Melosuavines A-H, cytotoxic bisindole alkaloid derivatives from Melodinus suaveolens.

Eight new bisindole alkaloids, melosuavines A-C (1-3), having an aspidosperma-scandine linkage, melosuavines D-F (4-6), possessing an aspidosperma-aspidosperma skeleton, and melosuavines G and H (7 and 8) of the aspidosperma-venalatonine type, tenuicausine (9), and melodinine J (10) were isolated from the twigs and leaves of Melodinus suaveolens. The structures of 1-8 were elucidated by extensive spectroscopic methods, and compounds 9 and 10 were identified by comparison with data in the literature. The relative configuration 9 was determined from the ROESY spectrum, and some NMR signals were reassigned.

Serotonin control of thermotaxis memory behavior in nematode Caenorhabditis elegans.

Caenorhabditis elegans is as an ideal model system for the study of mechanisms underlying learning and memory. In the present study, we employed C. elegans assay system of thermotaxis memory to investigate the possible role of serotonin neurotransmitter in memory control.

Pharmacokinetics, tissue distribution and excretion study of dictamnine, a major bioactive component from the root bark of Dictamnus dasycarpus Turcz. (Rutaceae).

Dictamnine is an herbal ingredient isolated from the root bark of Dictamnus dasycarpus Turcz. (Rutaceae). The present study was aimed at the development of an ultra-high performance liquid chromatography-tandem mass spectrometry method to quantify the concentration of dictamnine in rat plasma and tissues for the in vivo pharmacokinetics, tissue distribution and excretion study.

Crucial role of the biological barrier at the primary targeted organs in controlling the translocation and toxicity of multi-walled carbon nanotubes in the nematode Caenorhabditis elegans.

Multi-walled carbon nanotubes (MWCNTs) can be translocated into the targeted organs of organisms. We employed a model organism of the nematode Caenorhabditis elegans to investigate the role of a biological barrier at the primary targeted organs in regulating the translocation and toxicity formation of MWCNTs. A prolonged exposure to MWCNTs at predicted environmental relevant concentrations caused adverse effects associated with both the primary and secondary targeted organs on nematodes.

Biosafety assessment of titanium dioxide nanoparticles in acutely exposed nematode Caenorhabditis elegans with mutations of genes required for oxidative stress or stress response.

We used Caenorhabditis elegans to investigate whether acute exposure to TiO2-NPs at the concentration of 20 μg L(-1) reflecting predicted environmental relevant concentration and 25 mg L(-1) reflecting concentration in food can cause toxicity on nematodes with mutations of susceptible genes. Among examined mutants associated with oxidative stress and stress response, we found that genes of sod-2, sod-3, mtl-2, and hsp-16.48 might be susceptible for TiO2-NPs toxicity.

The in vivo underlying mechanism for recovery response formation in nano-titanium dioxide exposed Caenorhabditis elegans after transfer to the normal condition.

Unlabelled: So far, we still know little about mechanism for recovery response of engineered nanomaterials (ENMs). Here we used Caenorhabditis elegans to investigate recovery responses of titanium dioxide nanoparticles (TiO2-NPs) exposed animals and the underlying mechanism. After acute exposure to TiO2-NPs (100mg/L), endpoints including defecation and permeable state of intestinal barrier of exposed nematodes returned to control levels; however, after prolonged exposure to TiO2-NPs (100μg/L), endpoints of exposed nematodes could not be recovered to control levels under the normal condition.

Pharmacokinetic comparisons of puerarin, daidzin and the glucuronide metabolite of puerarin after administration of total flavonoid from Gegen alone and total flavonoid from Gegen combined with total saponin from Sanqi in rats under different physiological states.

Gegen is one of the most commonly used traditional Chinese medicines for promoting blood circulation and removing blood stasis. Puerarin and daidzin are the main active constituents of Gegen. Puerarin is mainly metabolized in rats by glucuronidation and the major metabolite from rat urine has been identified as puerarin-7-O-glucuronide through semi-preparative HPLC isolation and then spectroscopic analysis.

Simultaneous determination of limonin, dictamnine, obacunone and fraxinellone in rat plasma by a validated UHPLC-MS/MS and its application to a pharmacokinetic study after oral administration of Cortex Dictamni extract.

A rapid and selective ultra high performance liquid chromatography-tandem mass spectrometry method was developed for the simultaneous determination of four major ingredients in Cortex Dictamni extract, including limonin, dictamnine, obacunone and fraxinellone in rats plasma. Nimodipine was used as the internal standard. Following extraction by methyl tert-butyl ether, the analytes were separated on a Thermo Syncronis C18 column by a gradient elution within a runtime of 9min.

