Publications by authors named "Yunhai Zhang"

The study presents a method for designing phase masks, specifically the ring-shaped segmentation method, which can be employed in creating the modulation phase for specialized point spread functions (PSFs), such as multi-focus PSFs and those with axial encoding functions. An algorithm for phase inversion optimization is introduced to enhance the optical transfer function efficiency of the designed phase mask, which is based on the Fresnel approximation imaging inverse operation and iterative Fourier transform algorithm. The ring-shaped segmentation phase design approach effectively combines individual phases, resulting in unified PSFs with unique properties.

View Article and Find Full Text PDF
Article Synopsis
  • Horizontal gene transfer (HGT) is a key mechanism influencing evolution and ecology, but its relationship with human activities like nitrogen addition is not fully understood.
  • In a study using both bacterial gene sequencing and metagenomics, researchers discovered that nitrogen additions increased instances of HGT, functional gene diversity, and viral diversity, while simultaneously decreasing bacterial taxonomic diversity.
  • The research also revealed that ceasing nitrogen additions complicated the bacterial cooccurrence network, highlighting the role of specific bacteria in nutrient sharing, which enhances our understanding of community ecology and the impact of anthropogenic factors.
View Article and Find Full Text PDF

We present a design approach for a symmetric power exponential phase, resulting in a phase-modulated point-spread function with two symmetrical mainlobes that can be steered toward the optical axis opposite the x axis. This design offers nanoscale 3D localization capabilities suitable for 3D single-molecule tracking and localization imaging. The axial probing depth of this symmetric power exponential point-spread function can be adjusted as needed by manipulating specific parameters.

View Article and Find Full Text PDF

is known for its tolerance of coarse grains, adaptability, high temperature, humidity, and disease resistance. Primarily, cattle are raised for their meat and milk, and pinpointing genes associated with traits relevant to meat production can enhance their overall productivity. The aim of this study was to identify the genome, analyze the evolution, and explore the function of the gene family in to provide a new molecular target for breeding in meat-quality-trait cattle.

View Article and Find Full Text PDF

METTL3-mediated N6-methyladenosine (m6A) modification is critical for gametogenesis and early embryonic development. However, the function of METTL3-mediated m6A modification in the early development of somatic nuclear transfer embryos (SCNT) remains unclear. Here, we found that METTL3 mRNA and protein levels exhibit dynamic changes during the early development of porcine SCNT embryos.

View Article and Find Full Text PDF

Kif16A, a member of the kinesin-3 family of motor proteins, has been shown to play crucial roles in inducing mitotic arrest, apoptosis, and mitotic cell death. However, its roles during oocyte meiotic maturation have not been fully defined. In this study, we report that Kif16A exhibits unique accumulation on the spindle apparatus and colocalizes with microtubule fibers during mouse oocyte meiotic maturation.

View Article and Find Full Text PDF

According to previous studies, the quality and fertilization rate of fresh sperm from boars of different ages were significantly different. However, the difference of freeze-thaw sperm quality and fertility in boars of different ages is unclear. In this study, boars of a Chinese native breed were assigned into two groups.

View Article and Find Full Text PDF

The suppressor of cytokine signaling 3 (SOCS3) is a key signaling molecule that regulates milk synthesis in dairy livestock. However, the molecular mechanism by which regulates lipid synthesis in goat milk remains unclear. This study aimed to screen for key downstream genes associated with lipid synthesis regulated by in goat mammary epithelial cells (GMECs) using RNA sequencing (RNA-seq).

View Article and Find Full Text PDF

Hydrogen peroxide (HO) synthesis by electrochemical two-electron oxygen reduction has garnered increasing interest as a wide range of potential applications. Gas diffusion electrodes (GDEs) can effectively promote the HO production efficiency by overcoming the oxygen mass transfer limitations but strongly influenced by the electrowetting process along the long-term operation. In this study, the effect of trans-electrode pressure (TEP) of GDE cathode on the electrowetting process was further elucidated.

View Article and Find Full Text PDF

Circular RNA (circRNA) is abundantly expressed in preimplantation embryos and embryonic stem cells in mice and humans. However, its function and mechanism in early development of mammalian embryos remain unclear. Here, we showed that circHIRA mediated miR-196b-5p to regulate porcine early embryonic development.

View Article and Find Full Text PDF
Article Synopsis
  • Testicular health is vital for boar fertility, and m6A methylation plays a key role in the epigenetic regulation of testicular development.
  • A study using MeRIP-seq identified 7,783 m6A peaks in piglet testes and 6,590 in adult testes, with 2,471 peaks common to both.
  • Analysis of differentially methylated and expressed genes highlighted key reproductive genes involved in growth, metabolic regulation, and protein processes, suggesting m6A modification is important in boar testicular development.
View Article and Find Full Text PDF

Trauma and tumor removal usually cause bone defects; in addition, the related postoperative infection also shall be carefully considered clinically. In this study, polylactic acid (PLLA) composite fibers containing Cerium oxide (CeO) are first prepared by electrospinning technology. Then, the PLLA/CeO@PDA/Ag composite materials are successfully prepared by reducing silver ion (Ag) to nano-silver (AgNPs)  coating in situ and binding AgNPs to the materials surface by mussel structure liked polydopamine (PDA).

