Background: Citrus peels are rich in flavonoids with potential sedative-hypnotic effects; however, these effects have not yet been fully explored. We previously demonstrated that, in mice, standardized lime peel supplement (SLPS) induced non-rapid eye movement sleep and shortened sleep latency through GABA receptors activation.
Purpose And Study Design: This study aimed to evaluate the effects of SLPS in adults experiencing sleep disturbances.
Based on the theory of normative social behavior, this research investigated the moderating role of three forms of sexual stigma - enacted, felt, and internalized - in the relationship between perceived descriptive norms and intentions of gay and bisexual men to engage in three preventive behaviors during the 2022 mpox outbreak in the U.S. Through an online survey conducted among 439 gay and bisexual men in September 2022, we observed that participants' perceived descriptive norms regarding mpox preventive behaviors among gay and bisexual male friends and among gay and bisexual men in general were both positively related to their intentions to adopt the recommended mpox preventive behaviors.
View Article and Find Full Text PDFPolyphenols have been well-established to exert sedative-hypnotic effects in psychopharmacology. Lime (Citrus aurantifolia) peel is rich in biologically active polyphenols; however, the effects of lime peel extract on sleep have not yet been demonstrated. A comparison was conducted in mice, between the sleep-promoting effects of a standardized lime peel supplement (SLPS) and a well-known hypnotic drug, zolpidem, and its hypnotic mechanism was investigated using in vivo and in vitro assays.
View Article and Find Full Text PDFBackground: Diabetes is associated with an increased risk of Parkinson's disease dementia (PDD); however, it is unknown whether this association is dependent on continuous hyperglycemia, hypoglycemic events, or glycemic variability. We aimed to investigate the relationship between visit-to-visit fasting glucose variability and PDD development in patients with Parkinson's disease (PD).
Methods: Using data from the Korean National Health Insurance Service, we examined 9,264 patients aged ≥40 years with Parkinson's disease (PD) who underwent ≥3 health examinations and were followed up until December 2019.
This study examined COVID-19 vaccine videos on TikTok ( = 216 collected in March 2021) during the early days of the vaccine rollout in the U.S., including video source, overall stance toward COVID-19 vaccines, Health Belief Model (HBM)-related content, message features (i.
View Article and Find Full Text PDFBackground: We aimed to investigate the incidence rate of Parkinson's disease dementia (PDD) according to age and disease duration by sex. Furthermore, we explored the effect of each cardiometabolic syndrome and depression on the incidence of PDD.
Methods: Using data from the Korean National Health Insurance Service, 79,622 patients with de novo Parkinson's disease (PD) aged ≥40 years between January 2002 and December 2010 were followed to December 2019.
During times of a pandemic, government emergency response webpages are an important communication channel and if properly managed, will mitigate pandemic impacts. Guided by the Crisis and Emergency Risk Communication (CERC) framework and web interactivity literature, this study examined the information content and interactivity of U.S.
View Article and Find Full Text PDFIn the U.S., e-cigarette companies can apply for permission to use reduced or modified risk messages (MRMs) in their marketing materials.
View Article and Find Full Text PDFObjective: To compare postoperative positional changes in the mandibular proximal segment between the conventional orthognathic surgery (CS) and the surgery-first approach (SF) using intraoral vertical ramus osteotomy (IVRO) in patients with Class III malocclusion.
Methods: Thirty-eight patients with skeletal Class III malocclusion who underwent bimaxillary surgery were divided into two groups according to the use of preoperative orthodontic treatment: CS group (n = 18) and SF group (n = 20). Skeletal changes in both groups were measured using computed tomography before (T0), 2 days after (T1), and 1 year after (T2) the surgery.
Sessile plants are continuously threatened by biotic and abiotic environmental stresses. Since stress responses are in general accompanied by growth retardation, plants in nature should tightly control timing and duration of their stress responses for sustained growth. We previously reported that vesicle-associated membrane protein (VAMP) 721 and 722 are required for growth/development and stress responses in plants.
View Article and Find Full Text PDFExcessive demand for translation and protein folding in the endoplasmic reticulum (ER) can cause ER stress in plants. Here, we show that CALRETICULIN 1 (CRT1) and CRT2 are critical components in the accumulation of VESICLE-ASSOCIATED MEMBRANE PROTEIN 721 (VAMP721) and VAMP722 during ER stress responses. We show that CRT2 interacts with VAMP722 and that CRT1/2 post-translationally maintain elevated VAMP721/722 levels under ER stress.
View Article and Find Full Text PDFVarious forms of protein (proteoforms) are generated by genetic variations, alternative splicing, alternative translation initiation, co- or post-translational modification and proteolysis. Different proteoforms are in part discovered by characterizing their N-terminal sequences. Here, we introduce an N-terminal-peptide-enrichment method, Nrich.
View Article and Find Full Text PDFAlthough the application of patient-specific instruments (PSI) for total knee arthroplasty (TKA) increases the cost of the surgical procedure, PSI may reduce operative time and improve implant alignment, which could reduce the number of revision surgeries. We report our experience with TKA using PSI techniques in 120 patients from March to December 2014. PSI for TKA were created from data provided by computed tomography (CT) scans or magnetic resonance imaging (MRI); which imaging technology is more reliable for the PSI technique remains unclear.
