Antimicrobial resistance, virulence genes profiles and molecular epidemiology of carbapenem-resistant Klebsiella pneumoniae strains from captive giant pandas (Ailuropoda melanoleuca).

Background: Carbapenem-resistant Klebsiella pneumoniae (CRKP) increases the difficulty of clinical treatment of giant pandas. This study aimed to investigate the antibiotic susceptibility, antibiotic resistance genes (ARGs), mobile genetic elements (MGEs), virulence genes, and molecular epidemiology of CRKP strains isolated from giant pandas. A total of 187 nonduplicated Klebsiella pneumoniae (KP) isolates were collected from fresh feces of captive giant pandas at the Chengdu Research Base of Giant Panda Breeding.

Bright Structural-Phase-Pure CsPbI Core-PbSO Shell Nanoplatelets With Ultra-Narrow Emission Bandwidth of 77 meV at 630 nm.

Achieving a narrow emission bandwidth is long pursued for display applications. Among all primary colors, obtaining pure red emission with high visual perception is the most challenging. In this work, CsPbI halide perovskite nanoplatelets (NPLs) with rigorously controlled 2D  [PbI] octahedron layer number (n) are demonstrated.

A snapshot of climate drivers and temporal variation of abundance from a giant panda living in the wild.

Ticks and tick-borne diseases have negative impacts on the health of wild animals including endangered and vulnerable species. The giant panda (), a vulnerable and iconic flagship species, is threatened by tick infestation as well. Not only can ticks cause anemia and immunosuppression in the giant panda, but also bacterial and viral diseases.

Seroprevalence and risk factors of infection in captive giant panda ().

Introduction: Toxoplasma gondii, a globally zoonotic protozoan parasite, infects most warm-blooded animals including the giant panda, and poses a serious threat to the giant panda conservation. However, the seroprevalence and the risk factors for toxoplasmosis in giant pandas are unknown. Here we aimed to determine the seroprevalence of in the captive population of giant pandas and analyze the factors associated with the increased risk of infection.

Automated machine learning-based model for the prediction of delirium in patients after surgery for degenerative spinal disease.

Objective: This study used machine learning algorithms to identify critical variables and predict postoperative delirium (POD) in patients with degenerative spinal disease.

Methods: We included 663 patients who underwent surgery for degenerative spinal disease and received general anesthesia. The LASSO method was used to screen essential features associated with POD.

Comparison of a commercial ELISA and indirect hemagglutination assay with the modified agglutination test for detection of antibodies in giant panda ().

is a worldwide-distributed zoonotic protozoan parasite which causes toxoplasmosis and has a significant effect on public health. In the giant panda (), toxoplasmosis can cause asymptomatic infections, reproductive disorder and even death, which poses a serious threat to the conservation of this rare protected species. Therefore, serological investigation of is essential to understanding its risk to giant pandas, however, there are no specific testing kits for giant pandas.

Identification of extended-spectrum beta-lactamase (CTX-M)-producing Klebsiella pneumoniae belonging to ST37, ST290, and ST2640 in captive giant pandas.

Background: Extended-spectrum β-lactamases (ESBL)-producing strains of Klebsiella pneumoniae remain a worldwide, critical clinical concern. However, limited information was available concerning ESBL-producing Klebsiella pneumoniae in giant pandas. The objective of this study was to characterize ESBL-producing Klebsiella pneumoniae isolates from captive giant pandas.

High Prevalence of Antimicrobial Resistance and Integron Gene Cassettes in Multi-Drug-Resistant Isolates From Captive Giant Pandas ().

Multi-drug-resistant (MDR ) is increasingly being reported with corresponding increase in morbidity and mortality all over the world. However, limited information is available concerning MDR in giant pandas. The objective of this study was to grasp the drug resistance profile of MDR isolated from giant pandas.

Mortality analysis of captive red panda cubs within Chengdu, China.

Background: The red panda has been classified as an endangered species due to the decreased number in the world and disease is considered as a great threat to the health and survival of the cubs in captivity.

Results: This study analyzed 32 red panda cub mortalities (15 females and 17 males, age less than two months) through gross necropsy, microbiological examination, and histopathological observation at the Chengdu Research Base of Giant Panda Breeding, China, during 2014-2020. The results showed that screenings for canine distemper virus, canine parvovirus, rotavirus and parasite infection were all negative, however bacteria such as Klebsiella pneumoniae, Proteus mirabilis, Escherichia coli, Enterococcus faecalis, Pseudomonas were isolated from the tissue samples of some cubs.

Viral metagenomics unveiled extensive communications of viruses within giant pandas and their associated organisms in the same ecosystem.

Cross-species transmission events were commonplace, with numerous cases of host-switching during the viral evolutionary history, but relatively little evidence for onward transmission in different species living in the same ecosystem. For understanding the communications of viruses in giant pandas (Ailuropoda melanoleuca) and their associated organisms, based on a large size of samples (N = 2305) collected between 2015 and 2020 from giant panda (N = 776) and other four giant panda-associated organisms in the same ecosystem, red pandas (N = 700), stray cats (N = 32), wild rats (N = 42), and mosquitoes (N = 755), viromics was used for the virus identification and subsequent virus traceability. The results showed that a feline panleukopenia virus (FPV) was found in giant pandas with clinical signs of vomiting and mild diarrhea.

Albumin concentration is a candidate indicator for predicting the risk of calf venous thromboembolism in patients undergoing gynecologic surgery.

Objective: To explore the ability of albumin concentration to predict calf venous thromboembolism (cVTE) in gynecologic diseases.

Methods: We analyzed data from 761 patients from the gynecology department. We screened the serum albumin concentration as an important indicator for predicting cVTE through logistic analysis.

