Functions of Insulin-like Peptide Genes ( and ) in Female Reproduction of the Predatory Ladybird (Coleoptera: Coccinellidae).

Insulin-like peptides (ILPs) are important peptide hormones in insects, particularly involved in regulating physiological processes such as growth, development, and reproduction. However, the specific roles of ILPs in the reproduction of natural enemy insects remain unknown. In this study, two ILP genes, and , were cloned and their functions were analyzed in female L.

Potential Involvement of (Enterobacteriales, Enterobacteriaceae) in Biotype Differentiation of (Hemiptera: Aphididae).

, an obligate endosymbiont of most aphid species, can influence aphids' host adaptability through amino acid metabolism, potentially mediating biotype differentiation. However, its role in the biotype differentiation of remains unclear. To address this issue, six biotypes were tested in this study.

and homolog 1 Comediate the Action of Juvenile Hormone/ Signaling Pathway in Vitellogenesis of (J.E. Smith).

is a major migratory invasive pest and is of global concern. Vitellogenesis, a crucial process for population multiplication in oviparous insects, is regulated by endocrine hormones. In this study, three primary responders to JH signaling, the JH receptor gene , and the downstream transcription factor and , were first cloned and identified.

First Report of Botryosphaeria dothidea Causing Stem Blight of Dioscorea oppositifolia in China.

Dioscorea oppositifolia Thunb. (Chinese yam) is one of the traditional foods and medicinal plants in China. It has nutritional and medicinal value and plays an important role in treatment of diabetes and hypertension.

Molecular Characterization of Vitellogenin and Its Receptor in (J. E. Smith, 1797), and Their Function in Reproduction of Female.

The fall armyworm is a highly polyphagous invasive pest. The strong reproductive capacity is an important factor in the rapid colonization and expansion of Vitellogenin (Vg) and vitellogenin receptor (VgR) play important roles in insect reproduction. As the precursor of vitellin (Vn), Vg provides essential nutrition for embryonic development, and VgR mediates the uptake of Vg by oocytes.

Molecular Identification and Functional Characterization of Methoprene-Tolerant (Met) and Krüppel-Homolog 1 (Kr-h1) in Harmonia axyridis (Coleoptera: Coccinellidae).

Juvenile hormone (JH) plays a key role in regulating insect reproductive processes. Methoprene-tolerant (Met), as a putative JH receptor, transduces JH signals by activating the transcription factor krüppel homolog 1 (Kr-h1). To understand the effects of Met and Kr-h1 genes on female reproduction of natural enemy insects, the Met and Kr-h1 were identified and analyzed from Harmonia axyridis Pallas (HmMet and HmKr-h1).

Molecular Identification and Functional Characterization of Vitellogenin Receptor From Harmonia axyridis (Coleoptera: Coccinellidae).

Vitellogenin receptors (VgRs) have vital roles in reproduction by mediating endocytosis of the vitellogenin (Vg) in oviparous insects. Same as most insect species that possess only one VgR, in this study, a single VgR mRNA (HmVgR) was identified in an important natural enemy ladybeetle, Harmonia axyridis (Pallas). The open reading frame of HmVgR was 5,340 bases encoding a protein of 1,779 amino acids.

Selection and Evaluation of Reference Genes for qRT-PCR in (Lepidoptera: Noctuidae).

As an accurate and convenient technique, the qRT-PCR is always used in the quantitative expression analysis of functional genes. Normalization of the data relies on stable reference genes. The fall armyworm (J.

First Report of Alternaria tenuissima Causing Brown Spot Disease of Angelica dahurica in China.

Angelica dahurica (Fisch. ex Hoffm.) is an abundantly cultivated Chinese herbal medicine plant in China with about 4000 hectares grown, the annual production is up to 24,000 tons.