View Article and Find Full Text PDF

Puberty is considered a prerequisite for affecting reproductive performance and productivity. Little was known about molecular changes in pubertal goat ovaries. Therefore, we measured and performed a correlation analysis of the mRNA and proteins changes in the pre-pubertal and pubertal goat ovaries.

View Article and Find Full Text PDF

Numerous pharmaceuticals and personal care products (PPCPs) co-exist with various types of microplastics (MPs) in the environment, making it extremely hard to experimentally measure all their adsorption interactions. Thus, a precise prediction model is on demand. In this study, we combined the commonly used Dubinin-Astakhov (DA) model and the linear solvation energy relationships (LSERs) model to predict the adsorption capacity (Q) and adsorption affinity (E) of MPs for PPCPs, including the key parameters of MP (specific surface area, oxygen-containing functional groups), and the Kamlet-Taft solvation parameters of organic contaminants.

View Article and Find Full Text PDF

Cellulose nanofiber (CNF) holds great promise in applications such as surface-enhanced Raman scattering (SERS), catalysis, esthesia, and detection. This study aimed to build novel CNF-based SERS substrates through a facile synthetic method. Citrate-reduced gold nanoparticles (AuNPs) were adsorbed on the cationized CNF surface due to electrostatic interactions, and uniform AuNPs@(2,3-epoxypropyl trimethylammonium chloride)EPTMAC@CNF flexible SERS substrates were prepared by a simple vacuum-assisted filtration method.

View Article and Find Full Text PDF
Article Synopsis
  • * A case involving a 60-year-old woman with marginal zone lymphoma highlighted a rare instance of pulmonary and gastric bleeding shortly after receiving iopamidol during a CT scan.
  • * The patient recovered after treatment for an allergic reaction, emphasizing the need to recognize the potential for such complications with iopamidol use, even if they are uncommon.
View Article and Find Full Text PDF

Accumulating evidence has shown that the abnormal toxicity test (ATT) is not suitable as a quality control batch release test for biologics and vaccines. The purpose of the current study was to explore the optimal ATT experimental design for an adenoviral vector-based vaccine product to avoid false positive results following the standard test conditions stipulated in the Pharmacopoeias. ATT were conducted in both mice and guinea pigs based on methods in Pharmacopeias, with modifications to assess effects of dose volume and amount of virus particles (VPs).

View Article and Find Full Text PDF

Prothioconazole (PTC), a novel broad-spectrum triazole fungicide, has attracted widespread concern due to its wide use and toxicological effects on non-target organisms. However, little is known about the impact of PTC on oocyte quality and female fertility, especially on oocyte maturation and fertilization. In the present study, we reported that PTC exposure affects the oocyte developmental competence and oocyte fertilization ability to weaken female fertility.

View Article and Find Full Text PDF

Copper oxides nanoparticles (CuO NPs) are widely used for a variety of industrial and life science applications. In addition to cause neurotoxicity, hepatotoxicity, immunotoxicity, CuO NPs have also been reported to adversely affect the reproductive system in animals; However, little is known about the effects and potential mechanism of CuO NPs exposure on oocyte quality, especially oocyte maturation. In the present study, we reported that CuO NPs exposure impairs the oocyte maturation by disrupting meiotic spindle assembly and chromosome alignment, as well as kinetochore-microtubule attachment.

View Article and Find Full Text PDF

Background: Pregnancy toxemia is a common disease, which occurs in older does that are pregnant with multiple lambs in the third trimester. Most of the sick goats die within a few days, which can seriously impact the economic benefits of goat breeding enterprises. The disease is believed to be caused by malnutrition, stress, and other factors, that lead to the disorder of lipid metabolism, resulting in increased ketone content, ketosis, ketonuria, and neurological symptoms.

View Article and Find Full Text PDF
Article Synopsis
  • A study highlights that a specific pathogen, commonly found in seafood like fish and shrimp, can cause serious bacterial infections leading to gastrointestinal disease and skin infections, with 11 reported cases showing high mortality rates.
  • It documents a case of a 46-year-old man who contracted the infection after contact with contaminated sewage, emphasizing the potential for unconventional transmission routes.
  • The research suggests that thorough patient history is essential for early treatment, particularly for those with weakened immune systems, and stresses the importance of timely surgical interventions like debridement and possible amputation to prevent severe outcomes.
View Article and Find Full Text PDF

Increased atmospheric nitrogen (N) deposition affects biodiversity in terrestrial ecosystems. However, we do not know whether the effects of N on above-ground plant β-diversity are coupled with changes occurring in the soil seed bank. We conducted a long-term N-addition experiment in a typical steppe and found that above-ground β-diversity increased and then decreased with increasing N addition, whereas below-ground β-diversity decreased linearly.

View Article and Find Full Text PDF
Article Synopsis
  • Mortality rates after pancreaticoduodenectomy (PD) have improved, but delayed gastric emptying (DGE) remains a common complication.
  • A study analyzed data from 114 patients who underwent PD to find risk factors for DGE, revealing that 28.9% developed this issue post-surgery.
  • Key risk factors identified include postoperative abdominal complications, preoperative systemic diseases, and early postoperative albumin levels.
View Article and Find Full Text PDF