Native N-glycome profiling of single cells and ng-level blood isolates using label-free capillary electrophoresis-mass spectrometry.

The development of reliable single-cell dispensers and substantial sensitivity improvement in mass spectrometry made proteomic profiling of individual cells achievable. Yet, there are no established methods for single-cell glycome analysis due to the inability to amplify glycans and sample losses associated with sample processing and glycan labeling. In this work, we present an integrated platform coupling online in-capillary sample processing with high-sensitivity label-free capillary electrophoresis-mass spectrometry for N-glycan profiling of single mammalian cells.

Social media insights into spatio-temporal emotional responses to COVID-19 crisis.

The Coronavirus pandemic has presented multifaceted challenges in urban emotional well-being and mental health management. Our study presents a spatio-temporal sentiment mining (STSM) framework to address these challenges, focusing on the space-time geography and environmental psychology. This framework analyzes the distribution and trends of 6 categories of public sentiments in Shanghai during the COVID-19 crisis, considering the potential urban spatial influencing factors.

In-capillary sample processing coupled to label-free capillary electrophoresis-mass spectrometry to decipher the native N-glycome of single mammalian cells and ng-level blood isolates.

The development of reliable single-cell dispensers and substantial sensitivity improvement in mass spectrometry made proteomic profiling of individual cells achievable. Yet, there are no established methods for single-cell glycome analysis due to the inability to amplify glycans and sample losses associated with sample processing and glycan labeling. In this work, we developed an integrated platform coupling online in-capillary sample processing with high-sensitivity label-free capillary electrophoresis-mass spectrometry for N-glycan profiling of single mammalian cells.

Trends and hotspots for European Journal of Medicinal Chemistry: A bibliometric study.

European Journal of Medicinal Chemistry (EJMC) has been around for a long time and has gained broad interest from the various individuals working in the field. However, there is no bibliometric analysis on the publications of EJMC to thoroughly assess the scientific output and current status systematically. Therefore, the study was conducted to analyze the various publications of EJMC from 1987 to 2022 to improve their quality.

On-capillary Cell Lysis Enables Top-down Proteomic Analysis of Single Mammalian Cells by CE-MS/MS.

Proteomic analysis of limited samples and single cells requires specialized methods that prioritize high sensitivity and minimize sample loss. Consequently, sample preparation is one of the most important steps in limited sample analysis workflows to prevent sample loss. In this work, we have eliminated sample handling and transfer steps by processing intact cells directly in the separation capillary, online with capillary electrophoresis coupled to tandem mass spectrometry (CE-MS/MS) for top-down proteomic (TDP) analysis of low numbers of mammalian cancer cells (<10) and single cells.

Signature Ions Triggered Electron-Transfer/Higher-Energy Collisional Dissociation (EThcD) for Specific and Confident Glycation Site Mapping in Therapeutic Proteins.

Higher-energy collisional dissociation (HCD) is a well-established fragmentation technique in liquid chromatography tandem mass spectrometry (LC-MS/MS) and is used to study protein post translational modifications (PTMs) during peptide mapping. However, labile PTMs like glycosylation, glycation, sulfonylation, or phosphorylation tend to fragment earlier than peptide backbones under HCD. This leads to complicated MS/MS spectra, compromising data quality and downstream data interpretation.