[Studies on plantlet regeneration from the mature leaves of Dioscorea zingiberensis].

Objective: To establish a protocol of rapid clonal propagation of Dioscorea zingiberensis using mature leaves as explants.

Method: Out the optimal hormone combinations for callus induction, adventitious bud initiation, adventitious bud multiplication and rooting were found out by using MS medium with the macroelements at half strength as basal medium and studying the effects of BA, NAA and IBA on the processes.

Result And Conclusion: More than 80% of leaf explants formed callus when they were cultured on the media containing 1.