A tracer model nowcast/forecast study of the Tampa Bay, Piney Point effluent plume: Rapid response to an environmental hazard.

An emergency discharge of nutrient-rich effluent from the defunct Piney Point fertilizer stack into Tampa Bay at Port Manatee occurred from 30 March-8 April 2021. This resulted in a pollutant plume that evolved over time and space across the entire bay, including its environmentally sensitive marine preserves, and out onto the adjacent continental shelf. As a rapid response to environmental concerns, the plume evolution was simulated using the high resolution, unstructured grid, Tampa Bay Coastal Ocean Model (TBCOM) nowcast/forecast system, with an embedded tracer module that included realistic point discharge rates.

Expeditious assembly of biuret-guanidine derivatives via the catalyst-free transformation.

We disclose a mild and practical catalyst-free transformation for the expeditious construction of biuret-guanidine derivatives using aromatic isocyanates. This synthetic transformation is featured with mild reaction conditions and high efficiency.

Zeylenone synergizes with cisplatin in osteosarcoma by enhancing DNA damage, apoptosis, and necrosis via the Hsp90/AKT/GSK3β and Fanconi anaemia pathway.

A safer and more effective combination strategy designed to enhance the efficacy and minimize the toxicity of cisplatin in osteosarcoma (OS) is urgently needed. Zeylenone (zey), a cyclohexene oxide compound, exerted an obvious inhibitory effect on several cancer cell lines and exhibited little cytotoxicity towards normal cells, enabling zey to play a unique role in combination therapy. Thus, the study aimed to determine whether the combination of zey and cisplatin produces synergistic antitumour effects on OS and to further explore molecular mechanisms.

Fabrication of chitin nanofiber-PDMS composite aerogels from Pickering emulsion templates with potential application in hydrophobic organic contaminant removal.

Natural polymers have aroused increasing attention in water treatment but their application in removing hydrophobic organic contaminants (HOCs) was limited due to their hydrophilicity. Herein, hydrophobic aerogels were successfully fabricated from Pickering emulsions stabilized by chitin nanofibers (ChNF) with polydimethylsiloxane (PDMS) as dispersed phase and glutaraldehyde as a crosslinking agent, and their performance in HOCs removal were evaluated. The Pickering emulsions with PDMS ratios of 2.

The pathogenicity of or aerobactin-positive in infected mice.

Objectives: To investigate the pathogenicity of (KPN) possessing or the aerobactin gene in infected mice.

Methods: BALB/c mice were divided into four groups (n = 10 per group) and infected with: string test-positive and -positive or aerobactin-positive KPN (group 1), string test-negative but -positive KPN (group 2), string test-negative but aerobactin-positive KPN (group 3), or string test- and /aerobactin-negative KPN (group 4). Mouse survival time was compared among groups, and the infection of livers, spleens, lungs, and kidneys and KPN growth were assessed in infected mice.

Bacteremia and other body site infection caused by hypervirulent and classic Klebsiella pneumoniae.

Objectives: To investigate bacteremia and other body site infection caused by hypervirulent Klebsiella pneumoniae (hvKP), a recently recognized pathogen of invasive infection, and classic Klebsiella pneumoniae (cKP), a very common organism associated with many kinds of nosocomial infection.

Methods: Clinical information obtained from patients with both bacteremia and other body site infections caused by hvKP and/or cKP was retrospectively reviewed. Homo-hvKP (or homo-cKP) was defined as homologous hvKP (or cKP) strains from different body sites in each individual patient according to string test, virulence gene amplification and PFGE pattern.

Clinical and Molecular Analysis of Klebsiella pneumoniae Causing Liver Abscess in China.

Objective: Hypervirulent Klebsiella pneumoniae (hvKP) has been characterized as the major pathogen associated with the development of liver abscesses. Although the prevalence of K. pneumoniae in liver abscesses in Chinese patients is on the rise, methods for the early laboratory identification and recognition of hvKP are lacking, and hvKP detection and surveillance are thus limited.

[The clinical and bacterial features of Klebsiella pnuemoniae liver abscess].

Objective: To summarize the clinical and bacterial features of Klebsiella pnuemoniae liver abscess (KPLA) in order to provide the basis for the diagnosis and treatment of KPLA.

Methods: Retrospective study was conducted. One hundred and fifty-two medical records, from 3 teaching hospitals in Beijing, between January 2010 and December 2014, were collected.

Global epigenomic analysis indicates that epialleles contribute to Allele-specific expression via Allele-specific histone modifications in hybrid rice.

Background: For heterozygous genes, alleles on the chromatin from two different parents exhibit histone modification variations known as allele-specific histone modifications (ASHMs). The regulation of allele-specific gene expression (ASE) by ASHMs has been reported in animals. However, to date, the regulation of ASE by ASHM genes remains poorly understood in higher plants.

The OsSec18 complex interacts with P0(P1-P2)2 to regulate vacuolar morphology in rice endosperm cell.

Background: Sec18p/N-ethylmaleimide-sensitive factor (NSF) is a conserved eukaryotic ATPase, which primarily functions in vesicle membrane fusion from yeast to human. However, the function of the OsSec18 gene, a homologue of NSF in rice, remains unknown.

Results: In the present study, we investigated the function of OsSec18 in rice and found that OsSec18 complements the temperature-sensitive phenotype and interferes with vacuolar morphogenesis in yeast.

A SNP in OsMCA1 responding for a plant architecture defect by deactivation of bioactive GA in rice.

Plant architecture directly affects biomass in higher plants, especially grain yields in agricultural crops. In this study, we characterized a recessive mutant, plant architecture determinant (pad), derived from the Oryza sativa ssp. indica cultivar MH86.

Global RNA sequencing reveals that genotype-dependent allele-specific expression contributes to differential expression in rice F1 hybrids.

Background: Extensive studies on heterosis in plants using transcriptome analysis have identified differentially expressed genes (DEGs) in F1 hybrids. However, it is not clear why yield in heterozygotes is superior to that of the homozygous parents or how DEGs are produced. Global allele-specific expression analysis in hybrid rice has the potential to answer these questions.

Therapeutic effect of recombinant plasmid-encoded human interleukin-12 in tumor-bearing mice.

The aim of this study was to investigate the effects of gene therapy using a recombinant plasmid encoding human interleukin-12 (rIL-12, pcDNA6-p70) on transplanted tumors in mice. Tumor-bearing mice were transplanted with sarcoma‑180 (S-180) cells and randomly divided into three groups of 10 mice with each group receiving a separate treatment. Following this, pcDNA6-p70 (dissolved in purified water; 100 µg/mouse), cyclophosphamide (dissolved in 0.

Large-scale production of functional human serum albumin from transgenic rice seeds.

Human serum albumin (HSA) is widely used in clinical and cell culture applications. Conventional production of HSA from human blood is limited by the availability of blood donation and the high risk of viral transmission from donors. Here, we report the production of Oryza sativa recombinant HSA (OsrHSA) from transgenic rice seeds.

Proteomic analysis of rice endosperm cells in response to expression of hGM-CSF.

The accumulation of significant levels of transgenic products in plant cells is required not only for crop improvement, but also for molecular pharming. However, knowledge about the fate of transgenic products and endogenous proteins in grain cells is lacking. Here, we utilized a quantitative mass spectrometry-based proteomic approach for comparative analysis of expression profiles of transgenic rice endosperm cells in response to expression of a recombinant pharmaceutical protein, human granulocyte-macrophage colony stimulation factor (hGM-CSF).