In vitro model of bacterial biofilm formation on polyvinyl chloride biomaterial.

The aim of the study was to establish an in vitro model of Staphylococcus epidermidis biofilms on polyvinyl chloride (PVC) material, and to investigate bacterial biofilm formation and its structure using the combined approach of confocal laser scanning microscope (CLSM) and scanning electron microscope (SEM). Staphylococcus epidermidis bacteria (stain RP62A) were incubated with PVC pieces in Tris buffered saline to form biofilms. Biofilm formation was examined at 6, 12, 18, 24, 30, and 48 h.

[Effects of enalapril on NF-kappaB expression in mucus hypersecretion rats induced by acrolein exposure].

Objective: To investigate the effects of enalapril on nuclear factor-kappa B (NF-kappaB) expression in a rat model of mucus hypersecretion induced by acrolein.

Methods: The rat model of mucus hypersecretion was established by the inhalation of acrolein. Then the mucus hypersecretion rats were treated by enalapril.

[Serum and lung tissue selenium measurements in subjects with lung cancer from Xuanwei, China].

Background And Objective: Xuanwei is an area of the highest incidence and mortality with lung cancer in China. The aim of this study is to determine serum selenium concentrations in lung cancer patients from Xuanwei as well as selenium levels of cancerous tissues, cancer-adjacent pulmonary tissues, and normal pulmonary lung tissues from lung cancer patients, and the relationship between selenium and the high incidence of lung cancer in Xuanwei.

Methods: One hundred and twenty female adults from Xuanwei were enrolled in the study (60 lung cancer patients and 60 with non-tumor and non-respiratory diseases, respectively) and blood samples were collected.

[Therapeutic efficacy of Traditional Vein Chemotherapy and Bronchial Arterial Infusion Combining with CIKs on III Stage Non-small Cell Lung Cancer.].

Background: The therapeutic efficacy of late lung-cancer was very poor, and cytokine-induced killer cells (CIK) were paid more attention to treat non-small cell lung cancer (NSCLC). The aim of this study is to get insight into the role of bronchial arterial infusion bronchial arterial infusion (BAI) plus CIK about NSCLC by comparing therapeutic efficacy among BAI, traditional vein chemotherapy and BAI plus CIK, for late NSCLC.

Methods: A total of 120 patients were enrolled in this study, dividing randomly into three groups: bronchial arterial infusion (BAI), traditional vein chemotherapy and BAI plus CIK.

[Effect of Suture Lines on Lung Adenocarcinoma Cell in vitro.].

Background: The interaction of cell and medical biomaterial is one of the significant factors to affect clinical application of medical biomaterial. This research is to investigate three of suture lines how to affect the proliferation and cell cycle of Lung Adenocarcinoma cell A549.

Methods: Three of suture lines are respectively cultivated with Lung Adenocarcinoma cell A549, after of 72 hours, to detect absorptance of each groups by MTT method in order to reflect the proliferation of Lung Adenocarcinoma cell A549 and to detect percentage of G1 period cell and S period cell of each of groups by flow cytometry.

[Effect of brominated furanones on biofilm formation of Escherichia coli on polyvinyl chloride material].

Objective: To study the influence of brominated furanones on the biofilm formation of Escherichia coli on the polyvinyl chloride (PVC) material, and to provide new ideas for the research of surface modification of materials and clinical treatment of biomaterial centered infection.

Methods: Three brominated furanones with representative chemical structure were chosen and coated on the surface modification of PVC materials, respectively [furanone 1: 3, 4-dibromo-5-hydroxy-furanone; furanone 2: 4-bromo-5-(4-methoxyphenyl)-3-(methylamino)-furanone; furanone 3: 3, 4-dibromo-5, 5-bis (4-methylphenyl)-2 (5H)-furanone]. All the modificated PVC materials and Escherichia coli were co-cultivated.

[Expression of PAH-DNA adducts in lung tissues of Xuanwei female lung cancer patients].

Background And Objective: The coal-fired pollution in Xuanwei area has been considered to be local main reason for high incidence of female lung cancer. The aim of this study is to explore the expression of PAH-DNA adducts in lung tissues of Xuanwei female lung cancer patients and to explore the relationship between the large number of coal-fired pollution PAHs materials and the high incidence of Xuanwei female lung cancer.

Methods: We totally collected each 20 cases of Xuanwei female lung cancer patients, Xuanwei male lung cancer patients, Non-Xuanwei female lung cancer patients and collect each 10 cases of Xuanwei, Non-Xuanwei female patients with benign lung lesions.

[Research on fast track surgery application in lung cancer surgery].

Background And Objective: Fast track surgery (FTS) is a systematical method to accelerate the recovery of surgical patients by reducing the physical and mental trauma stress of them. The research is to investigate the feasibility of FTS application in lung cancer surgery.

Methods: A total of 80 cases of lung cancer patients with single leaf lobotomy resection were randomized into two groups.

[Distribution of 33 elements in lung cancer tissues, paracancerous lung tissues and benign lung tissues of patients with lung cancer].

Objective: To analyze the contents and distribution of 33 elements in the samples of lung cancer tissues, paracancerous lung tissues and benign lung tissues of patients with lung cancer.

Methods: 14 samples of lung cancer tissue, paracancerous lung tissue and benign lung tissue were collected from the patients with lung cancer in the high lung cancer rate area of our country. The samples were determined by inductively coupled plasma mass spectrometry after microwave digestion.

