Degradation pathways of the pesticide pyridaben in tea caused by ultraviolet photocatalytic degradation were identified using SERS and GC-MS. Pyridaben in tea decreased from 4.50 mg/kg to 3.
View Article and Find Full Text PDFThiram is a readily synthesized, cost-effective antimicrobial agent widely used to control diseases in fruits and vegetables. Given the potential health hazards associated with thiram residues and advancements in detection methods, it is crucial to develop a rapid and sensitive technique for detecting these residues on fruit surfaces. Here, we prepared the Ag@filter paper (Ag@FP) surface-enhanced Raman scattering (SERS) substrate in a controlled manner and innovatively developed a capillarity-assisted SERS (CA-SERS) detection method.
View Article and Find Full Text PDFA highly sensitive immunoadsorption assay without traditional horseradish peroxidase for signal amplification was developed, utilizing a three-way DNA junction amplifier instead. The formation of a three-way DNA junction and release of trigger DNA with recycling was accomplished by toehold-mediated strand displacement. The fluorescent dye -methyl mesoporphyrin IX, with highly structure-specific binding affinity towards G-quadruplexes, was employed for label-free and simple signal output.
View Article and Find Full Text PDFBiosens Bioelectron
March 2025
A rapid, low-cost and reliable interfacial plasmonic nanoarray is presented as surface-enhanced Raman scattering (SERS) sensing platform for preliminary quantification and identification of flavonoids. Here, CTAB-modified Au colloidal nanoparticles self-assemble at the cyclohexane/acetone-water interface to form a uniform interfacial plasmonic nanoarray. The target hydrophobic analytes including organic dye methyl red and water-insoluble flavonoids, are effectively captured at the air-water interface and enter the "hot spots" between nanoparticles during the evaporation of the oil phase, which contributes to sensitive and reproducible SERS signals.
View Article and Find Full Text PDFObjective: This study aimed to investigate the effects of lipopolysaccharide (LPS)-pretreated primary periodontal ligament stem cell (PDLSC)-derived extracellular vesicles (EVs) (L-PDLSC-EVs) on periodontitis.
Materials And Methods: PDLSCs were obtained from mouse periodontal ligaments via enzymatic digestion. An in vitro inflammatory microenvironment for PDLSCs was established using LPS, and L-PDLSC-EVs were isolated through ultracentrifugation and identified.
In this study, the laccase-mimicking enzyme MnO/Cu-BDC-His was synthesized by a facile procedure, and was applied in tetracycline antibiotics (TCs) identification and dye degradation. The MnO/Cu-BDC-His nanozymes effectively recognized phenolic hydroxyl groups in TCs and catalyzed the generation of colored oxidation products with different characteristic absorbance peaks at 350 nm, 525 nm and 600 nm. Different TCs mixtures produced different absorbance intensities at the above wavelengths and exhibited cross-color responses.
View Article and Find Full Text PDFUnlabelled: Porcine epidemic diarrhea (PED) has caused serious economic losses to the swine livestock industry. Due to the rapid variation in the PEDV) genome, especially the spike (S) protein, the cross-protection ability of antibodies between different vaccine strains is weakened. Hence, the rapid development of safe, broad-spectrum and highly effective attenuated PEDV vaccine still needs further research.
View Article and Find Full Text PDFEcotoxicol Environ Saf
October 2024
Microplastics (MPs) are synthetic solid particles or polymer matrices that range in size from 1 μm to 5 mm. MPs are widely present in the global biosphere, leading to increasing concerns about their impact on human health. In this study, micro-Raman spectroscopy was used to evaluate the presence and characteristic of MPs in adenomyosis, ovarian ectopic cysts, and uterine tube tissue samples from 60 females.
View Article and Find Full Text PDFElectrochromic smart windows (ESWs) are an effective energy-saving technology for near-zero energy buildings. They consume electric energy unidirectionally during a round-trip coloring-bleaching process, with the energy involved in the bleaching process being wasted. It is highly desirable to reuse this wasted electric energy directly and/or transfer it into other energy storage equipment, further enhancing the overall efficiency of electric energy usage.
