Elucidation of retention behaviors in reversed-phase liquid chromatography as a function of mobile phase composition.

Various retention models have been widely used for understanding the retention mechanisms of solutes in reversed-phase chromatography systems. The models have been used to interpret the often-observed linear plots of the logarithms of retention factor k versus the solvent modifier concentration C and ln k versus ln⁡C. In this study, the retention behaviors of nine solutes as a function of acetonitrile (ACN) concentration were systematically investigated using a commercially available C column.