[Changes of proteomics in the lung of rats subjected to acrolein inhalation after enalapril administration].

Objective: To study the molecular mechanism of enalapril on rat chronic bronchopneumonia model with proteomics method.

Methods: Enalapril treatment applied to the recovery of chronic bronchopneumonia in rats, after enalapril administration, two-dimensional electrophoresis and mass spectrometry were performed to analyze the difference of the peptide finger print map and amino acid sequence of rat lung tissues in acrolein inhalation group and enalapril treatment group.

Results: Three differential protein spots from average 545 protein spots were found in lung tissues of two groups.

[Determination of thirty three elements in lung cancer tissues of patients with lung cancer by microwave digestion-ICP-MS].

A method for determining 33 elements in lung tissues of patients with lung cancer was developed by using vacuum freeze-drying microwave digestion-ICP-MS. The lung tissue samples were treated by vacuum freeze-drying equipment. After microwave digestion in HNO3-H2O2 solution system, the samples were diluted with the method of constant volume.

Treatment of metastatic spinal tumors by percutaneous vertebroplasty versus percutaneous vertebroplasty combined with interstitial implantation of 125I seeds.

Background: As the most frequent bone metastasis, spinal metastases cause severe pain and damage to vertebral bodies such as spinal osteolytic destruction and compression fractures. To avoid the trauma and complications of open surgery, a minimally invasive procedure, percutaneous vertebroplasty (PVP), has recently been developed to treat metastatic spinal tumors.

Purpose: To analyze the treatment outcomes of metastatic spinal tumors by percutaneous vertebroplasty (PVP) alone or PVP combined with interstitial implantation of 125I seeds.

[Effect of SspA on the formation of bacterial biofilm covering the surfaces of cardiovascular biomaterial Dacron].

This study sought to assess the effect of SspA on the formation of Staphylococcus aureus biofilm extending over the surfaces of Cardiovascular Biomaterial Dacron. SspA was extracted from the surface of staphylococcus aureus biofilm, purified, and then used to influence the adhesion of Staphylococcus aureus and the formation of Staphylococcus aureus biofilm on Dacron biomaterial surfaces. The formation of the Staphylococcus aureus biofilm on cardiovascular biomaterial Dacron surfaces under gradient SspA concentrations was evaluated by confocal laser microscopy.

[The role and significance of serum SYK and VEGF-C in metastasis of the lymph nodes of pulmonary adenocarcinoma.].

Background: Lung cancer has become one of the most dangerous malignant tumor in the world nowadays, whose pathogenesis is complex involving multi-genes and multi-elements. In this study, we try to explore the values of serum SYK and vascular endothelial growth factor-C (VEGF-C) in lymph node metastasis of lung adenocarcinoma.

Methods: Serum SYK and VEGF were examined in 160 lung adenocarcinoma patients with various metastasis lesions and 40 healthy volunteers by ELISA.

Nanoquartz in Late Permian C1 coal and the high incidence of female lung cancer in the Pearl River Origin area: a retrospective cohort study.

Background: The Pearl River Origin area, Qujing District of Yunnan Province, has one of the highest female lung cancer mortality rates in China. Smoking was excluded as a cause of the lung cancer excess because almost all women were non-smokers. Crystalline silica embedded in the soot emissions from coal combustion was found to be associated with the lung cancer risk in a geographical correlation study.

[Effects of selenium and reduced glutathione on the proliferation and apoptosis of XWLC-05 cell.].

Background: Selenium is an essential micronutrient for mammals but toxic in large amounts. Most studies indicate that selenium has inhibitory effect on cancer. The aim of this study is to investigate the effects of selenium and reduced glutathione (GSH) combined application on the proliferation and apoptosis of human lung adenocarcinoma cell line XWLC-05.

[Levels of activation markers of platelets in peripheral blood and their significance in patients with lung cancer].

Background: Platelet activation often occurs in intermediate and advanced tumors, with increases of expression and release of platelet adhesion molecule. The aim of this study is to investigate the expression of activation markers of platelet and their significance in lung cancer.

Methods: The activation markers of platelet, CD62P and CD63, were detected in peripheral blood of 120 patients with lung cancer and 60 healthy persons by FCM method.

A comparison of folic acid deficiency-induced genomic instability in lymphocytes of breast cancer patients and normal non-cancer controls from a Chinese population in Yunnan.

We hypothesized that the genomic response to folate deficiency might be different between breast cancer cases and healthy subjects. To test this hypothesis, we performed a comprehensive study on the genotoxic and cytotoxic effects of in vitro folic acid (FA) deficiency on primary human lymphocytes from 19 breast cancer patients and 20 age-matched healthy females from Yunnan, China using the cytokinesis-block micronucleus assay. Lymphocytes from the volunteers were cultured in RPMI1640 medium containing 30, 120 or 240 nM FA for 9 days.

[Expressions of epithelial growth factor receptor and E-cadherin in esophageal carcinoma and their correlation].

Background & Objective: Up-regulation of epithelial growth factor receptor (EGFR) and down-regulation of E-cadherin are correlated with genesis, progression, invasion, and metastasis of tumor; however,their expressions,especially their association,in esophageal carcinoma have seldom been reported. This study was to detect expressions of EGFR and E-cadherin in esophageal carcinoma, and to analyze their relationship.

Methods: Expressions of EGFR and E-cadherin in 50 specimens of esophageal squamous carcinoma and 8 specimens of esophageal adenocarcinoma were detected by immunohistochemistry.