Evaluation and validation of reference genes in for real-time quantitative PCR.

For all organs at all stages, , and can be selected as reference genes. For organs of the vegetative stage, and can be chosen for analysis of normalized gene expression. For the bud stage, and can be used for analysis of gene expression.

[Cloning and expression analysis of 8 bHLH transcription factors in Panax quinquefolius].

A total of 8 bHLH transcription factors were cloned from Panax quinquefolius and the response of them to methyl jasmonate(MeJA) was studied.To be specific, based on the preliminary transcriptome screening, 8 bHLH transcription factors were cloned with seedlings which had been cultured for 3 weeks.The content of ginsenosides Rg_1, Re, and Rb_1, and total saponins in the adventitious roots of P.

Selection and validation of reference genes for quantitative real-time PCR in .

Selection of reference genes (RGs) is important for the accurate analysis of real-time quantitative PCR (RT-qPCR) results. This study screened RGs of  for more accurate quantification of target genes. The two most stable RGs for all tissues were and , those for vegetative organs were and , while those for reproductive organs as well as organs in the full flowering stage were and .

Pelagic larval dispersal habits influence the population genetic structure of clam Gomphina aequilatera in China.

Pelagic larval dispersal habits influence the population genetic structure of marine mollusk organisms via gene flow. The genetic information of the clam Gomphina aequilatera (short larval stage, 10 days) which is ecologically and economically important in the China coast is unknown. To determine the influence of planktonic larval duration on the genetic structure of G.

Molecular cloning and spatiotemporal expression of an APETALA1/FRUITFULL-like MADS-box gene from the orchid (Cymbidium faberi).

In order to identify genes involved in floral transition and development of the orchid species, a full-length APETALA1/FRUITFULL-like (AP1/FUL-like) MADS box cDNA was cloned from Cymbidium faberi (C. faberi) sepals and designated as C. faberi APETALA1-like (CfAP11], JQ031272.