[Effect of different isoforms of tocopherols on expression of intercellular adhesion molecule-1 in human umbilical vein endothelial cells].

Objective: To observe the influence of different tocopherol isoforms on oxidized low density lipoprotein (oxLDL) or recombinant human C-reactive protein (rhCRP)-induced expression of intercellular adhesion molecule-1 (ICAM-1) in human umbilical vein endothelial cells (HUVECs) and to investigate the potential mechanisms and effects of different tocopherols on atherosclerosis.

Methods: Cultured HUVECs were incubated with oxLDL, oxLDL + alpha-tocopherol, oxLDL + gamma-tocopherol, oxLDL + mixed-tocopherols, rhCRP, rhCRP + alpha-tocopherol, rhCRP + gamma-tocopherol rhCRP + mixed-tocopherols for 24 hours, respectively. The ICAM-1 expressions of protein and mRNA were detected by cell enzyme linked immunosorbent assay (ELISA), flow cytometric technique and RT-PCR.