Publications by authors named "Yun-ju Gu"
Objective: To explore the relationship between the expression of EIIIA+ fibronectin in incised wound of rat's skin and injury time.
Methods: The wounding model was established by cutting the dorsal skin of 48 adult SD rats. The rats were sacrificed at the pre-set injury time as immediately, 0.
Objective: [corrected] To study the expression of EDA-FN variant in injured human skin fibroblast and its relation to wound healing.
Methods: Human skin fibroblast cells were separated from human skin and cultured and monoclonal antibody to fibronectin [IST-9] was applied to amplify target segments.
Results: Trace amount of EDA-FN was detected in the culture cells.
[The research of relationship between DNA degradation and postmortem interval].
Fa Yi Xue Za Zhi
May 2005
Objective: To investigate the relationship between the DNA degradation in cells and postmortem interval.
Methods: Tissue pieces of the heart, liver, spleen and kidney of one corpse (male, 54 years old, died of mechanical injury, PMI of 6h) were obtained in 6, 12, 24, 48 h after death, fixed in Carnoy fluid, and then paraffin sections were prepared, stained with Feulgen and analyzed by Image analysis technology (IAT). Meanwhile the single-cell suspension of tissues of the man was prepared and inspected by FCM after PI stained.
Objective: To investigate the signification of CTnI in acute myocardial infarction.
Methods: The model of myocardial infarction was established by ligating the left ventricle branch. Immunohistochemistry and image analysis were used to detect the change of CTnI in the model, and The sensitivity of immunohistochemistry and HE coloration were also compared.
Objective: To estimate the effort between antisense RNA probe and random primed DNA probe.
Methods: The in situ hybridization was conducted on parafin section from wounding model of rat skin.
Results: Although both probes appeared positive staining, RNA probes was superior to DNA probes in terms of depth of staining and background.
[The expression of FN-IIIcs in the wound healing and its relationship with injury time].
Fa Yi Xue Za Zhi
February 2002
Objective: To research the expression of alternative splicing segment of fibronectin-IIIcs in incised wound of skin, to offer the experimental data for early injury time judgement.
Methods: Using in situ hybridization with DIG-labeled anti-sense RNA probe, the expression of FN-IIIcs domain was detected in both human and rat skin incised wound.
Results: (1) The expression of FN-IIIcs improved from the time of 30 min after injury, reached peak at 6 h and then reduced gradually.