with fasting insulin on cardiometabolic outcomes.

Elevated fasting insulin levels (FI), indicative of altered insulin secretion and sensitivity, may precede type 2 diabetes (T2D) and cardiovascular disease onset. In this study, we group FI-associated genetic variants based on their genetic and phenotypic similarities and identify seven clusters with distinct mechanisms contributing to elevated FI levels. Clusters fall into two types: "non-diabetogenic hyperinsulinemia," where clusters are not associated with increased T2D risk, and "diabetogenic hyperinsulinemia," where T2D associations are driven by body fat distribution, liver function, circulating lipids, or inflammation.

METTL3-mA-mediated TGF-β signaling promotes Fuchs endothelial corneal dystrophy via regulating corneal endothelial-to-mesenchymal transition.

Fuchs endothelial corneal dystrophy (FECD) is the leading cause of vision-threatening corneal endothelial dystrophy without pharmacologic treatments. Corneal endothelial-mesenchymal transition (cEndMT), a specific cellular phenotypic transition, is implicated in the vicious cycle in FECD pathogenesis. Here, we investigated the reversible epigenetic regulation of N-methyladenosine (mA) during cEndMT process and FECD progression.

Reprogramming aerobic metabolism mitigates Streptococcus pyogenes tissue damage in a mouse necrotizing skin infection model.

Disease tolerance is a host response to infection that limits collateral damage to host tissues while having a neutral effect on pathogen fitness. Previously, we found that the pathogenic lactic acid bacterium Streptococcus pyogenes manipulates disease tolerance using its aerobic mixed-acid fermentation pathway via the enzyme pyruvate dehydrogenase, but the microbe-derived molecules that mediate communication with the host's disease tolerance pathways remain elusive. Here we show in a murine model that aerobic mixed-acid fermentation inhibits the accumulation of inflammatory cells including neutrophils and macrophages, reduces the immunosuppressive cytokine interleukin-10, and delays bacterial clearance and wound healing.

Core N-DRC components play a crucial role in embryonic development and postnatal organ development.

Motile cilia and flagella are evolutionarily conserved organelles, and their defects cause primary ciliary dyskinesia (PCD), a disorder characterized by systemic organ dysfunction. The nexin-dynein regulatory complex (N-DRC) is a crucial structural component of motile cilia and flagella, present across various species from Chlamydomonas to humans. Defects in N-DRC components lead to multiple PCD symptoms, including sinusitis and male infertility.

Causal Relationships Between Inflammatory Cytokines and Sepsis: A Mendelian Randomization Study.

Objective: The complex interplay between inflammatory cytokines and sepsis is not well understood. This study employs Mendelian Randomization (MR) to investigate the causal relationships between various inflammatory cytokines and sepsis, aiming to elucidate the underlying mechanisms and potential therapeutic targets.

Methods: This study employed a bidirectional MR approach to investigate the causal effects of inflammatory cytokines on sepsis and vice versa.