[Photolysis of Gaseous HNO3 on the α-Fe2O3 Films Under 308 nm UV Light].

Under 308 nm UV light, photolysis of HNO3 in the gas phase and on the α-Fe2O3 films was studied by using ultraviolet-visible spectrophotometer (UV-Vis), Fourier transform infrared spectrometer (FTIR) combined with ion chromatography (IC) technique. The effects of HNO3 initial concentration, relative humidity (RH) and illumination time were systematically investigated. Results showed photolysis of HNO3 could produce NO2 and NO in the gas phase and on the α-Fe2O3 films.

[Quantitative detection of human cytomegalovirus in aggressive and chronic periodontitis lesions].

Objective: To establish a quantitative real-time PCR assay for the detection of human cytomegalovirus (HCMV) DNA load in subgingival specimens from the patients with aggressive and chronic periodontitis, and to investigate the relationship between HCMV infection and the periodontal status.

Methods: A total of 114 subgingival plaque specimens were taken from 18 subjects with aggressive priodontiti (AgP), 24 subjects with chronic periodontitis (CP) and 15 healthy control subjects. Standard quantification was performed with recombinant plasmid containing a conserved fragment of HCMV.

Replication and transcription of human papillomavirus type 58 genome in Saccharomyces cerevisiae.

Background: To establish a convenient system for the study of human papillomavirus (HPV), we inserted a Saccharomyces cerevisiae selectable marker, Ura, into HPV58 genome and transformed it into yeast.

Results: HPV58 genome could replicate extrachromosomally in yeast, with transcription of its early and late genes. However, with mutation of the viral E2 gene, HPV58 genome lost its mitotic stability, and the transcription levels of E6 and E7 genes were upregulated.

Two cysteine proteinases respond to bacterial and WSSV challenge in Chinese white shrimp Fenneropenaeus chinensis.

The cDNAs encoding CathL and legumain from Chinese white shrimp Fenneropenaeus chinensis (FcCathL, FcLegu) were obtained. Both FcCathL and FcLegu mRNA were expressed mainly in the hepatopancreas of unchallenged shrimp. Time-course analysis of FcCathL showed that FcCathL was upregulated in the hepatopancreas of shrimp challenged with white spot syndrome virus (WSSV) at 12 h.

A hepatopancreas-specific C-type lectin from the Chinese shrimp Fenneropenaeus chinensis exhibits antimicrobial activity.

Lectins play important roles in animal innate immune responses by serving as pattern recognition receptors, opsonins, or effector molecules. Here, we report a novel hepatopancreas-specific C-type lectin, designated Fc-hsL, from the hepatopancreas of the Chinese shrimp, Fenneropenaeus chinensis. The cDNA of Fc-hsL is 571 bp long with a 480 bp open reading frame that encodes a 159-residue protein.

Molecular cloning and characterization of Fc-TSP from the Chinese shrimp Fennerpenaeus chinensis.

Thrombospondins (TSPs) are extracellular, multidomain, calcium-binding glycoproteins that modulate cell behavior in homeostasis and during development, wound-healing, immune response and tumor growth of adult tissues in vertebrates. In invertebrates these proteins are a major component of cortical rods in mature oocytes. A fragment of a thrombospondin-like gene was generated by screening a subtractive cDNA library constructed from the hemocytes of Chinese shrimp, Fennerpenaeus chinensis.