Dietary and activity habits associated with hypertension in Kunming school-aged children and adolescents: A multilevel analysis of the study of hypertension risks in children and adolescents.

Objective: Hypertension has become a public health challenge for Yunnan children and adolescents. The study aims is to assess the dietary and activity habits associated with hypertension in Kunming children and adolescents and to develop effective strategies for preventing and controlling, Southwest China.

Methods: Conducted in 2019, the cross-sectional study involved 3,150 students, aged 13.

Secular Trends in Depressive Symptoms in Adolescents in Yunnan, Southwest China From Before COVID-19 to During the COVID-19 Pandemic: Longitudinal, Observational Study.

Background: Yunnan province borders Myanmar, Laos, and Vietnam, giving it one of the longest borders in China. We aimed to determine the trends in prevalence and impact of COVID-19 on depressive symptoms among adolescents (12-18 years) from 2018 to 2022 in Yunnan, southwest China.

Objective: We evaluated the impact of the COVID-19 epidemic on adolescents' mental health, with the aim of reducing the effect of psychological emergency syndrome and promoting healthy, happy adolescent growth.

Community medical service construction: identifying factors that influence medical choice for patients with non-communicable chronic diseases in the Southwest China.

Background: Community medical institutions play a vital role in China's healthcare system. While the number of these institutions has increased in recent years, their construction contents remain insufficient. The potential of community medical institutions in preventing, screening, diagnosing, and treating non-communicable chronic diseases (NCDs) has not been fully utilized.

A study on the influence of community spiritual comfort service on the mental health of older people.

Background: China is experiencing rapid population aging, with the proportion of older adult people aged 60 and above reaching 19. 8% of the total population in 2022. With the growth of age, the physical function of older adults declines and their mental health is getting worse, with the increasing trend of empty nesting and childlessness, older adults lack information and social interaction with others and fall into social isolation, loneliness and some mental health problems, the proportion of older adults with mental health problems gradually rises and the mortality rate increases incrementally, requiring effective ways to intervene in the mental health of older adults and promote healthy aging.

High-risk population and factors of stroke has changed among middle-aged and elderly Chinese-Evidence from 1989 to 2015.

Background: Stroke is an acute cerebrovascular disease with high mortality and disability. This study aimed to investigate the trend of stroke prevalence from 1989 to 2015 in China, explore the transition of high-risk population and high-risk factors, and provide some evidence to develop more targeted stroke intervention strategies.

Material And Methods: We derived the baseline data from China Health and Nutrition Survey (CHNS).

Secular trends of the prevalence of emaciation, overweight, and obesity among school-aged children in Yunnan province, 1985-2019: A serial cross-sectional surveillance study in China.

Objective: To understand the trends of nutrition in children and adolescents, which may further help to prevent and control chronic diseases in younger ages.

Methods: The Chinese National Surveys on Students' Constitution and Health (CNSSCH) in Yunnan is a survey of growth conditions, physical fitness, and health status of students in Yunnan and uses a series of complex multistage stratified sampling of seven prefectures consisting of 16 counties. Sampling schools were held constant over 35 years.

Prevalence trends of hypertension and influence factors among children and adolescents aged 7-17 years in China, 2011-2015: A serial cross-sectional study.

Hypertension has rapidly increased in the last decades throughout the world. It is an emerging disease. However, limited information is available on secular trends and factors of childhood and adolescents' hypertension in China.

Evaluation of stroke health education for primary school students in Dali, China.

Objectives: To provide us with some evidence to develop more targeted stroke intervention strategies, improve the health awareness of stroke among children, and advocate the health promotion campaign of "small hands holding big hands" among parents and children, we have conducted a health education program on stroke among primary school students in Dali.

Methods: This study has applied stratified random cluster sampling in Dali of Yunnan, China. We compared the improvement of students' knowledge of stroke before and after our health education program in primary school students of Dali in October 2020.

Biodegradation of λ-cyhalothrin through cell surface display of bacterial carboxylesterase.

