Neutral oligosaccharides from ginseng (Panax ginseng) residues vs. neutral ginseng polysaccharides: A comparative study of structure elucidation and biological activity.

This study aimed to compare the structural and biological activities of neutral ginseng residue oligosaccharides (GRO-N) and neutral ginseng polysaccharides (GP-N). Their structures of GRO-N and GP-N were established based on their molecular weight (Mw), monosaccharide composition, Fourier-transform infrared spectroscopy, methylation, and nuclear magnetic resonance analyses. The Mws of GRO-N and GP-N were 1121.

Comparative evaluation of fermented ginseng on alleviating antibiotic-associated diarrhea in mice.

Antibiotic associated diarrhea (AAD) was a common side effect of antibiotics, and fermented ginseng exhibited potential in treating AAD. In this study, the effects of fermented red, white, and black ginseng on AAD were investigated, with a focus on intestinal flora and inflammation. Clindamycin was used to induce AAD in mice, which caused severe diarrhea and weight loss.

Fermented Ginsenosides Alleviate Acute Liver Injury Induced by CCl in Mice by Regulating the AKT/mTOR Signaling Pathway.

This study investigated the alleviating effect of fermented ginsenosides obtained through yeast strain fermentation transformation on acute liver injury (ALI) induced by CCl. Strains were screened for their ability to produce β-glucosidase, the transformation ability of the strain was verified by high-performance liquid chromatography, and the strain F6 was obtained by 26S rRNA sequencing. After fermentation by F6 strain, it was found that the content of ginsenosides Re, Rb, and Rb was significantly decreased ( < 0.

Four-Dimensional Label-Free Quantitative Proteomics of Ginsenoside Rg Ameliorated Scopolamine-Induced Memory Impairment in Mice through the Lysosomal Pathway.

Alzheimer's disease (AD) is a neurodegenerative disease. Ginsenoside Rg has shown potential in treating AD, but the underlying protein regulatory mechanisms associated with ginsenoside Rg treatment for AD remain unclear. This study utilized scopolamine to induce memory impairment in mice, and proteomics methods were employed to investigate the potential molecular mechanism of ginsenoside Rg in treating AD model mice.

Investigation of the structure, rheology and 3D printing characteristics of corn starch regulated by glycyrrhizic acid.

This study aimed to construct a novel corn starch-glycyrrhizic acid (CS-GA) ink and systematically investigate the effects of GA on the water distribution, microstructure, rheology and 3D printing properties of CS hydrogels. The results showed that the CS chains could form strong hydrogen bonds with GA molecules, inhibit the formation of short-range ordered structure of CS and reduce the content of B-type starch. The low-field nuclear magnetic results showed that the introduction of GA could increase bound water content in CS-GA hydrogels.

The correlation between dermoscopy and clinical and pathological tests in the evaluation of skin photoaging.

Background: There are no standards for evaluating skin photoaging. Dermoscopy is a non-invasive detection method that might be useful for evaluating photoaging.

Objective: To assess the correlation between the dermoscopic evaluation of photoaging and clinical and pathological evaluations.

Research on improving the accuracy of laser-induced breakdown spectroscopy analysis by considering plasma attenuation rate characteristics.

Background: Laser-induced breakdown spectroscopy (LIBS) is widely applied in various fields, but accuracy issues limit its further development. Signal uncertainty is the main reason that affects the accuracy of LIBS measurements, but the signal uncertainty caused by different plasmas exhibiting different radiation attenuation rates during the integration time is often neglected. There is a need for a method to correct LIBS signals by quantifying the radiation attenuation rate.

TOF-assisted binocular vision accuracy improvement method for underwater fish size inspection.

To address problems such as the lack of accuracy in acquiring depth maps for dynamic fish 3D measurements by usual binocular vision or a time-of-flight (TOF) depth camera, a TOF-assisted binocular vision depth acquisition algorithm is used to obtain high-quality depth maps. The TOF depth energy function is designed to guide the binocular stereo matching process, which improves the correct matching rate of binocular matching in low-texture regions; the TOF and binocular stereo matching confidence weighting functions are designed to achieve the fusion of the two at pixel level to improve the matching quality of fish in the occluded overlapping regions. The experimental results show that the TOF-assisted binocular vision system improves the accuracy of fish size measurement compared to single binocular vision while reducing the measurement error when the fish body has a significant inclination along the depth axis.

Ginsenoside Rk1 inhibits HeLa cell proliferation through an endoplasmic reticulum signaling pathway.

Background: Changes to work-life balance has increased the incidence of cervical cancer among younger people. A minor ginseng saponin known as ginsenoside Rk1 can inhibit the growth and survival of human cancer cells; however, whether ginsenoside Rk1 inhibits HeLa cell proliferation is unknown.

Methods And Results: Ginsenoside Rk1 blocked HeLa cells in the G0/G1 phase in a dose-dependent manner and inhibited cell division and proliferation.

Effects of different transcranial magnetic stimulations on neuropathic pain after spinal cord injury.