First detection and molecular identification of Babesia sp. from the giant panda, Ailuropoda melanoleuca, in China.

Background: Parasitic infections are among the important causes of death of giant pandas (Ailuropoda melanoleuca) that hamper their survival in the wild. There are about 35 species of parasites which have been identified in giant pandas, but no information is currently available regarding the infection of Babesia in giant pandas. Babesia spp.

Isolation and characterization of Uropathogenic Escherichia coli (UPEC) from red panda (Ailurus fulgens).

Background: Disease prevention and control is a significant part in the ex-situ conservation of the endangered red panda (Ailurus fulgens), being bacterial infection is one of the most important health threats to the captive population. To date, studies about the infection caused by Escherichia coli in the red panda are scarce. This study was conducted to determine the cause of death of a captive red panda through clinical symptoms, complete blood count, biochemical analysis, pathological diagnosis and bacterial whole genome sequencing.

Angular color shift of micro-LED displays.

Sidewall emission of a micro-scale light emitting diode (micro-LED) improves the light extraction efficiency, but it causes mismatched angular distributions between AlGaInP-based red micro-LED and InGaN-based blue/green counterparts due to material difference. As a result, color shift of RGB micro-LED displays may become visually noticeable. To address this issue, we first analyze the angular distributions of RGB micro-LEDs and obtain good agreement between simulation and experiment.

Mutation analysis of 13 RM Y-STR loci in Han population from Beijing of China.

Two five-color fluorescence multiplex amplification systems of 13 Y chromosome short tandem repeat (Y-STR) loci (DYF387S1, DYF399S1, DYF403S1a/b, DYF404S1, DYS449, DYS518, DYS526a/b, DYS547, DYS570, DYS576, DYS612, DYS626, and DYS627) have been designed. A study was conducted measuring the mutation rates of the 13 Y-STRs in 501 father-son pairs of Han origin in Beijing, China. Among 7515 allele transmissions in 501 father-son pairs, 75 single-step and 2 two-step mutations were observed.

Diverse and abundant antibiotic resistance genes from mariculture sites of China's coastline.

With the rapid development of mariculture in China, large amounts of antibiotics are being discharged into the aquatic environment. Little information is available regarding antibiotics and corresponding antibiotic resistance genes (ARGs) associated with maricultural environments in China. Sediments from eleven typical mariculture areas along the whole coastline of China were collected, and the sediment in Meijijiao in southern China was used as a non-mariculture control.

[Parecoxib suppresses the increase of neutrophil-to-lymphocyte ratio after the modified radical mastectomy].

To observe the effect of parecoxib on neutrophil-to-lymphocyte ratio (NLR)after the modified radical mastectomy, and to explore its potential mechanisms for inhibition of perioperative inflammation.
 Methods: A total of 40 breast cancer patients undergone the modified radical mastectomy were randomly divided into a parecoxib group (n=20) and a control group (n=20). The parecoxib group received intravenous parecoxib (40 mg, 5 mL) during general anesthesia induction, post-operative day 1 and day 2; the control group received intravenous normal saline (5 mL) at the corresponding time points.

The ventricular late potentials in children with vasodepressor response of vasovagal syncope.

Objectives: To discuss ventricular late potentials (VLPs) in children with vasodepressor response of vasovagal syncope (VVS-V).

Methods: 184 children diagnosed as VVS-V by head-up tilt test (HUTT) were enrolled as VVS-V group, 105 age and gender matched healthy individuals without syncope were used as control group, then signal-averaged electrocardiogram was measured.

Results: HR was decreased [(83.

Dexmedetomidine Inhibits Maturation and Function of Human Cord Blood-Derived Dendritic Cells by Interfering with Synthesis and Secretion of IL-12 and IL-23.

Aims: To investigate the effects and underlying mechanism of dexmedetomidine on the cultured human dendritic cells (DCs).

Methods: Human DCs and cytotoxic T lymphocytes (CTLs) were obtained from human cord blood mononuclear cells by density gradient centrifugation. Cultured DCs were divided into three groups: dexmedetomidine group, dexmedetomidine plus yohimbine (dexmedetomidine inhibitor) group and control group.

Propranolol Attenuates Surgical Stress-Induced Elevation of the Regulatory T Cell Response in Patients Undergoing Radical Mastectomy.

Surgical stress and inflammatory response induce the release of catecholamines and PGs, which may be key factors in facilitating cancer recurrence through immunosuppression. Animal studies have suggested the efficacy of perioperative blockades of catecholamines and PGs in reducing immunosuppression. In this study, to our knowledge, we present the first report of the effects of perioperative propranolol and/or parecoxib on peripheral regulatory T cells (Tregs) in breast cancer patients.

Crystal structure of 1-[(1S,2R)-2-hydroxy-1-methyl-2-phenyl-eth-yl]pyrrolidinium 2-amino-5-chloro-benzoate.

In the cation of the title mol-ecular salt, C13H20NO(+)·C7H5ClNO2 (-), the five-membered ring adopts a twisted conformation about one of the C-N bonds. The exocyclic N-C bond has an equatorial orientation. The dihedral angle between the five-membered ring (all atoms) and the benzene ring is 76.

Interleukin-2 administration after modified radical mastectomy in breast cancer therapy increases peripheral regulatory T cells.

Background: Breast cancer (BC) deaths are a major concern worldwide, and modified radical mastectomy (MRM) still represents a primary therapeutic strategy. Post-surgery administration of interleukin (IL)-2 for BC therapy has been implemented in China recently. Although its impact on regulatory T cells (Tregs) has been documented in some cancer types, such as melanoma, the IL-2-mediated changes in the Treg composition after MRM in BC treatment remain unknown.