[Antibacterial mechanism of sulforaphane on Escherichia coli].

Objective: To investigate the antibacterial mechanism of sulforaphaneon (SFN) on Escherichia coli.

Methods: To determine membrane penetrability, changes of SDS-PAGE protein spectra, soluble protein and alkaline phosphatase and reducing sugar were determined. Cellular nucleic acid synthesis was detected by 4, 6- diamidino-2-phenylindole (DAPI) staining assay.

Simultaneous determination of six isoflavonoids in rat plasma after administration of total flavonoid from Gegen by ultra-HPLC-MS/MS.

A simple, specific, and sensitive ultra-performance liquid chromatography-tandem mass spectrometry method was developed for the simultaneous determination of 3'-hydroxypuerarin, 6''-O-xylosylpuerarin, mirificin, puerarin, 3'-methoxypuerarin and daidzin in rat plasma. After the addition of methanol containing 0.1% formic acid and 10% ascorbic acid, the analytes and rutoside were obtained by protein precipitation, then separated on a Thermo Syncronis C18 column (2.

A fast, sensitive, and high throughput method for the determination of cefuroxime lysine in dog plasma by UPLC-MS/MS.

In order to investigate the preclinical pharmacokinetics of cefuroxime lysine, a fast, sensitive and high throughput UPLC-ESI-MS/MS method has been developed and validated for the quantitative determination of cefuroxime in dog plasma. Cefuroxime and IS phenacetin were extracted from plasma samples by PPT or LLE procedure, and then separated on an ACQUITY UPLC™ BEH C(18) column with an isocratic elution of acetonitrile-0.1% formic acid in 10mM ammonium acetate (40:60, v/v).

Simultaneous determination of ten flavonoids from Viscum coloratum grown on different host species and different sources by LC-MS.

A high-performance liquid chromatographic-mass spectrometric method was developed for the simultaneous determination of 10 flavonoids in Viscum coloratum obtained from different host species and different sources. Viscum coloratum was extracted with 50% methanol. The extracts were separated on a C(18) column with a gradient of 0.

Pharmacokinetics and tissue distribution of docetaxel by liquid chromatography-mass spectrometry: evaluation of folate receptor-targeting amphiphilic copolymer modified nanostructured lipid carrier.

A novel amphiphilic copolymer, folate-poly(PEG-cyanoacrylate-co-cholesteryl cyanoacrylate) (FA-PEG-PCHL) was synthesized to modify docetaxel-loaded nanostructured lipid carrier to lead to a long blood circulating effect and targeting ability for the delivery of antitumor drug in cancer. To investigate the characteristics of modified docetaxel-loaded nanostructured lipid carrier in vivo, a liquid chromatography-mass spectrometry method was developed and validated for the determination of docetaxel in rat plasma and tumor-bearing mouse tissue samples. The biosamples were extracted by liquid-liquid extraction method with ether and separated on a C(18) column (150 mm×4.

Development of the fingerprints for the quality evaluation of Viscum coloratum by high Performance liquid chromatography.

A high-Performance liquid chromatography coupled ultraviolet (HPLC-UV) method was developed for a chemical fingerprint analysis of Eighteen peaks were selected as the common peaks and Homoeriodictyol-7-0-β-D-apiosiyl-(1→2)-β-D-glucoside was used as a reference. The relative areas of common peaks were used for hierarchical clustering analysis and similarity calculation. Thirty-seven samples collected from different sources were classified into five groups.

Pharmacological evaluation of Alstonia scholaris: anti-tussive, anti-asthmatic and expectorant activities.

Ethnopharmacological Relevance: Alstonia scholaris (Apocynaceae) was documented as an effective herb for the treatment of chronic respiratory diseases in "dai" ethnopharmacy historically, and its leaf crude extract, used for releasing tracheitis and cold symptom, was approved to be a commercial formulation by State Food and Drugs Administration of China (SFDA).

Aim Of The Study: The investigation evaluates the anti-tussive and anti-asthmatic activities of the ethanolic extract, fractions and main alkaloids of Alstonia scholaris leaf to provide experimental evidence for its traditional and modern clinical use. For our most interesting, is to reveal the active components for further new drug development.

Pharmacological evaluation of Alstonia scholaris: anti-inflammatory and analgesic effects.