Pyrethroids are the third widespread used insecticides globally which have been extensively applied in agricultural or household environments. Due to continuous applications, pyrethroids have been detected both in living cells and environments. The permanent exposure to pyrethroids have caused substantial health risks and ecosystem concerns.

Analysis of Saccharification Products of High-Concentration Glutinous Rice Fermentation by Q3 and Alcoholic Fermentation of GY-1.

A two-stage process was used to prepare rice alcohol, i.e., saccharification of glutinous rice by Q3, followed by fermentation.

Prevalence trends of hypertension among 9-17 aged children and adolescents in Yunnan, 2017-2019: a serial cross-sectional surveillance survey.

Background: We aim to describe the prevalence and trends of hypertension among 9-17 school-aged students from 2017 to 2019 in Yunnan, China.

Methods: We conducted a cross-sectional study by using data from the Students' Health Surveillance Surveys of 2017, 2018 and 2019 in Yunnan. The Students' Health Surveillance Surveys in Yunnan collected date on physical fitness and health status of students in Yunnan through multistage-stratified sampling in 3 prefectures.

Molecular and Biochemical Characterization of Salt-Tolerant Trehalose-6-Phosphate Hydrolases Identified by Screening and Sequencing Salt-Tolerant Clones From the Metagenomic Library of the Gastrointestinal Tract.

The exploration and utilization of microbial salt-tolerant enzymatic and genetic resources are of great significance in the field of biotechnology and for the research of the adaptation of microorganisms to extreme environments. The presence of new salt-tolerant genes and enzymes in the microbial metagenomic library of the gastrointestinal tract has been confirmed through metagenomic technology. This paper aimed to identify and characterize enzymes that confer salt tolerance in the gastrointestinal tract microbe.

Research Article Product Composition Analysis and Process Research of Oligosaccharides Produced from Enzymatic Hydrolysis of High-Concentration Konjac Flour.

There is a huge variability in reducing sugars, viscosity, and composition of oligosaccharides in the hydrolyzed products of konjac flour with different concentrations. We analyzed the factors affecting reducing sugars, viscosity, and the average degree of polymerization (DP) during the preparation of oligosaccharides from konjac flour hydrolyzed by β-mannanase under the high-concentration solute hydrolysis model. Hydrolysate of konjac flour, using concentrations ranging from 50 to 200 g/L, was directly added into 20 U/mL of β-mannanase solution.

Characterization of EstZY: A new acetylesterase with 7-aminocephalosporanic acid deacetylase activity from Alicyclobacillus tengchongensis.

Deacetyl-7-aminocephalosporanic acid (D-7-ACA) is required for producing of many semisynthetic β-lactam antibiotics; therefore, enzymes capable of converting 7-aminocephalosporanic acid (7-ACA) to D-7-ACA present a valuable resource to the pharmaceutical industry. In the present study, a putative acetylesterase, EstZY, was identified and characterized from a thermophilic bacterium Alicyclobacillus tengchongensis. Sequence alignment showed that EstZY was an acetylesterase which belonged to carbohydrate esterase family 7 (CE7), with substrate preference for short-chain acyl esters p-NPC to p-NPC.

Identification and characterization of an acetyl esterase from Paenibacillus sp. XW-6-66 and its novel function in 7-aminocephalosporanic acid deacetylation.

Objectives: To obtain a new acetyl esterase from Paenibacillus sp. XW-6-66 and apply the enzyme to 7-aminocephalosporanic acid (7-ACA) deacetylation.

Results: The acetyl esterase AesZY was identified from Paenibacillus sp.

Characterization of a novel salt-, xylose- and alkali-tolerant GH43 bifunctional β-xylosidase/α-l-arabinofuranosidase from the gut bacterial genome.

A GH43 bifunctional β-xylosidase encoding gene (XylRBM26) was cloned from Massilia sp. RBM26 and successfully expressed in Escherichia coli. Recombinant XylRBM26 exhibited β-xylosidase and α-l-arabinofuranosidase activities.

