Publications by authors named "Yuni Shin"
Article Synopsis
- - Polyhydroxyalkanoate (PHA), particularly P(3HB--3HHx), is a bioplastic whose properties are influenced by the mole fraction of 3-hydroxyhexanoate (3HHx) but faces challenges in mass production due to complex fermentation conditions and strain development.
- - To address production obstacles, researchers blended poly(3-hydroxybutyrate) (P(3HB)) with different 3HHx content films, assessing their molecular weight, thermal, mechanical, and degradability properties.
- - The study found that these blended films maintained similar properties to microbial P(3HB--3HHx) and demonstrated a high degradation rate, suggesting that the simple blending method
Indigo is a unique blue dye that has been used in the textile industry for centuries and is currently mass-produced commercially through chemical synthesis. However, the use of toxic substrates and reducing agents for chemical synthesis is associated with environmental concerns, necessitating the development of eco-friendly alternatives based on microbial production. In this study, a robust industrial strategy for indigo production was developed using Pseudomonas putida KT2440 as the host strain, which is characterized by its excellent ability to degrade aromatic compounds and high resistance to environmental stress.
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- Researchers are tackling the low yield of polyhydroxyalkanoates (PHA) by exploring the use of δ-valerolactone (DVL) and plant oil to boost production without pre-treatment.
- In their study, they found that PhaC enzyme effectively incorporated 5-hydroxyvalerate (5HV) into the PHA, resulting in significant biomass and synthesis during fed-batch fermentation.
- The new PHAs showed improved properties, such as lower melting points and slower degradation rates, demonstrating potential benefits for polymer applications.
Article Synopsis
- Interest in bioplastics is increasing due to environmental pollution, particularly in polyhydroxyalkanoates (PHAs), which are produced by microorganisms.
- The study screened Halomonas species for PHA production using affordable substrates like molasses and soybean flour, leading to the selection of Halomonas cerina YK44 for polyhydroxybutyrate (PHB) production.
- Optimizing the medium showed the best PHB production conditions and revealed that PHB from the new substrates behaved similarly to that made from traditional sugars, although the presence of furfural negatively affected cell growth.
Phospholipid fatty acid (PLFA) analysis is used for characterizing microbial communities based on their lipid profiles. This method avoids biases from PCR or culture, allowing data collection in a natural state. However, PLFA is labor-intensive due to lipid fractionation.
View Article and Find Full Text PDFPolyhydroxyalkanoate (PHA) is an environmental alternative to petroleum-based plastics because of its biodegradability. The polymer properties of PHA have been improved by the incorporation of different monomers. Traditionally, the monomer composition of PHA has been analyzed using gas chromatography (GC) and nuclear magnetic resonance (NMR), providing accurate monomer composition.
View Article and Find Full Text PDFWith an increase in the commercialization of bioplastics, the importance of screening for plastic-degrading strains and microbes has emerged. Conventional methods for screening such strains are time-consuming and labor-intensive. Therefore, we suggest a method for quickly and effectively screening plastic-degrading microbial strains through dual esterase assays for soil and isolated strains, using -nitrophenyl alkanoates as substrates.
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- Polyhydroxyalkanoates (PHA) are gaining attention due to their biodegradability and compatibility, leading to research on bacteria that can produce them.
- A specific strain, BM3-1, was found to produce a significant amount of PHB when using vegetable oil, with soybean oil being the best carbon source.
- BM3-1 also produces a high yield of exopolysaccharides and can be engineered to produce a more complex PHA called P(3HB--3HHx), which has improved physical properties compared to regular PHB.
Polyhydroxyalkanoates (PHAs) are promising alternatives to existing petrochemical-based plastics because of their bio-degradable properties. However, the limited structural diversity of PHAs has hindered their application. In this study, high mole-fractions of Poly (39 mol% 3HB-co-17 mol% 3 HV-co-44 mol% 4 HV) and Poly (25 mol% 3HB-co-75 mol% 5 HV) were produced from 4- hydroxyvaleric acid and 5-hydroxyvaleric acid, using Cupriavidus necator PHB harboring the gene phaC with modified sequences.
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- 5-hydroxyvaleric acid (5-HV) is a key compound for generating valuable chemicals, but its production is limited by the availability of cofactor α-ketoglutaric acid (α-KG).
- Researchers developed a two-cell biotransformation system that uses glutamate oxidase (GOX) to regenerate α-KG, while also incorporating catalase KatE to prevent degradation of α-KG during the process.
- The optimized system produced 1.60 times more 5-HV and achieved significant production of poly(5-hydroxyvaleric acid) (P(5HV)), demonstrating a more effective and economical method for producing these bio-based compounds.
Polybutylene succinate (PBS) stands out as a promising biodegradable polymer, drawing attention for its potential as an eco-friendly alternative to traditional plastics due to its biodegradability and reduced environmental impact. In this study, we aimed to enhance PBS degradation by examining artificial consortia composed of bacterial strains. Specifically, sp.
View Article and Find Full Text PDFL-theanine is an amino acid with a unique flavor and many therapeutic effects. Its enzymatic synthesis has been actively studied and γ-Glutamylmethylamide synthetase (GMAS) is one of the promising enzymes in the biological synthesis of theanine. However, the theanine biosynthetic pathway with GMAS is highly ATP-dependent and the supply of external ATP was needed to achieve high concentration of theanine production.
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- Researchers wanted to help a bacteria called Clostridium acetobutylicum ATCC 824 become better at making hydrogen fuel from plant materials, even when some substances in the plants are harmful to it.
- They found that adding a special protein called phaP made the bacteria more resilient and helped it grow faster, while also increasing its hydrogen production by over two times!
- This study showed that phaP is really helpful for boosting hydrogen production, even in types of bacteria that usually don’t make certain plastics.