Ethnopharmacological Relevance: Alstonia scholaris (Apocynaceae) has been historically used in "dai" ethnopharmacy to treat chronic respiratory diseases. The leaf extract, developed as a commercially available traditional Chinese medicine, used to release tracheitis and cold symptom, has also been prescribed in hospitals and sold over the counter in drug stores.

Aim Of The Study: The investigation evaluated the anti-inflammatory and analgesic activities of the ethanolic extract, fractions and main alkaloids of Alstonia scholaris leaf to provide experimental evidence for its traditional and modern clinical use.

[Isolation and determination of Neoechinulin A in Cordate Pinellia Tuber].

The Neoechinulin A in Cordate Pinellia Tuber was isolated by column chromatography and identified by mass spectrometry and nuclear magnetic resonance (NMR). A method of reversed-phase high performance liquid chromatography (RP-HPLC) for the determination of the Neoechinulin A in Cordate Pinellia Tuber was developed. The chromatography was performed on a Diamonsil C18 column (250 mm x 4.

[Simultaneous determination of seven effective component in Houpuwenzhong capsules using high performance liquid chromatography].

A high performance liquid chromatographic (HPLC) method was developed for the simultaneous determination of seven effective components in Houpuwenzhong capsules. The separation was performed on a Scienhome C18 column (250 mm x 4.6 mm, 5 microm) with methanol-acetonitrile-0.

[Isolation and determination of ecliptal in Herba Ecliptae by reversed-phase high performance liquid chromatography].

The ecliptal was isolated from Herba Ecliptae by high performance liquid chromatography and identified by mass spectrometry and nuclear magnetic resonance (1H NMR). A method for determination of ecliptal in Herba Ecliptae was developed by using a Kromasil C18 column (200 mm x 4.6 mm, 5 microm) with a mixture of acetonitrile and 0.

[Simultaneous determination of wedelolactone and isodemethylwedelolactone in Herba ecliptae by reversed-phase high performance liquid chromatography].

A reversed-phase high performance liquid chromatographic method (RP-HPLC) was developed for the simultaneous determination of wedelolactone and isodemethylwedelolactone in Herba Ecliptae from different sources. The separation was performed on a Kromasil C18 column (250 mm x 4.6 mm, 5 microm), with methanol-0.

HPLC determination and pharmacokinetic study of homoeriodictyol-7-O-beta-D-glucopyranoside in rat plasma and tissues.

Homoeriodictyol-7-O-beta-D-glucopyranoside (HEDT-Glu) was isolated from Viscum coloratum and identified by MS, 1H- and 13C-NMR. A HPLC method was developed for determination of HEDT-Glu in rat plasma and tissues. All biological samples were pretreated by protein precipitation with acetone.

HPLC determination of safflor yellow A and three active isoflavones from TCM Naodesheng in rat plasma and tissues and its application to pharmacokinetic studies.

A high-performance liquid chromatographic method was developed for the simultaneous determination and pharmacokinetic studies of safflor yellow A, puerarin, 3'-methoxyl-puerarin, and puerarinapioside in the plasma and tissues of rats that had been administered with the traditional Chinese medicine (TCM) preparation Naodesheng via the caudal vein. Samples taken from rats were subjected to protein precipitation with acetone. Separation of these four compounds was accomplished on a Kromisil C18 stationary phase using a mobile phase of acetonitrile-0.

Simultaneous determination of homoeriodictyol-7-O-beta-D-Glccopyranoside and its metabolite homoeriodictyol in rat tissues and urine by liquid chromatography-mass spectrometry.

A liquid chromatographic-mass spectrometric (LC-MS) method was developed and validated for the simultaneous determination of homoeriodictyol-7-O-beta-D-glycoside (HEDT-Glc) and its active metabolite homoeriodictyol (HEDT) in rat tissues and urine. The analytes and internal standard (dihydromyricetin, IS) were detected by using negative atmospheric pressure chemical ionization mass spectrometry in selected ion monitoring (SIM) mode at m/z 464, 301 and 319 for HEDT-Glc, HEDT and IS, respectively. These compounds were eluted on a Luna reverse phase column.

[Isolation and determination of homoeriodictyol-7-O-beta-D-glycoside in Viscum coloratum].

The homoeriodictyol-7-O-beta-D-glycoside was isolated from Viscum coloratum and identified by mass spectrometry and nuclear magnetic resonance (NMR) (1H NMR and 13C NMR). A method for determination of homoeriodictyol-7-O-beta-D-glycoside in Viscum coloratum was developed by using a Kromasil C18 column (200 mm x 4.6 mm i.