20 strains of bacteria were isolated from river sediment using enrichment culture medium for denitrification, and the denitrification intensity was determined. F10, one of bacteria strain, was identified having the highest denitrifying intensity, and further used to test its role in the removing of nitrogen and phosphorus from wastewater in laboratory. By checking the individual morphology, colony culture characteristics, DNA sequencing and 16S rDNA gene bank, F10 was identified as Alcaligenes faecalis, and its denitrifying intensity was 63.
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