A Novel Coumarin Based Fluorescent Probe for Cu²⁺ Detection as well as Applications.

A novel coumarin-based fluorescent probe LY was designed and synthesized in this work. LY could selectively recognize Cu via fluorescence quenching at 522 nm in a DMSO/HO solution. The recognition process experienced minimal interference from other common cations.

Microglia depletion reduces neurodegeneration and remodels extracellular matrix in a mouse Parkinson's disease model triggered by α-synuclein overexpression.

Chronic neuroinflammation with sustained microglial activation occurs in Parkinson's disease (PD), yet the mechanisms and exact contribution of these cells to the neurodegeneration remains poorly understood. In this study, we induced progressive dopaminergic neuron loss in mice via rAAV-hSYN injection to cause the neuronal expression of α-synuclein, which produced neuroinflammation and behavioral alterations. We administered PLX5622, a colony-stimulating factor 1 receptor inhibitor, for 3 weeks prior to rAAV-hSYN injection, maintaining it for 8 weeks to eliminate microglia.

A coumarin based ratiometric fluorescent probe for the detection of Cu and mechanochromism as well as application in living cells and vegetables.

In this paper, a novel coumarin-derived fluorescent probe NY was designed and synthesized. NY displayed a significant ratiometric fluorescence response towards Cu in PBS buffer (10 mM, pH = 7.4), with the emission wavelength blue-shifted from 580 to 495 nm, and a fluorescence change from orange to green was evident under a 365 nm UV light.