Targeting USP47 overcomes tyrosine kinase inhibitor resistance and eradicates leukemia stem/progenitor cells in chronic myelogenous leukemia.

Identifying novel drug targets to overcome resistance to tyrosine kinase inhibitors (TKIs) and eradicating leukemia stem/progenitor cells are required for the treatment of chronic myelogenous leukemia (CML). Here, we show that ubiquitin-specific peptidase 47 (USP47) is a potential target to overcome TKI resistance. Functional analysis shows that USP47 knockdown represses proliferation of CML cells sensitive or resistant to imatinib in vitro and in vivo.

Wu-5, a novel USP10 inhibitor, enhances crenolanib-induced FLT3-ITD-positive AML cell death via inhibiting FLT3 and AMPK pathways.

The kinase FLT3 internal tandem duplication (FLT3-ITD) is related to poor clinical outcomes of acute myeloid leukemia (AML). FLT3 inhibitors have provided novel strategies for the treatment of FLT3-ITD-positive AML. But they are limited by rapid development of acquired resistance and refractory in monotherapy.

Characterization of naturally occurring pentacyclic triterpenes as novel inhibitors of deubiquitinating protease USP7 with anticancer activity in vitro.

Deubiquitinating protease USP7 is a promising therapeutic target for cancer treatment, and interest in developing USP7 inhibitors has greatly increased. In the present study, we reported a series of natural pentacyclic triterpenes with USP7 inhibitory activity in vitro. Among them, both the ursane triterpenes and oleanane triterpenes were more active than the lupine triterpenes, whereas ursolic acid was the most potent with IC of 7.

[Study on the prediction of soil heavy metal elements content based on mid-infrared diffuse reflectance spectra].

The present paper analyzed the possibility of mid-infrared diffuse reflectance spectra for quick assessment of heavy metal element content in soil quickly. Soil samples were collected from Jiangning District and Baguazhou Island, and the numbers of sample were 103 and 58 separately. Jiangning District samples were used as calibration set while Baguazhou Island samples as validation set.

[Research on spectrum denoising methods based on the combination of wavelet package transformation and mathematical morphology].

The present study introduced the generalized morphological filter into the denoising of visible and near infrared spectra for the first time, and provided a new method for denoising the reflectance spectra by combining mathematical morphology methods with the wavelet packet transformation. The authors used vegetable spectra from USGS spectral library as the reference spectra, and obtained the noised spectra by adding noises with different signal-to-noise ratios to the referenced spectra. The results were evaluated by signal-to-noise ratio (SNR), root mean squared error (RMSE), normalized correlation coefficient (NCC) and smoothness ratio (SR) of the denoised spectra.

[A study of environmental changes on new snail habitat though multi-temporal relative radiometric normalized TM images].

Objective: To study the environmental changes of new snail habitual by using multi-temporal relative radiometric normalized TM images taken in Wuzhoutou of Nanchang, Jiangxi province.

Methods: TM images of Poyang Lake were collected on 3rd April 1998, 16th April 2000 and 5th May 2004. Taking images in 2004 as a reference, the TM images in 1998 and 2000 were radiometric normalized.

Feasibility of reflectance spectroscopy for the assessment of soil mercury contamination.

Conventional methods for investigating soil Hg contamination based on raster sampling and chemical analysis are time-consuming and relatively expensive. The objective of this study was to develop a rapid method for investigating Hg concentration in suburban agricultural soils of the Nanjing region using reflectance spectra within the visible-near-infrared (VNIR) region. Several spectral pretreatments (absorbance, Kubelka-Munk transformations and their derivatives) were applied to the reflectance spectra to optimize the accuracy of prediction.