View Article and Find Full Text PDFPurpose: To compare the clinical and radiologic efficacy of adipose-derived stem cells (ADSCs) with fibrin glue and microfracture (MFX) versus MFX alone in patients with symptomatic knee cartilage defects.
Methods: Patients who were aged 18 to 50 years and had a single International Cartilage Repair Society grade III/IV symptomatic cartilage defect (≥3 cm(2)) on the femoral condyle were randomized to receive ADSCs with fibrin glue and MFX treatment (group 1, n = 40) or MFX treatment alone (group 2, n = 40). There was a lack of blinding for patients because of the additional intervention method (liposuction).
Purpose: To compare the relation of extrusion of the graft with the position of the allograft between the parapatellar and transpatellar approaches and to show the primary importance of an anatomically correct position by comparing the chondroprotective effects after lateral meniscal allograft transplantation (MAT) with those of normal healthy knees.
Methods: Geometrical data from patients who underwent magnetic resonance imaging evaluation after lateral MAT were used as baseline input data for 3-dimensional and finite element analysis. The inclusion criteria were patients with symptomatic knees that had undergone meniscectomy who underwent lateral MAT with a minimum follow-up of 2 years.
In Vitro Cell Dev Biol Anim
February 2015
Bone marrow concentration (BMC) is the most recognized procedure to prepare mesenchymal stem cells for cartilage regeneration. However, bone marrow aspiration is highly invasive and results in low stem cell numbers. Recently, adipose tissue-derived stromal vascular fraction (AT-SVF) was studied as an alternate source of stem cells for cartilage regeneration.
View Article and Find Full Text PDFPurpose: This study compared the clinical results and second-look arthroscopic findings of patients undergoing open-wedge high tibial osteotomy (HTO) for varus deformity, with or without mesenchymal stem cell (MSC) therapy.
Methods: This prospective, comparative observational study was designed to evaluate the effectiveness of MSC therapy. The patients were divided into 2 groups: HTO with platelet-rich plasma (PRP) injection only (n = 23) or HTO in conjunction with MSC therapy and PRP injection (n = 21).
Rheumatoid arthritis (RA) and osteoarthritis (OA) are primarily chronic inflammatory diseases. Mesenchymal stem cells (MSCs) have the ability to differentiate into cells of the mesodermal lineage, and to regulate immunomodulatory activity. Specifically, MSCs have been shown to secrete insulin-like growth factor 1 (IGF-1).
View Article and Find Full Text PDFKnee Surg Sports Traumatol Arthrosc
May 2015
Purpose: In the present study, the clinical outcomes and second-look arthroscopic findings of intra-articular injection of stem cells with arthroscopic lavage for treatment of elderly patients with knee osteoarthritis (OA) were evaluated.
Methods: Stem cell injections combined with arthroscopic lavage were administered to 30 elderly patients (≥65 years) with knee OA. Subcutaneous adipose tissue was harvested from both buttocks by liposuction.
Unicomparmental knee arthroplasty (UKA) is a popular alternative to total knee arthroplasty (TKA) and high tibial osteotomy for unicompartmental knee conditions, especially in young patients. However, failure of UKA occurs due to either progressive osteoarthritis (OA) in the other compartment or wear on the polyethylene (PE) insert. This study used finite element (FE) analysis to investigate the effects of PE insert contact pressure and stress in opposite compartments for fixed- and mobile-bearing UKA.
View Article and Find Full Text PDFPurpose: The purpose of this study was to evaluate the clinical and imaging results of patients who received intra-articular injections of autologous mesenchymal stem cells for the treatment of knee osteoarthritis.
Methods: The study group comprised 18 patients (6 men and 12 women), among whom the mean age was 54.6 years (range, 41 to 69 years).
Purpose: The aim of the study was to determine if isolated mesenchymal stem cells (MSCs) derived from the infrapatellar fat pad could effectively improve clinical results when percutaneously injected into arthritic knees.
Level Of Evidence: Therapeutic case-control study; Level III.
Methods: Twenty five stem cell injections combined with arthroscopic debridement were administered to patients with knee OA.
Purpose: To evaluate the incidence of meniscal tears in patients with chronic anterior cruciate ligament (ACL)-deficient knees, and to determine the influence of posterior tibial slope (PTS) on medial meniscal tears in ACL-deficient knees.
Materials And Methods: We reviewed 174 patients (174 knees) with a mean age of 30.7 years who underwent ACL reconstruction for chronic ACL tears.
2-Deoxy-D-glucose (2-DG), a synthetic glucose analog that acts as a glycolytic inhibitor, is currently under clinical evaluation for targeting tumor cells. Tephrosin (TSN), a plant rotenoid, is known as an anticancer agent. In this study, we describe that the addition of TSN to 2-DG enhanced the cytotoxic activity of 2-DG against various types of cancer cells by accelerating ATP depletion and blunting autophagy.
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