Introduction: Repetitive transcranial magnetic stimulation (rTMS) is an effective non-invasive cortical stimulation technique in the treatment of neuropathic pain. As a new rTMS technique, intermittent theta burst stimulation (iTBS) is also effective at relieving pain. We aimed to establish the pain-relieving effectiveness of different modalities on neuropathic pain.

Critical genes in human photoaged skin identified using weighted gene co-expression network analysis.

Photoaging is unique to the skin and is accompanied by an increased risk of tumors. To explore the transcriptomic regulatory mechanism of skin photoaging, the epidermis, and dermis of 16 healthy donors (eight exposed and eight non-exposed) were surgically excised and detected using total RNA-Seq. Weighted gene co-expression network analysis (WGCNA) identified the most relevant modules with exposure.

Norcantharidin Nanostructured Lipid Carrier (NCTD-NLC) Suppresses the Viability of Human Hepatocellular Carcinoma HepG2 Cells and Accelerates the Apoptosis.

Malignant tumors have become the main cause of harm to human life and health. Development for new antitumor drugs and the exploration to drug carriers are becoming the concerned focus. In this study, we exploited our experiments to explore the effect of NCTD-NLC on liver cancer cells: the HepG2 cells cultured in vitro were given with NCTD-NLC administration; then, the estimation on cellular proliferation and apoptosis was accomplished through MTT and flow cytometry.

Comparative proteomics profiling revealed the involvement of GRB2-ROCK2 axis in Lyme neuroborreliosis caused by Borrelia Burgdorferi.

The zoonotic Lyme neuroborreliosis (LNB) disease is caused by Borrelia burgdorferi, with wide distribution, rapid dissemination and high disability rate. However, the molecular mechanism underlying B. burgdorferi mediated neuroborreliosis remains largely unknown.

An Analysis of the Correlation Between Clinical Indexes and Pathological Classifications in 202 Patients with Lupus Nephritis.

Objective: To investigate the correlation between clinical indexes and pathological classifications in 202 patients with lupus nephritis (LN).

Methods: A total of 202 LN cases were retrospectively analyzed. All these patients met the four diagnostic criteria for systemic lupus erythematosus (SLE) of the American College of Rheumatology revised in 1997.

Integrative Transcriptome and Proteome Analyses Provide New Insights Into the Interaction Between Live Borrelia burgdorferi and Frontal Cortex Explants of the Rhesus Brain.

Borrelia burgdorferi (Bb), which is neurotropic, can attack the central nervous system (CNS), leading to the development of various neurologic symptoms. The pathogenesis of Lyme neuroborreliosis (LNB) remains poorly understood. Presently, there is a lack of knowledge of the changes in mRNA and proteins in the CNS following early disseminated Lyme disease.

Salp15, a Multifunctional Protein From Tick Saliva With Potential Pharmaceutical Effects.

ticks are the main vectors for a number of zoonotic diseases, including Lyme disease. Ticks secrete saliva directly into a mammalian host while feeding on the host's blood. This action serves to modulate host immunity and coagulation, thus allowing ticks to attach and feed upon their host.

Immunogenicity and Safety of the M72/AS01 Candidate Vaccine Against Tuberculosis: A Meta-Analysis.

Currently, there is no tuberculosis (TB) vaccine recommended for use in latent TB infections and healthy adults. M72/AS01 is a new peptide vaccine currently under development, which may improve protection against TB disease. This vaccine has been investigated in several phase I/II clinical trials.

Rhesus Brain Transcriptomic Landscape in an Model of the Interaction of Live With Frontal Cortex Tissue Explants.

Lyme neuroborreliosis (LNB) is the most dangerous manifestation of Lyme disease caused by the spirochete which can reach the central nervous system most commonly presenting with lymphocytic meningitis; however, the molecular basis for neuroborreliosis is still poorly understood. We incubated explants from the frontal cortex of three rhesus brains with medium alone or medium with added live for 6, 12, and 24 h and isolated RNA from each group was used for RNA sequencing with further bioinformatic analysis. Transcriptomic differences between the model of live with rhesus frontal cortex tissue explants and the controls during the progression of the infection were identified.

Enzymatic transformation of ginsenosides Re, Rg1, and Rf to ginsenosides Rg2 and aglycon PPT by using β-glucosidase from Thermotoga neapolitana.

Objectives: To enzymatically transform protopanaxatriol by using β-glucosidase from Thermotoga neapolitana (T. neapolitana) DSM 4359.

Results: Recombinant β-glucosidase was purified, which molecular weight was about 79.

basic membrane protein A stimulates murine macrophage to secrete specific chemokines.

In this study, we investigated the mechanisms that lead to the production of proinflammatory mediators by the murine macrophage cell line, RAW264.7, when these cells are exposed in vitro to recombinant Borrelia burgdorferi basic membrane protein A (rBmpA). Using antibody protein microarray technology with high-throughput detection ability for detecting 25 chemokines in culture supernatant the RAW264.