View Article and Find Full Text PDFYam is a dual-purpose crop used in both medicine and food that is commonly used as a dietary supplement in food processing. Since yam proteins are often lost during the production of yam starch, elucidating the functionally active value of yam proteins is an important guideline for fully utilizing yam in industrial production processes. This study aimed to explore the potential protective effect of yam protein (YP) on cyclophosphamide (CTX)-induced immunosuppression in mice.
View Article and Find Full Text PDFAdv Food Nutr Res
August 2024
Current analytical methods utilized for food safety inspection requires improvement in terms of their cost-efficiency, speed of detection, and ease of use. Sensor array technology has emerged as a food safety assessment method that applies multiple cross-reactive sensors to identify specific targets via pattern recognition. When the sensor arrays are fabricated with nanomaterials, the binding affinity of analytes to the sensors and the response of sensor arrays can be remarkably enhanced, thereby making the detection process more rapid, sensitive, and accurate.
View Article and Find Full Text PDFMicroplastics (MPs) pollution has received widespread attention in recent years as the use of plastics continues to increase. However, currently no studies have reported the finding of MPs in human uterine fibroids (UFs) and myometrium tissues. In this study, UFs tissues (n = 48) and myometrium tissues (n = 40) from 48 patients and myometrium tissues (n = 8) from healthy population were collected.
View Article and Find Full Text PDFPlastic pollution at the nanoscale continues to pose adverse effects on environmental sustainability and human health. However, the detection of nanoplastics (NPLs) remains challenging due to limitations in methodology and instrumentation. Herein, a "green approach" for surface-enhanced Raman spectroscopy (SERS) was exploited to detect polystyrene nanospheres (PSNSs) in water, employing untreated filter paper and a simple syringe-filtration set-up.
View Article and Find Full Text PDFBackground: Mycotoxin contamination of food has been gaining increasing attention. Hidden mycotoxins that interact with biological macromolecules in food could make the detection of mycotoxins less accurate, potentially leading to the underestimation of the total exposure risk. Interactions of the mycotoxins alternariol (AOH) and alternariol monomethyl ether (AME) with high-molecular glutenin were explored in this study.
View Article and Find Full Text PDFEnviron Geochem Health
July 2024
Microplastics (MPs) can enter the reproductive system and can be potentially harmful to human reproductive health. In this study, 13 types of microplastics (MPs) were identified in patient blood, cancer samples, and paracarcinoma samples using Raman spectroscopy, with polyethylene, polypropylene and polyethylene-co-polypropylene being the most abundant polymer types. Futher, cotton was also found in our study.
View Article and Find Full Text PDFA novel laccase mimic enzyme Cu-Mn with excellent photothermal properties was firstly prepared via a combination of hydrothermal and in situ synthesis. Cu-Mn nanozymes could catalyze the typical laccase substrate 2,4-dichlorophenol (2,4-DP) to generate the red quinone imine. Further, loading the MnO nanosheets with photothermal properties, Cu-Mn nanozymes possessed not only excellent laccase catalytic activity, but also high photothermal conversion efficiency.
View Article and Find Full Text PDFBiomimetic cytochrome P450 for chemical activation of environmental carcinogens is an efficient model for evaluating their mutagenicity and ultimately acquiring the metabolites that cannot be easily accessed by conventional routes of organic synthesis. Different kinds of mutagen derived from polyaromatic hydrocarbons (PAHs) by metalloporphyrin/oxidant model systems have been reported, but the underlying molecular mechanisms are poorly understood. Herein, we have for the first time demonstrated an effective surface-enhanced Raman scattering (SERS) protocol to study the dynamics and biomimetic metabolic behaviors of pyrene (Pyr) in the presence of various oxygen donors.
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