Mechanical Measurement System and Precision Analysis for Tactile Property Evaluation of Porous Polymeric Materials.

Tactile properties are one of the most important attributes of porous polymeric materials such as textiles, comprising a subjective evaluation index for textile materials and functional clothing, primarily affecting the sensation of comfort during the wearing of a garment. A new test method was proposed, and a mechanical measurement system was developed to objectively characterize the tactile properties of porous polymeric materials by simulating the dynamic contact processes during human skin contact with the materials and in consideration of different aspects of tactile sensations. The measurement system can measure the bending, compression, friction, and thermal transfer properties in one apparatus, and is capable of associating the objective measurements with the subjective tactile sensations.

Application and Analysis of Mycelia Extending Characteristic in Solid-state Fermentation for Producing Glucoamylase.

Enhanced application of solid-state fermentation (SSF) in industrial production and the influence of SSF of K1 on glucoamylase productivity were analyzed using the flat band method. A growth model was implemented through SSF of K1 in this experiment, and spectrophotometric method was used to determine glucoamylase activity. Results showed that in bran and potato culture medium with 70% moisture in a loose state, μ of mycelium reached to 0.

Enhancing thermal tolerance of Aspergillus niger PhyA phytase directed by structural comparison and computational simulation.

Background: Phytase supplied in feeds for monogastric animals is important for improving nutrient uptake and reducing phosphorous pollution. High-thermostability phytases are particularly desirable due to their ability to withstand transient high temperatures during feed pelleting procedures. A comparison of crystal structures of the widely used industrial Aspergillus niger PhyA phytase (AnP) with its close homolog, the thermostable Aspergillus fumigatus phytase (AfP), suggests 18 residues in three segments associated with thermostability.

Genetic diversity of catechol 1,2-dioxygenase in the fecal microbial metagenome.

Catechol 1,2-dioxygenase is the key enzyme that catalyzes the cleavage of the aromatic ring of catechol. We explored the genetic diversity of catechol 1,2-dioxygenase in the fecal microbial metagenome by PCR with degenerate primers. A total of 35 gene fragments of C12O were retrieved from microbial DNA in the feces of pygmy loris.

[Cloning, heterologous expression and characterization of a thermostable esterase from Bacillus sp. HJ14 for diethyl-phthalate degradation].

Objective: A thermostable esterase EstZ1 from Bacillus sp. HJ14 able to degrade diethyl-phthalate (DEP) was heterologously expressed in Escherichia coli BL21(DE3) and characterized.

Methods: Full-length EstZ1 was obtained based on specific amplification and genome sequencing, and amino acid sequence of EstZ1 was analyzed.

[Gene cloning, heterologous expression and enzyme characterizations of halo-tolerant endoglucanase from Alicyclobacillus D-1].

Objective: An endoglucanase gene (gluE1) was cloned from a thermalacidophilus (Alicyclobacillus tengchongensis CGMCC1504) isolated from a hot spring, and the sequence and biochemical characterization of enzyme were analyzed.

Methods: The full-length gluE1 was obtained based on genome sequencing, analysis of amino acid sequence of GluE1. gluE1 was ligated into pEASY-E2 vector and expressed in Escherichia coli BL21 (DE3) cells.

NaCl-, protease-tolerant and cold-active endoglucanase from Paenibacillus sp. YD236 isolated from the feces of Bos frontalis.

Bos frontalis, which consumes bamboo and weeds, may have evolved unique gastrointestinal microorganisms that digest cellulase. A Paenibacillus sp. YD236 strain was isolated from B.

[Dyslipidemia epidemic characters and relationship in Dai and Jingpo ethnics area of Ruili City].

Objective: To explore the prevalence trends, epidemic characters and relationship of dyslipidemia in Dai and Jingpo ethnics area of Ruili City, Yunnan Province.

Methods: In 2014, the adult residents( aged from 18 to 89) who were in the random cluster sampling family in Ruili and were enrolled for questionnaire survey, physic examination and biochemical tests. Totally 1551 were in this research.